Determination of scopolamine and atropine in Flos Daturae by RP-HPLC / 中国中药杂志

<p><b>OBJECTIVE</b>To develop a quantitative analytical procedure of scopolamine and atropine in Flos Daturae using RP-HPLC.</p><p><b>METHOD</b>The two alkaloids were separated on a Hypersil BDS C18 column (4.6 mm x 250 mm, 5 microm) with a mobile phase of 0.02 mol x L(-1) sodium acetate buffer (containing 0.02% triethanolamine and the pH was adjusted to 6.0 with acetic acid)-methanol (60:40) and a detection wavelength of 215 nm. The flow rate was 1.0 mL x min(-1) and the column temperature was maintained at room temperature.</p><p><b>RESULT</b>The mean recovery was (99.6 +/- 1.8)% for scopolamine and (100.4 +/- 1.5)% for atropine.</p><p><b>CONCLUSION</b>This method was simple, accurate and sensitive.</p>