Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Adicionar filtros








Intervalo de ano
1.
Chinese Journal of Stomatology ; (12): 712-715, 2007.
Artigo em Chinês | WPRIM | ID: wpr-359634

RESUMO

<p><b>OBJECTIVE</b>To establish a quick and reliable method to identify Streptococcus oligofermentans, a new species of oral streptococci.</p><p><b>METHODS</b>With two-step PCR, a pair of the 16S rDNA-specific primers of Streptococcus oligofermentans and a pair of primers of lactate oxidase gene (lox) were used to amplify the gene fragments from the genomic DNAs of 11 strains consisting of 9 species of the pure culture of oral streptococci. Pooled plaque samples from 9 caries-free volunteers were cultured on a selective medium of MSA with erythromycin and tentative strains of Streptococcus oligofermentans were isolated. The isolates were further identified by the two-step PCR and finally confirmed by 16S rDNA sequence analysis.</p><p><b>RESULTS</b>With the two-step PCR, the two gene fragments were only amplified from the three identified strains of Streptococcus oligofermentans, but not the rest of 8 strains of oral streptococci. Isolates from the dental plaque of caries-free volunteers were identified as Streptococcus oligofermentans by PCR and then further confirmed by 16S rDNA sequence homology analysis.</p><p><b>CONCLUSIONS</b>Streptococcus oligofermentans could be identified by the two-step PCR approach with the specific 16S rDNA primers and lactate oxidase gene primers.</p>


Assuntos
Técnicas de Tipagem Bacteriana , Métodos , Primers do DNA , Genética , DNA Bacteriano , Genética , DNA Ribossômico , Genética , Oxigenases de Função Mista , Genética , Reação em Cadeia da Polimerase , Métodos , RNA Ribossômico 16S , Genética , Análise de Sequência de DNA , Streptococcus , Classificação , Genética
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA