RESUMO
Objective To prepare cysteamine group substitutes of catechin compounds from Weimaining. Methods After thiolased by cysteamine, Weimaining was subjected to the open column chromatography on C18, and subsequently to the semi-preparative high performance liquid chromatograph (HPLC) to prepare the cysteamine group substitutes of catechin compounds. Results Compounds 4β-(2-aminoethylthio)catechin, 4β-(2-aminoethylthio)epicatechin, and 4β-(2-aminoethylthio) epicatechin-3-O-gallate were obtained, and their purities were above 95%. Conclusion The method is simple and easy, and provides a reference for the preparation of the cysteamine group substitutes of catechin compounds.
RESUMO
AIM: To establish the determination method of chlorogenic acid and baicalin in Ganmaozhike Granules (Flos Lonicerae,Radix Scutellariae,etc.). METHODS : The contents of chlorogenic acid and baicalin in Ganmaozhike Granules were determined by HPLC. RESULTS : The linear velation was good. The average recovery of chlorogenic acid was 101.9%,RSD was 1.12%;The average recovery of baicalin was 99.2%,RSD was 1.31%. CONCLUSION : The method is simple,accurate and with a good reproducibility and can be used for the quality control of the granules.