Reform and practice of precise teaching for experiment in medical laboratory technology / 中华医学教育探索杂志

In order to train professionals in medical laboratory technology who are directly engaged in medical examination and medical laboratory work and who have strong practical ability and can adapt to the development of precision medicine, the reform of precise teaching for experiment is implemented. Through the precise stratification of experimental projects and knowledge points, the design of precise teaching activity and the construction of precise evaluation system for experiments, guided by precision medicine during the experimental process, the standardization and precision training of basic skills for students are strengthened in clinical laboratory test. The students can more well adapt to the needs of society for technical and innovative talent in the new period. The precise teaching for experiment will become one of the main characteristics of medical laboratory technology education in our school.

Detection of p16/Ki-67 in women with abnormal cytological results and its diagnostic value for cervical intraepithelial neoplasia II+ grade / 国际生物医学工程杂志

Objective:To explore the detection capability of p16/Ki-67 double staining technique in women with various abnormal thinprep cytologic test (TCT) results and its diagnostic value for cervical intraepithelial neoplasia Ⅱ+ grade (CIN2+).Methods:A total of 225 women with abnormal TCT results, i.e. the atypical squamous cells of undetermined significance(ASC-US), in the Tianjin Central Hospital of Gynecology Obstetrics, Nankai University Affiliated Maternity Hospital from December 2018 to December 2019 were enrolled. p16/Ki-67 double staining were detected and compared with the high risk human papillomavirus (HR-HPV) and pathological results.Results:The positive rates of p16/Ki-67 double staining increased with cytologic and pathologic categories. For diagnosis of CIN2+, p16/Ki-67double staining (90.1%) was less sensitive than HR-HPV testing (98.2%)( P<0.05), but the specificity of p16/Ki-67 double staining (58.8%) was significantly higher than HR-HPV(21.6%) ( P<0.001). Conclusions:Compared with HR-HPV, p16/Ki-67 double staining has better effect on diagnosing CIN2+. p16/Ki-67 double staining can be considered as triaging method for management of ASC-US and LSIL patients, significantly reduce the colposcopy referral rate (nearly 50%), which has high clinical application value.

Meta analysis of curative effect of safflower yellow injection on the elderly with early diabetic nephropathy / 中华老年医学杂志

Objective To systematically evaluate the effects of safflower yellow injection on the treatment of senile early diabetic nephropathy. Methods All databases from January 2000 to June 2017,including the Chinese biomedical literature database(CBM),Chinese Journal Full-text Database (CNKI),Chinese PSTP VIP(VIP)and Wanfang database(WFSD),were searched on the safflower yellow injection for treatment of senile early diabetic nephropathy in randomized controlled trail.Literatures were selected based on inclusion and exclusion standards defined in advance,and their quality was evaluated.The valid data were retrieved and a Meta analysis of the urinary albumin excretion rate and serum creatinine was undertaken using RevMan5.3 Software. Results 16 articles with 1 048 cases were internalized into the study.Among 1 048 cases,525 cases were treated with safflower yellow injection added to general comprehensive treatment(treatment group),and 523 cases with general comprehensive treatment(control group).The results of meta analysis showed that urinary protein excretion rate 〔WMD(weighted mean difference)= -0.74,95% CI:-0.85 to -0.63;Z=13.15,P=0.000〕 and serum creatinine level 〔W M D= -4.30,95% CI:-6.54 to -2.06,Z=3.77,P= 0.002〕 were significantly lower in safflower yellow injection added to conventional treatment than in conventional treatment. Conclusions Safflower yellow pigment injection is superior to conventional treatment for senile early diabetic nephropathy,but there are few high quality studies and the sample size is limited,therefore more studies are needed.

Clinical and imaging characteristics of pediatric neuromyelitis optica spectrum disorders / 中华实用儿科临床杂志

