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Objective:To construct a rat model of hypothalamic obesity by two point electrical damage to the ventromedial hypothalamus and arcuate nucleus.Methods:Twenty adult male SD rats of SPF grade were randomly divided into experimental group and sham operation group.A 25GA (0.45 mm) solid iron needle was used, the needle was coated with an insulating layer, and the tip exposed a 0.5 mm conductive area.With reference to The Rat Brain in Stereotaxic Coordinates and using the stereotactic instrument (AP: -2.6 mm, ML: ± 0.6 mm, DV: -9.6 mm) as the coordinate, 1.5 mA current was continuously applied for 25 s, the ventromedial nucleus (VMH) and arcuate nucleus (ARC) of bilateral brain in SD rats was damaged.During the experiment, the body weight(BW), food intake(FI) and water intake(WI) of the two groups were recorded regularly.The rats were sacrificed on the 28th day after the operation, and the changes of periprenal fat mass and body length were measured.The changes of liver and adipose tissue were detected by HE staining method, leptin by ELISA, leptin receptor(LEPR) by Western blot.Results:(1) The body weight of rats in the experimental group ((427.5±17.7)g) and weight gain ((208.5±14.8)g) were significantly increased compared with the rats in the control group((349.2±17.7)g), ((136.2±21.4)g)on the 28th day after operation ( t=7.661, 6.806, both P<0.001). (2) The daily food intake of rats in the experimental group ((44.2±6.6)g) on the 28th day after surgery was significantly higher than that in the control group ((23.0±3.6)g) ( t=6.918, P<0.001). There was no significant difference of the daily drinking water of rats between experimental group((37.5±12.1)ml) and the control group ((35.0±11.8)ml) ( t=0.361, P=0.726). (3) Perikidney fat mass of experimental group rats ((13.4±2.7)g) significantly increased 28 days after operation compared with control group rats((6.3±0.9)g)( t=4.250, P<0.05). The naso-anal length of experimental group((21.8±0.4)cm) was significantly decreased compared with the control group ((23.4±0.2)cm) ( t=-6.788, P<0.01). The Lee index of the experimental group (348.9±8.5) was significantly higher than that of the control group(305.5±4.3)( t=7.898, P<0.01). (4) The serum leptin content ((8 324.10±159.00)μg/L) of the experimental group rats at 28 days after surgery was significantly higher than that of the control group((2 705.31±407.10)μg/L) ( t=25.712, P<0.001). The lateral hypothalamus area (LHA) LEPR protein expression (1.3±0.1) in the experimental group was significantly higher than that in the control group (0.9±0.1) ( t=4.932, P<0.01). Conclusion:Two-point electrical damage to bilateral VMH and ARC of rats can establish hypothalamic obese rat model.
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Objective To explore the use of the transabdominal ultrasound with different frequency scanning anterior lower segment uterine scar situation for maternal guidance .Methods Pregnant women had a history of cesarean section 323 cases, and were lower uterine segment transverse incision .Of which seven cases of cesarean section were 8 months to 12 months, 49 patients 13 months to 24 months, 89 patients 25 months to 36 months, and more than 169 cases of 37 months.High-frequency ultrasound was use for ab-dominal and dynamic monitoring of different-year anterior lower segment uterine scar thickness and healing .Pregnancy outcomes ob-served were compared with surgery .Results With the extension of cesarean time , grade I uterine scar was also increased to 25~36 months (2 years ) which was the most obvious , and grade II and III scars were reduced accordingly .When group 25~36 months was used as the control group , there was statistically significant difference relative to group 8 ~12 months and 13 ~24 months ( P 37 months ( P >0.05).Among 323 cases, seven cases had uterine rup-ture that was confirmed by surgery with a rupture rate of about 2.1%.The rupture rate was about 28.6%in group 8~12 months, a-bout 2%in group 13~24 months, about 1.0%in group 25~36 months, and about 1.2%in group >37 months.When group 25~36 months was used as the control group , there was statistically significant difference relative to groups 8~12 months and 13~24 months ( P 37 months ( P >0.05 ) .Conclusions Abdominal ultra-sound with different frequency scanning can monitor the thickness and continuity of anterior lower segment uterine scar to estimate the scar healing situation , and guide uterine scar grading .Therefore, it is great significance in guiding clinical decision of motherhood ap-proach.
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The length of cervical canal was monitored by perineal ultrasonography in 377 pregnant women with 22 to 36 weeks of gestation,at the same time the contents of fibronectin (fFN) in cervical secretions were measured.The rate of cervical canal length shortening from the time of first visit to the appearance of preterm signs was calculated.When the cervical length shortening rate was 16％ ～ 60％,fFN (+) was correlated with higher preterm birth rate (P ＜0.01),while when the shorten rate ＞ 60％ there was no significant difference in preterm delivery rate between fFN (+) and fFN (-) groups (P ＞ 0.05).The results indicate that the monitoring of cervical canal length combined with fiberonectin measurement in cervical secretion may better predict pretenn birth.
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<p><b>OBJECTIVE</b>To study the prevalence and the epidemiological feature of hepatitis B in the population of Zaozhuang and the seroepidemiology of hepatitis B virus infection.</p><p><b>METHODS</b>HBsAg, anti-HBs and anti-HBc were detected in 963 sera collected from 312 families by RIA, which were selected by multistage and stratified cluster sampling.</p><p><b>RESULTS</b>The results indicated that the standardized positive rates of HBsAg, anti-HBs and anti-HBc were 7.08%, 37.56% and 41.35%, respectively and the standardized prevalence rate of hepatitis B virus infection was 44.37%. The positive rate of HBsAg was higher in male subjects and in the urban area groups than those in female subjects (P<0.05) and in rural area groups (P<0.01), respectively. There was no significant difference in the positive rate of HBsAg among all age groups and occupation groups (P>0.05). The positive rates of anti-HBs and anti-HBc and infective rate of hepatitis B virus increased with age. The infective rate of hepatitis B virus was higher in male subjects and in rural area groups than those in female subjects (P<0.05) and in urban area groups (P<0.05), respectively.</p><p><b>CONCLUSIONS</b>The HBV infective rate of population was higher in Zaozhuang city; measures of prevention and control should be taken actively and effectively.</p>