RESUMO
Objective:To study the relationship between TCM syndrome type of chronic diarrhea, chronic diarrhea and negative colonoscopy terminal ileum lesions by colonoscopy.Methods:104 cases in our hospital underwent colonoscopy patients (each patients colonoscopy pushed to the terminal ileum is more than or equal to 20 cm) were divided into control group and observation group by examination results, each with 52 cases. The patients in the control group had no chronic diarrhea and the observation group had negative chronic diarrhea. The observation group was given the lines of traditional Chinese medicine syndrome differentiation typing, and chronic diarrhea associated with ileum end lesions were given death drop spirit combined micro ecological preparation, and then review the lesions after 15 days.Results: In the observation group, 1 case was TCM sub type of damp heat in spleen and stomach (1.92%), 2 cases were spleen deficient deficiency (3.84%), 3 cases were liver stagnation (5.77%), 4 cases were deficiency of spleen and liver (7.69%), 8 cases were cold-dampness disturbing spleen (15.38%), 9 patients were liver stagnation and spleen deficiency (17.31%), 11 cases were spleen and kidney deficiency (21.15%), and 14 cases were spleen wet trapped (26.92%). There were 37 cases (71.15%) in the observation group of patients with ileal mucosal edema, congestion, erosion and ulceration and 12 cases in the control group (23.08%). The differences between the two groups were statistically significant (x2=24.12,P<0.05). There were 38 cases in the observation group and 7 cases in the control group with ileal follicular hyperplasia. There were 18 cases were mid hyperplasia in the observation group and 8 cases in the control group. The differences were significant(x2=37.64,x2=21.31;P<0.050). The size of patients with hyperplasia was 4.5mm in the observation group and 1.2mm in the control group. The differences between the two groups were statistically significant (Z=13.05,P<0.05). 46 cases of chronic diarrhea symptoms disappeared after treatment and 26 cases of colonoscopy terminal ileum lesions were improved. The differences before and after treatment were statistically significant (70.27%).Conclusion: Patients with chronic diarrhea syndrome are found lesions in terminal ileum. The symptom would disappear or be greatly improved after medicine treatment. It can be promoted as a reference standard of clinical medication.
RESUMO
<p><b>OBJECTIVE</b>To establish a method for determination of geniposide in Beagle dogs plasma by high performance liquid chromatography (HPLC), and study the pharmacokinetics and bioavailability of geniposide in Beagle dogs after oral administration Xingnaojing.</p><p><b>METHOD</b>To determine the geniposide in Beagle dogs plasma by HPLC after oral administration or intravenous injection Xingnaojing, and the pharmacokinetic parameters were calculated by the software of Kinetica.</p><p><b>RESULT</b>The good linearity range of geniposide was 1.24 - 158.88 mg x L(-1). The main pharmacokinetic parameters after oral administration was as follows: Cmax (11.8 +/- 0.6) mg x L(-1), Tmax (52.0 +/- 4.5) min, AUC(1280.8 +/- 172.0) mg x min x L(-1), MRT(118.7 +/- 25.4) min, and these parameters after intravenous injection was follows: Cmax 107.4 +/- 6.3) mg x L(-1), AUC(7930.1 +/- 670.0) mg x min x L(-1), MRT(92.4 +/- 5.1) min. The bioavailability of geniposide in Beagle dogs after oral administration Xingnaojing was (6.46 +/- 0.87)%.</p><p><b>CONCLUSION</b>The HPLC method had good applicability. The extract recovery, method recovery, intra-day precision and inter-day precision of the method were all met the requirements. The stability in conditions of room temperature and freeze-thaw cycle was good. The results indicated that the oral administration bioavailability of geniposide was in low degree.</p>