Objective To analyze the clinical and imaging characteristics of pediatric neuromyelitis optica spectrum disorders(NMOSD)in children. Methods The clinical data,imaging manifestations and follow - up data of 16 NMOSD patients at Department of Pediatric Neurology,Shandong Provincial Hospital Affiliated to Shandong Univer-sity between July 2013 and September 2017 were respectively analyzed. Results In 16 patients,initial presentations included optica neuritis(ON)in 5 cases,longitudinally extensive transverse myelitis(LETM)in 6 cases,and among them there were 2 cases with acute disseminated encephalomyelitis and 3 cases with both ON and LETM. Eleven cases received aquaporin - 4(AQP4)antibody examination and 4 cases were found seropositive. One case out of 7 detected cases was found AQP4 antibody positive in cerebrospinal fluid. Eleven cases received optica magnetic resonance imaging (MRI),and 8 cases were found abnormal signals in optic nerve and optica chiasma. The spinal cord MRI showed 13 ca-ses with LETM manifestations,and abnormal signals were found in vertebral segments(5 - 13),and among them 1 case had cervical cord,3 cases were thoracic cord and 9 cases were both of the above. Lesions in the cervical cord in 2 cases were extended upward to the medulla. Fifteen cases received brain MRI and all of them had brain lesions,which were mainly involved in the central and subcortical white matter,thalamus,corpus callosum,brainstem,the junction of spinal cord and medulla,cerebellum,and so on. All patients received treatment for acute attacks with high - dose Methylpred-nisolone and/ or gamma globulin and got obvious relief. Two cases with recurrent ON received treatment of Rituximab and their vision became improved. Fifteen patients were followed up,and 2 cases had limb disorders and 4 cases had visual impairment,other patients had no clinical symptoms. Conclusions Pediatric NMOSD has a diverse clinical pre-sentation at the onset disease. Those who are initial diagnosed acute myelitis,ON and acute disseminated encephalomye-litis should be considered the possibility of NMOSD. Antibody to AQP4 testing can assist the diagnosis. The typical ima-ging characters of NMOSD children are abnormal signals in the high expression area of AQP4. Intracranial lesions are more common in children. The acute treatment includes the high - dose Methylprednisolone and gamma globulin. Rituximab can be used for the recurrent patients.

Reform of the three-stage experimental teaching of medical laboratory technology / 中华医学教育探索杂志

In order to adapt to the training target of medical laboratory technology professionals under the condition of five to four educational system,we reformed the experimental teaching of medical laboratory technology.The experiment teaching was divided three phases (traditional classroom teaching + simulated clinical laboratory training + clinical laboratory teaching).Classroom teaching focused on the cultivation of students' basic skills test;simulation laboratory training focused on strengthening the students' operating skills and basic post ability and professional quality;hospital laboratory teaching focused on strengthening the students' ability of medical laboratory management,test comprehensive analysis and scientific research.The reform practice of two sessions of the students showed that the students' examination skills and comprehensive quality improved significantly,which could better adapt to the social demand for technical personnel.

Characteristics change of the human directional highly lymphatic metastasis ovarian carcinoma cell and venous endothelial cell after establishment of their condition cultrue and co-culture cell system / 中华妇产科杂志

Objective To establish the condition cultrue cell system and co-culture cell system with SKOV3/PM4,HUVEC and to study the changes of their biological characteristics. Methods The cells of SKOV3/PM4 and HUVEC were labeled with green and red fluorescent respectively. The cell supernatant of SKOV3/PM4 and HUVEC were collected respectively as the condition medium(e.g:the cell supernatant of HUVEC cells was used as SKOV3/PM4 condition medium)and to establish the condition cultrue cell system and the co-culture cell system of the two cell lines. In the condition cultrue cell system, The morphological changes of cells were observed by HE staining to calculate the mitotic index. The ultrastructural changes of the two cells were observed by transmission electron microscopy(TEM). The growth curve of the cells was determined by methyl thiazolyl tetrazolium (MTT) assay and flow cytometry was used to analyzed the cell cycles.In the co-culture cell system, the interaction of the two cells were detected by laser scanning confocal microscope(LSCM). The expression of matrix metalloproteinase-2(MMP-2) and matrix metalloproteinase-9 (MMP-9) were detected by gelatin zymography. Results Compared with the single culture SKOV3/PM4, the cells which cultured in HUVEC condition medium showed the increase of pseudopodia and nuclear division,the mitotic index respectively were [(4.8 ± 0.8)%,(11.2 ± 0.3)%;P<0.05]. The growth rate was significantly increased. In cell cycles, it showed the declined cell ratio of G0/G1 phase, respectively[(69.4±3.6)%, (48.4±4.6)%;P<0.05] and the raised cell ratio of G2/M phase, respectively [(5.2±1.6)%, (24.9±2.2)%;P<0.05]. Compared with the single culture HUVEC,the cells which cultured in SKOV3/PM4 condition medium showed the significant morphological change and vacuolization in the cytoplasm, Nuclear division was increased and the mitotic index respectively were [(2.7±0.5)%, (5.7±0.6)%;P<0.05]. The growth rate was slightly declined. In cell cycles, it showed the raised cell ratio in G0/G1 phase, respectively [(51.4 ± 2.2)%,(79.0 ± 4.1)%;P<0.05] and the declined cell ratio in G2/M phase, respectively [(19.1±1.2)%, (3.3±0.5)%;P<0.05]. After co-culture for 48 hours, spontaneous fusion between SKOV3/PM4 and HUVEC cell was observed by the laser confocal microscope. Gelatin zymography assay showed that MMP-2 was not expressed in HUVEC cells, low-expressed in SKOV3/PM4 cells and high-expressed in the co-culture SKOV3/PM4+HUVEC cells. The expression of MMP-2 in co-culture SKOV3/PM4+HUVEC cells and SKOV3/PM4 cells respectively were 1 885 ± 84 and 1 209 ± 114 (P<0.05). But there were no MMP-9 expression in HUVEC cells, SKOV3/PM4 cells, and the co-culture SKOV3/PM4+HUVEC. Conclusion The characteristics of SKOV3/PM4 and HUVEC show significant changes after condition culture and co-culture, it may involve in the microenvironment of the cells and the intercellular crosstalk pathway.

