RESUMO
AIM To establish an HPLC method for the simultaneous content determination of arctiin,arctigenin,forsythoside A,narirutin,naringin,rhoifolin,prim-O-glucosylcimifugin and 5-O-methylvisammioside in Wuli Huichun Pills (Saposhnikoviae Radix,Arctii Fructus,Forsythiae Fructus,etc.).METHODS The analysis of 70% methanol extract of this drug was performed on a 25 ℃ thermostatic Agilent TC-C18 column (4.6 mm ×250 mm,5 μm),with the mobile phase comprising of methanol-0.4% glacial acetic acid flowing at 0.9 mL/min in a gradient elution manner.RESULTS Eight constituents showed good linear relationships within their own ranges (r > 0.999 0),whose average recoveries were 96.55%-99.50% with the RSDs of 0.54%-1.72%.CONCLUSION This accurate,reliable and reproducible method can be used for the quality control of Wuli Huichun Pills.
RESUMO
AIM To establish an HPLC-ELSD method for the simultaneous content determination of euscaphic acid,tormentic acid,crategolic acid,corosolic acid,ophiopogonin D,ophiopogonin D',methylophiopogonanone A and methylophiopogonanone B in Shenmai Zhisou Syrup (Eriobotryae Folium,Ophiopogonis Radix,Glehniae Radix,etc.).METHODS The analysis of methanol extract of this drug was performed on a 35 ℃ Alltima C18 column (4.6 mm × 250 mm,5 μm),with the mobile phase comprising of (acetonitrile-methanol)-0.5% ammonium acetate flowing at 0.7 mL/min in a gradient elution manner.RESULTS Eight constituents showed good linear relationships within their own ranges (r > 0.999 0),whose average recoveries were 96.85%-99.43% with the RSDs of 0.57%-1.33%.CONCLUSION This simple and accurate method can be used for the rapid quality control of Shenmai Zhisou Syrup.