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Objective To study the differences in intestinal flora of normal and type 2 diabetic mice, the effect of alcoholic extract of Coreopsis tinctoria Nutt on mouse intestinal flora, and explore the possible relationship between alcoholic extract of Coreopsis tinctoria Nutt, intestinal flora and type 2 diabetes mellitus (T2DM) in mice.Methods Stool samples were collected from the normal control group (A), high dose (1.8 g/kg) (B) and moderate dose (1.2 g/kg) (C) alco-holic extract of Coreopsis tinctoria Nutt model groups, metformin (0.2 g/kg) treatment group (D) and blank control (E) group.16S rDNA real-time quantitative PCR assay was used to determine the levels of Clostridium coccoides and Bacteroides thetaiotaomicron in the stool samples.Pearson analysis method was used to analyze the correlation between the levels of tar-get bacterial species and the fasting blood glucose ( FBG) in the mice.Results 1.Compared with the normal control group, the levels of Clostridium coccoides and Bacteroides thetaiotaomicron in the T2DM model group were significantly low-ered (P=0.017, P=0.002).2.Compared with the model group, the levels of Clostridium coccoides and Bacteroides the-taiotaomicron of the high dose Coreopsis tinctoria Nutt alcohol extract group were significantly different ( 2 weeks: P =0.027, P=0.006;4 weeks:P=0.007, P=0.012).3.The levels of Clostridium coccoides and Bacteroides thetaiotaomi-cron were positively correlated with the FBG level in the mice.Conclusions The alcohol extract of Coreopsis tinctoria Nutt has certain effect on the intestinal flora in type 2 diabetic mice and there is certain correlation between the effect of alcohol extract of Coreopsis tinctoria Nutt and their blood glucose level.
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[ABSTRACT]AIM:Toinvestigatetheroleofcysteine-rich61(Cyr61/CNN1)inproliferationandmigrationof bone marrow mesenchymal stem cells ( BMSCs ) .METHODS: The lentiviral vector carrying CCN 1 ( Lenti-GFP-CCN1 ) was constructed and then transfected into the rat BMSCs .The cells were divided into non-transfection group , transfection group ( transfected with Lenti-GFP-CCN1 ) and negative control group ( Lenti-GFP ) .The fluorescence intensity of the transfected BMSCs was observed under inverted fluorescence microscope .The effects of CCN1 on the proliferation and mi-gration of BMSCs were detected by MTT assay and scratch wound healing assay .RESULTS:The proliferation of BMSCs transfected with Lenti-GFP CCN1 had no significant difference compared with negative control group and control group .The width/thickness ratio of migrated BMSCs in wound healing was significantly higher in Lenti-GFP-CCN1 group than that in negative control group and control group (P<0.05).CONCLUSION:Exogenous CCN1 promotes the migration of BMSCs.
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AIM To study the protective effects of trigonella foenum graecum(TFGs) on acute cerebral ischemia. METHODS Acute incomplete ischemia was induced by ligaturing bilateral carotid arterise and cutting heads in mice, the survival time and asthmatic time were observed; the coagulation timewas measured by sheet glass method,and the blood viscosity was also assayed; the platelet aggregation induced by collagen were studied by turbdimetry in vitro. RESULTS TFGs prolonged survival time, coagulation time and asthmatic time significantly and inhibited platelet aggregation ratio in rabbit,and decreased the blood viscosity. CONCLUSION TFGs has the protective effects on acute cerebral ischemia.
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S AIM To study the protective effects of trigonella foenum graecum(TFGs) on acute cerebral ischemia. METHODS Acute incomplete ischemia was induced by ligaturing bilateral carotid arterise and cutting heads in mice, the survival time and asthmatic time were observed; the coagulation time was measured by sheet glass method,and the blood viscosity was also assayed; the platelet aggregation induced by collagen were studied by turbdimetry in vitro . RESULTS TFGs prolonged survival time, coagulation time and asthmatic time significantly and inhibited platelet aggregation ratio in rabbit,and decreased the blood viscosity. CONCLUSION TFGs has the protective effects on acute cerebral ischemia.