Factors affecting effectiveness of team learning of college students and their relationship / 中华行为医学与脑科学杂志

Objective To explore the key antecedent variables of team effectiveness and their correlation.Methods The stratified cluster random sampling was conducted to recruit 133 college students from a university in Fujian Province.Results (1) Communication and back up behaviors were positive predictors of team grade (β =0.645,0.315,P＜0.01)and satisfaction degree of team members (β=0.305,0.309,P＜0.01).Free riding had negative influence to the satisfaction of team members (β=-0.392,P＜0.01).(2)Accuracy of tusk cognition and tusk concern were positively related to team performance (β=0.513,0.470,P＜0.01),and accuracy of tusk cognition and team efficacy were predictors of satisfaction of team members (β=0.502,0.373,P＜0.01).(3)Team process variables mediated the relationship between team mentalmodel and tear effectiveness.Conclusion Antecedent variables include team process variables and team mental model.Between team mental model and team effectiveness,the team process variables play a mediating role.

Analysis of the effect of human lymphatic endothelial cells on proteins secreted by epithelial ovarian cancer cells with lymph node-oriented metastasis / 中国肿瘤临床

Objective:This study aimed to investigate the effect of human lymphatic endothelial cells (HLECs) on proteins secreted by epithelial ovarian cancer (EOC) cells SKOV3-pm4 with highly directional lymphatic metastasis. Methods:The supernatants of the four groups of cultured cells (A, SKOV3;B, SKOV3+HLEC;C, SKOV3-PM4;and D, SKOV3-PM4+HLEC) were collected. The proteins of these cells were detected by antibody arrays and iTRAQ-2D-LC-MALDI-TOF/TOF/MS. The screened significantly differential proteins were further analyzed by bioinformatics and validated in the human serum and cell culture medium by ELISA. Results:Progranulin (GRN) and vascular endothelial growth factor A (VEGF-A) were upregulated between groups C and A. In addition, insulin-like growth factor binding protein-7 (IGFBP-7) and secreted protein acid rich in cysteine (SPARC) were downregulated between groups D and C. Comprehensive bioinformatics analysis revealed that IGFBP7 interacted with VEGFA. VEGF exhibited the highest expression in ovarian cancer and IGFBP7 exhibited the lowest expression compared with the serum of the normal control group. Statistically significant differences were observed between the two substances. Conclusion:The HLEC microenvironment is closely associated with directional metastasis in lymph nodes with differential proteins, including matricellular proteins and adhesion factors. In particular, the upregulation of VEGFA and GRN and the downregulation of SPARC and IGFBP7 were closely associated with the directional metastasis of EOC cells in lymph nodes.

Research on EV71 infection complicated with neurogenic pulmonary edema / 国际儿科学杂志

Enterovirus 71(EV71) infection complicated with neurogenic pulmonary edema (NPE) is an important complication and the main death cause of hand-foot-mouth disease in children. The exact pathogenesis has not yet fully understood. Peripheral sympathetic nervous system hyperexcitability has been recognized, but its central positioning and machanisms are debatable. In recent years, people emphasized the release of cytokines caused by the brainstem injury and/or systemic inflammatory response also plays a very important role in the formation of NPE.The interventions at present are mainly symptomatic treatment in the nervous system involvement, respiratory and circulatory failure. Vaccines are still in the experimental phase of exploration. There is a certain distance from clinical application.

