Exploration of Curriculum Educational Reform on Higher Vocational Traditional Rehabilitation Treatment Technology / 中国康复理论与实践

@#In view of the different characteristics of the course content,the author used the different teaching methods and measure,and discussed the course teaching reform in higher vocational traditional rehabilitation treatment technology.

Exploration of Curriculum Educational Reform on Higher Vocational Traditional Rehabilitation Treatment Technology / 中国康复理论与实践

@#In view of the different characteristics of the course content,the author used the different teaching methods and measure,and discussed the course teaching reform in higher vocational traditional rehabilitation treatment technology.

The breeding and culture condition optimization of a high-biomass, selenium-enriched yeast strain / 生物工程学报

The yeast fusant ZFF-28, which is high in biomass production and rich in selenium, was constructed after mutagenesis and protoplasts fusion between yeast strains. The total selenium content of ZFF-28 is 1.8 and 1.0 times higher than that of the parental strains Saccharomyces cerevisiae ZY-67 and Saccharomyces kluyveri SZY-198 respectively. Using single factor tests and a L16(4(3) x 2(1)) orthogonal design, the cultivation conditions was optimized as: 50mL culture in 250mL shake flasks in molasses containing 6% sugar and 60microg/mL Se at 28 degree C for 25h at 220 r/min, with the initial pH adjusted to 6.0 - 6.5. Under the optimized conditions, the biomass (dry weight) reached 8.2g/L and the Se content of the cells reached 2050microg/g, with organic and inorganic Se contents being 91% and 9% respectively.