Isolation of endophytes from Panax quinquefolium and screening and identification of strains with antagonistic activity / 中草药

Objective: To study the resource situation of endophytes in Panax quinquefolium of Jilin province and to select the endophytes with antagonistic effect on pathogenic fungi of P. quinquefolium. Methods: The strains with the antagonistic activity were screened by applying mixed strains method during preliminary screening and adopting the antagonism method of fermentation liquor in re-screening; Besides, the selected endogenous strains were identified via 16 S rDNA and ITS method. Results: Thirty-four endogenous strains were isolated from P. quinquefolium. After preliminary screening and re-screening, six strains with good antagonistic effect on pathogenic fungi of P. Quinquefolium were selected: B3, B11, B17, B23, B24, and F10. Among them, B3 showed an inhibitory effect on seven pathogenic fungi; F10 had a good inhibitory effect on Cylindrocarpon destructans and Alternaria panax, with an inhibitory zone diameter over 35 mm; According to the identification, B3 and F10 were Bacillus methylotrophicus and Penicillium citreonigrum respectively. Conclusion: Endophytes in P. quinquefolium are diversified and there also exist strains with high antagonistic activity, which can be a good source for biocontrol bacterium and fungus of P. quinquefolium diseases.

Treatment of AIDS patients with Chinese medicinal herbs qudu zengning capsule / 中华实验和临床病毒学杂志

<p><b>OBJECTIVE</b>To evaluate the therapeutic effect of QuDu ZengNing Capsule on AIDS.</p><p><b>METHODS</b>QuDu ZengNing Capsule is a capsule containing extract from 4 Chinese medicinal herbs. Totally 1,000 AIDS patients were treated, among them 60 patients were clinically observed weekly. Blood routine tests, liver, heart and kidney function, X-ray, CD4, CD8 cells were examined before and after treatment at 1, 3, 6 month. The patients were treated with 4 capsules t.i.d for 6 months.</p><p><b>RESULTS</b>The symptoms were improved in most of the patients, the CD4 cells increased from 115.0 to 295.2/ul and the viral load (RNA copies/ml) in most patients reduced markedly or maintained at the same level.</p><p><b>CONCLUSION</b>These data indicated that QuDu ZengNing Capsule was effective for treatment of AIDS patients.</p>