Effects of intra-bone marrow injection of donor bone marrow cells in combination with low dose radiation on the immunologic reaction of composite tissue allotransplantation in rats / 中华器官移植杂志

Objective To study the effects of intra-bone marrow injection of donor bone marrow cells in combination with low dose radiation on the immunologic reaction of composite tissue allotransplantation.Methods The inbred SD rats were chosen as donors and inbred Wistar rats as recipients. Overall 40 recipients were classified into 4 groups randomly after allogeneic leg transplantation: group A received transplantation only; group B irradiation in the sublethal level (4.5 Gy?2 at a 4-h interval) and fludarabine (50 mg/kg, i.p.); group C, bone marrow cells were directly injected into the intra-bone marrow cavity of the recipients; group D, using a combination of the injection of fludarabine (50 mg/kg, i.p.), irradiation (4.5 Gy?2, at a 4-h interval) and injection of donor bone marrow cells. The rejection of grafts was observed. 120 days after induction of tolerance the mixed lymphocyte reaction (MIR) and skin grafting were examined to confirm tolerance status. To determine graft-versus-host disease (GVHD), rats in tolerance status were also histologically examined. Results As compared with other groups, mean rejection time and mean survival time of limb allografts were prolonged obviously in group D. Donor-specific tolerance was confirmed in all limb allograft recipients in group D by skin grafting and by MLR, and no signs of GVHD were also histologically examined. Conclusion Using a combination of injection of fludarabine, irradiation in the sublethal level and donor bone marrow cells, we have induced donor-specific immunological tolerance in allogeneic limb transplantation in rats without using any immnosuppressants after the operation.

One case report of double hand allograft / 中华创伤骨科杂志

Objective To evalute the feasibility of a double hand allogroft to reconstuct the limbs'function. Methods A brain dead donor was chosen under the test of blood group in ABO and Rh,HLA match,PRA and the mixed leukocyte culture.The level of the transplantated hands was 5 cm proximal to the wrists The procedure of the allotransplantation was similar to that of the autologous replantation.The immunosuppressants were taken two days before the operation,in the operation and after the operation. The circulation,the vital signs and the rejection were under the supervision. Results The progress was satisfactory. No rejection was found postoperation.After 10 weeks the callus in both ends of radius and ulna was obvious.After 5 months sensation recovered.After 7 months the internal fixation(plates and screws) was removed and tenolysis was done. Now the recipient could use engrafted hands to wash face,make the bed,wear clothes,brush teeth,peel oranges,eat with a spoon,tie the shoelace slowly,pick up a telephoneor and use the conroller of Television. Conclusions With the immunosuppressive agents,the double hands allograft can survive.The growth rate of bone and nerve is faster than that of autologous replantation.

Indoor Air Pollution and Its Health Effects in Newly Decorated Rooms / 环境与健康杂志

Objective To explore the indoor air pollution and its health effects in newly decorated rooms. Methods Indoor air sampling was conducted in rooms of 13 newly decorated apartments during the period from the 1st day to the 4th day, the 2nd month and the 6th month after the decoration. The levels of formaldehyde, benzene, o-dimthylbenzene, m-dimethylbenzene, para-dimethylbenzene in air samples were analyzed. An epidemiological investigation on the adverse response of eye and respiratory system was carried out among the family members before and after decoration. Results The levels of formaldehyde, benzene and dimethylbenzene in indoor air exceeded ther related standards more than 2.92 times, 7.4-11.3 times, 58.2-87.1 times respectively, and showed a decreasing trend with the prolongation of time after decoration. The positive rates of shedding tears and sneezing, branchitis or cough, asthma, dizziness among family members were 0%, 3.0%, 3.0%, 0% respectirely before decoration and rose to 24.2%, 7.6%, 6.1%, 7.6% respectively after decoration. Conclusion The indoor decoration resulted in the indoor air pollution by formaldehyde and benzene compounds, the contamination degree showed a positive correlation with the degree of complicated decoration and was associated with the quality of decorative materials. Indoor decoration also resulted in the increasing positive rates of adverse response of eye and respiratory system.

Effects of Air Quality on the Respiratory System of Pupils / 环境与健康杂志

Objective To study the effects of air quality on the the respiratory system of pupils. Methods Based on the routine monitoring data of Nanning City from Nanning environmental monitor station , all pupils from three elementary schools which located in heavy polluted area, middle polluted area and light polluted area respectively were investigated. The atmosphere quality was assessed with Shanghai Atmosphere Environmental Index. The illness data were collected by epidemiological investigation, questionnaire and physical examination. Chi-square test and Logistic regression were employed in the statistic analysis. Results The morbidity of bronchitis, pneumonia, asthma, rhinitis, etc. in pupils lived in heavy polluted area was higher than that in light polluted area (P