Exploration on Determination and Quantity Transfer of Index Components in Benchmark Samples of Erdongtang / 中国实验方剂学杂志

ObjectiveTo establish the determination for index components in benchmark samples of Erdongtang， and clarify the content and transfer rate rages of index components in 15 batches of benchmark samples， and to explore the quantity transfer of index components of decoction pieces to benchmark samples. MethodFifteen batches of benchmark samples were prepared， the contents of mangiferin， baicalin and glycyrrhizic acid were determined by high performance liquid chromatography （HPLC）-diode array detector （DAD）， the mobile phase was acetonitrile （A）-0.1% formic acid aqueous solution （B） for gradient elution （0-10 min， 10%-17%A； 10-25 min， 17%-19%A； 25-28 min， 19%-25%A； 28-45 min， 25%-33%A； 45-46 min， 33%-45%A； 46-60 min， 45%-55%A）， detection wavelength was set at 254 nm. Contents of timosaponin BⅡ and the sum of protoneodioscin and protodioscin were determined by HPLC-evaporative light scattering detector （ELSD）， the mobile phase was acetonitrile （A）-water （B） for gradient elution （0-20 min， 24%A； 20-25 min， 24%-27%A； 25-33 min， 27%-28%A； 33-36 min， 28%-90%A； 36-41 min， 90%-24%A）. ResultThe methodological verification of the established method was good， which could be used for determination of five index components in benchmark samples. The content ranges of mangiferin， baicalin， glycyrrhizic acid， timosaponin BⅡ， and the sum of protoneodioscin and protodioscin in 15 batches of benchmark samples of Erdongtang were 0.14%-0.23%， 2.40%-3.37%， 0.07%-0.44%， 0.43%-0.95%， and 0.15%-0.47%， the transfer rate ranges of them were 33.90%-52.15%， 84.46%-105.61%， 22.59%-93.86%， 38.07%-61.43%， and 53.28%-96.11%， respectively. ConclusionThe consistencies of transfer rate of mangiferin， baicalin， timosaponin BⅡ and the sum of protoneodioscin and protodioscin （except glycyrrhizic acid） between decoction pieces and benchmark samples of Erdongtang are good， indicates that the transfer rates of 4 index components are stable during the preparation process of benchmark samples， which can provide data support for research and development of the compound preparation of this formula.

Quality evaluation methods for standard decoction of Nelumbinis Folium / 中国中药杂志

The quality evaluation method for standard decoction of Chinese herbal slices is the basis for the quality evaluation of granules and preparations of classical formula(decoction)of traditional Chinese medicine. This study aimed to establish a method for the determination of quercetin-3-O-glucuronic acid in Nelumbinis Folium(NF)and its standard decoction, so as to provide reference for the quality control of NF and its standard decoction. Fifteen batches of representative NF were collected to prepare standard decoction, and the parameters of dry extract rate, transfer rate of index component, and pH value were calculated. HPLC was used to establish the content determination method for quercetin-3-O-glucuronic acid in NF and its standard decoction. The concentration range of quercetin-3-O-glucuronic acid in the standard decoction of NF was 1.09-3.06 g·L~(-1), while the concentration range of nuciferine was 0.01-0.17 g·L~(-1). The average extraction rate of NF standard decoction was(14.4±2.6)%, the average transfer rate of quercetin-3-O-glucuronic acid was(70.7±18.6)%, and the average transfer rate of nuciferine was(9.6±5.4)%. Compared with Nuciferine, quercetin-3-O-glucuronic acid had a high content and stable transfer rate in standard decoction, and was recommended to be the quality control marker for NF and its standard decoction. This paper establishes a quality evaluation method for NF standard decoction, and can provide reference for the quality control of all preparations derived from NF and its decoction.

Study on five efficacy components, geoherbalism of Cistanche deserticola from genuine producing area in different collecting seasons / 中草药

Objective To investigate the contents of major five functional components (echinacoside, verbascoside, galactitol, betaine, soluble polysaccharide, and extractums) in Cistanche deserticola harvested in spring and autumn from genuine producing area in Alax Banner of Inner Mongolia. Methods HPLC-UV was applied to measuring the contents of echinacoside and verbascoside. HPLC-ELSD was used for determining the contents of galactitol and betaine. UV-VIS was utilized for analyzing the content of soluble polysaccharide. Results The index components of two samples' harvested in spring and autumn were all up to the standard of Chinese Pharmacopoeia. The samples harvested in spring contain 12.21 mg/g, which was twice of the autumn samples and fourfold of the standard of Chinese Pharmacopoeia. Based on verbascoside and betaine, the content of spring samples was significantly higher than the autumn samples, which was up to thirtyfold and it had great fluctuation among samples; Based on soluble polysaccharide, the content of spring and autumn samples were all at a high level, especially autumn samples was up to 13.7%; Based on galactitol and extractums, the content of autumn samples was significantly higher than the spring samples. Conclusion The content of verbascoside in C. deserticola has great fluctuation among samples. C. deserticola, that is rich in galactitol and soluble polysaccharide that authentic inner quality characteristics are "Glossy, Heavy, Fleshy, Quality soft, Sweet". Nevertheless, the ample galactitol and soluble polysaccharide is the material basis of quality formation, it is more reasonable to add the soluble polysaccharide as one of the index component to evaluate its quality characteristics. The standard of Chinese Pharmacopoeia points out the moisture limit of succulent herb is 10% may not be reasonable. The further improvement of the standard of C. deserticola's quality or separation of C. deserticola was discussed in this manuscript.

An improved method of quantitative assessment of regional cerebral blood flows by perfusion CT at the general infusion rate / 中华放射学杂志

Objective To improve the conventional method of quantitative assessment of regional cerebral blood flows(rCBF)by a perfusion CT study based on maximal slope model at the general infusion rate(