Chemical constituents from Rehmannia glutinosa / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents from the roots of Rehmannia glutinosa.</p><p><b>METHOD</b>The compounds were isolated by various chromatographic methods and identified by spectroscopic analysis.</p><p><b>RESULT</b>Twelve compounds were isolated and their structures were identified as 5-hydroxymethyl-pyrrole-2-carbaldehyde (1), 5-hydroxymethyl furfural (2), tyrosol (3), 5,6-dihydroxy-beta-ionone (4), 6-O-E-feruloyl ajugol (5), acteoside (6), leucosceptoside A (7), martynoside (8), isomartynoside (9), purpureaside C (10), jionoside A1 (11), and jionoside B1 (12).</p><p><b>CONCLUSION</b>Compounds 1, 3 and 9 were isolated from the genus Rehmannia for the first time.</p>

Determination of carnosic acid in rat stomach and intestine by high performance liquid chromatography method / 中国中药杂志

<p><b>OBJECTIVE</b>To establish a HPLC method to determine the carnosic acid in the stomach and intestine of rats and study its tissue distribution characteristics.</p><p><b>METHOD</b>After intragastric administration of carnosic acid (90 mg x kg(-1)), rats for each time-point were sacrificed by decapitation. After removal of the blood, various tissues were rapidly removed and weighted, all tissues were treated with a series of pretreatment before HPLC. Chromatographic separation was achieved on a Kromasil C18 column (4.6 mm x 150 mm, 5 microm) protected by an ODS guard column at 25 degrees C, using acetonitrile-0.1% phosphoric acid solution (55:45) as mobile phase, at a flow rate of 1 mL x min(-1). The wavelength of the UV detector was set at 210 nm for carnosic acid and internal standard.</p><p><b>RESULT</b>Good linearities were obtained in every tissue over a range of 0.3212-160.6 mg x L(-1). The recovery, intra-day and inter-day precision and accuracy of three concentrations of carnosic acid in tissues met the requirements of methodology. And the stability of the tissue samples were also validated. The results of distribution in stomach and intestine showed that the highest concentration was (307.1 +/- 119.2) microg x g(-1) in stomach and (33.32 +/- 17.70) microg x g(-1) in intestine after intragastric administration of carnosic acid.</p><p><b>CONCLUSION</b>The HPLC method was established to determine the concentration of carnosic acid in tissues. This method is quick, precise, and reproducible. It is the first time to study the tissue distribution of carnosic acid in rats after intragastric administration.</p>

Chemical constituents of flavonoids from rhizome of Sophora tonkinensis / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents of the flavonoids from Sophora tonkinensis.</p><p><b>METHOD</b>The compounds were isolated by chromatography on silica gel, Sephadex LH-20 column and identified by spectroscopic analysis.</p><p><b>RESULT</b>Eight compounds were isolated and their structures were identified as tonkinochromane I (1), glabrol (3), lupinifolin (2), tonkinensisol (4), 8-C-prenylkaempferol (5), 7,2'-dihydroxy-4'-methoxy-isoflavanol (6), formononetin (7), and genistein (8), respectively.</p><p><b>CONCLUSION</b>Compound 1 was a new compound. And compound 6 was firstly isolated from the genus Sophora. Compounds 2, 3 and 5 were isolated from S. tonkinensis for the first time.</p>