Determination of zolpidem tartrate tablets after radiation by UHPLC / 药学实践杂志

Objective To establish a UHPLC method for the determination of zolpidem tartrate tablets after radiation, and to investigate the effect of different radiation doses on the content of zolpidem tartrate tablets. Methods Ultra high performance liquid chromatography was used. The content of zolpidem tartrate tablets irradiated by γ-ray was determined. Using C18 column, acetonitrile methanol-0.05 mol/L phosphoric acid solution (the pH value as 5.5 with triethylamine) (18∶26∶56) was used as the mobile phase. The flow rate was 0.7 ml/min, and the detection wavelength was 254 nm. Results The method validation showed good linearity in the concentration range of 5-80 μg/ml (r=0.999 6); The average recovery was 98.2%, RSD was 1.72%, and the repeatability was 0.87%. The contents of zolpidem tartrate were 105.1%, 106.4%, 102.7% and 105.4% under 0, 8, 25 and 80 kGy radiation. Conclusion UHPLC has accurate results with short analysis cycle in this study. It is suitable for the determination of zolpidem tartrate tablets after radiation. The content of zolpidem tartrate tablets remained basically unchanged after radiation.

Comparative Study on the Effect of Raw Product and Different Processed Products of Gekko gecko on Kidney- yang Deficiency Model Mice Induced by Adenine / 中国药房

OBJECTIVE：To compare the effects of raw pro duct and different processed products of Gekko gecko on kidney-yang deficiency model mice induced by adenine. METHODS ：Totally 100 mice were randomly divided into blank group （n＝10）and modeling group （n＝90）. Modeling group was given adenine （50 mg/kg）intragastrically for 10 days to induce kidney-yang deficiency model ；blank group was given normal saline （0.2 mL/10 g）intragastrically. After modeling ，70 mice were randomly divided into model group ，positive group （Jinkui shenqi pill ，6.4 g/kg），G. gecko crude product group （1.2 g/kg）， wine-processed G. gecko group（1.2 g/kg）and oil-processed G. gecko group（1.2 g/kg）according to body weight and symptoms of kidney-yang deficiency ，with 14 mice in each group. Blank group and model group were given constant volume of normal saline intragastrically；administration groups were given relevant medicine intragastrically （0.2 mL/10 g），once a day ，for consecutive 14 d. During the experiment ，the symptoms and signs of mice in each group were observed. After last medication ，renal index ，testis index and serum levels of T ，CORT，BUN and Cr were measured ；HE staining method was used to observe the pathological changes of renal tissue of mice in each group. RESULTS ：Compared with blank group ，the mice in the model group suffered from performance of kidney-yang deficiency ，such as weight loss ，crouch and arch back ，chills and cold limbs ，and sparse body hair ， while renal index and serum levels of BUN and Cr were increased significantly （P＜0.01）. In renal tissue ，there were BA28117 pathological damages such as irregular arrangement of renal KY2016YB211tubular epithelial cells ，light staining of nucleus and edema of cytoplasm. Compared with model group ， performance of kidney-yang deficiency was improved to different extents in G. gecko crude product group and processed product groups（especially in wine-processed G. gecko group）；serum levelsof BUN and Cr were decreased significantly （P＜0.05 or P＜0.01）；pathological damage of renal tissue was alleviated in different degrees. In addition ，body weight of mice was increased significantly in G. gecko processed products groups （P＜0.01），and renal indexes of mice were decreased significantly in G. gecko crude product group and processed products groups （P＜0.05 or P＜0.01）. Compared with G. gecko crude product group ，renal index ，serum levels of BUN and Cr were significantly decreased in wine-processed G. gecko group（P＜0.01），and serum level of Cr was significantly decreased in oil-processed G. gecko group（P＜ 0.05）. CONCLUSIONS ：G. gecko crude product ，wine-processed G. gecko and oil-processed G. gecko all show a certain improvement effect on kidney-yang deficiency mice induced by adenine ，especially wine-processed G. gecko .

The efficacy of calcined natural bovine bone(CBB)in the treatment of bone defects in animal / 实用口腔医学杂志

Objective:To observe the efficacy of calcined bovine bone(CBB)in the repair of animal bone defects.Methods:Ca-nine alveolar bone defect model,rat and rabbit calvarial critical size defect models were established respectively.All animals were randomly divided into 2 groups(n=6).The defects in the experimental group were repaire with CBB,those in the control group were not treated.The effects were observed by HE staining,Micro CT and Masson trichrome staining.Results:Canin alveolar bone de-fects were well repaired 8 week after operation.The skull defects in rabbits and SD rats were replaced by new bone 1 2 and 8 weeks after operation respectively.In all the control groups bone defects were not healed or not completely repaired.Conclusion:CBB is ef-fective in the repair of bone defects.

Treatment of Tumor in Nasopharynx and the Base of Nasal Cavity by Hard Palate Approach Through Endoscopic Surgery / 医学研究杂志

Objective To evaluate the practical value tumor from nasopharynxl and the base of nasal cavity . Methods 12 patients , among these 5 cases suffered from the tumor of the base of nasal cavity and 7 from nasopharynx , were treated from January 2001 to January 2005 . After general anesthesia the tumors were incised by hard palate approach , and the preoperative selective intraarterial embolization were used in three nasopharyngeal tumors, and The controlled hypotension were used during surgery ifnecessary.Results Two malignant tumors were changed the type of surgery after the operation quick freeze pathologic examination. The mucosal flaps of other 10 cases survived and the function of phonation、swallowing、respiration and mastication were normal. The patients remained free of disease after follow-up period of over 1 year. Conclusions The endoscopic managent of resection tumor of nasopharynxl and the base of nasal cavity by hard palate approach have great advantage to traditional hard palate approach.

Disinfection effect of root canal with non-filling medication in root canal therap / 中国基层医药

Objective To observe the clinical disinfection effect of root canal with non-filling medication.Methods 50 patients who required root canal therapy for apical period(acute or chronic) or pulp necrosis were involved in the study.Non-filling medication on the root canals were taken bacteria from the root canals were collected and cultured before and after the treatment.Results Bacteria were detected in every specimen,which were mixture of aerobes and anaerobes.One week later,only two strain were detected in two specimen.Conclusion Non-filling medication can be used as an effective disinfection to sterilize root canal.