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Objective To evaluate the effect of self -efficacy training on hemiplegic patients after ischemic stroke. Methods Sixty cases were randomly divided into control group (n =30 )and self -efficacy training group (n =30).The self -efficacy group received rehabilitation training according to the self -efficacy theory (5 times /week, 40 min /per time,1 0 weeks of intervention).The movement and balance function were evaluated using Fugl -Meyer score (FMA)and the activities of daily living were evaluated by the task analysis scale and Barthel index.The self -efficacy level and quality of life were evaluated by the general self -efficacy scale (GSES)and medical outcomes study (SF -36).Results The scores of FMA,Barthel,SF -36 and GSES had no significant differences between the two groups before training (P >0.05 )and all indexes improved after training.Six scores of Barthel and all scores of FMA,SF -36 and GSES of self -efficacy group were significantly higher than those of control group after training (all P <0.05 ).Conclusion Self -efficacy application could improve the motor function on the rehabilitation of hemiplegic patients after ischemic stroke.
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<p><b>OBJECTIVE</b>To investigate the effects of α3 neuronal nicotinic acetylcholine receptor (nAChR) on apoptosis and p38 signal transduction pathway in SH-SY5Y cells and to assess the roles of α3 nAChR in the pathogenesis of Alzheimer's disease (AD).</p><p><b>METHODS</b>The levels of α3 nAChR mRNA and protein were measured by real-time PCR and Western blot, respectively, in SH-SY5Y cells transfected with α3 nAChR siRNA. The mRNA level of bcl-2 and bax was measured by the real-time PCR. The siRNA transfected SH-SY5Y cells and control were then treated with 10 µmol/L Aβ25-35 for another 48 h, and the change in apoptotic rate and the levels of p-p38 and p38 were measured by flow cytometry and Western blot. Subsequently these SH-SY5Y cells were exposed to a blocker of p38 protein, and the apoptotic rate was measured again.</p><p><b>RESULTS</b>Compared to the controls, the expression of α3 nAChR at mRNA and protein levels in the SH-SY5Y cells transfected with α3 nAChR siRNA decreased by 95% and 86%, respectively; the mRNA levels of bax increased 2.11 times and that for bcl-2 decreased 0.53 times. The apoptotic rate was unaffected (3.40% ± 0.20%); but it increased after Aβ25-35 treatment (24.52% ± 1.59%); the level of p-p38 protein also increased by 178% in the α3 nAChR inhibited cells treated with Aβ25-35. Compared to controls, the Aβ25-35-treated SH-SY5Y cells and the Aβ25-35-treated and siRNA-transfected cells both showed a reduction in apoptosis after treatment with p38 blocker, especially in the former.</p><p><b>CONCLUSION</b>The siRNA silencing of α3 nAChR mRNA may enhance the effect of Aβ25-35 on the cell apoptosis by increasing the levels of p38 protein and bax mRNA and decreasing the level of bcl-2 mRNA, which may play a role in the pathogenesis of AD.</p>
Assuntos
Humanos , Doença de Alzheimer , Peptídeos beta-Amiloides , Metabolismo , Apoptose , Linhagem Celular Tumoral , Inativação Gênica , Neuroblastoma , Metabolismo , Patologia , Fragmentos de Peptídeos , Metabolismo , Proteínas Proto-Oncogênicas c-bcl-2 , Genética , Metabolismo , RNA Mensageiro , Metabolismo , RNA Interferente Pequeno , Genética , Receptores Nicotínicos , Genética , Metabolismo , Transdução de Sinais , Transfecção , Proteína X Associada a bcl-2 , Genética , Metabolismo , Proteínas Quinases p38 Ativadas por Mitógeno , MetabolismoRESUMO
<p><b>OBJECTIVE</b>To compare clinical therapeutic effects of double moxibustion at Yifeng (TE 17) combined with electroacupuncture. and simple electroacupuncture on facial paralysis.</p><p><b>METHODS</b>Eighty-seven cases of facial paralysis were randomly divided into a treatment group (n = 47) treated by double moxibustion at Yifeng (TE 17) combined with electroacupuncture, and a control group (n = 40) treated by simple electroacupuncture. Their therapeutic effects were investigated after 30 days.</p><p><b>RESULTS</b>The total effective rate was 100.0% in treatment group and 87.5% in the control group with a significant difference between the two groups (P < 0.05).</p><p><b>CONCLUSION</b>Double moxibustion at Yifeng (TE 17) combined with electroacupuncture in clinical therapeutic effect on peripheral facial paralysis is superior to simple electroacupuncture.</p>