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Objective To establish a mouse model of hepatocyte-specific TM6SF2 knockout, and to investigate the role of TM6SF2 in the development of nonalcoholic fatty liver disease (NAFLD). Methods The CRISPR/Cas9 technique and the Cre/LoxP strategy were used to establish a stable mouse model of hepatocyte-specific TM6SF2 knockout. The mice with hepatocyte-specific TM6SF2 knockout and the control mice were given a normal diet or a high-fat diet (HFD) for 16 weeks, and related indices were measured, including general status (body weight and liver weight), glucose metabolic indices (fasting blood glucose and insulin), and lipid metabolism (plasma triglyceride, cholesterol, and liver triglyceride). The t -test was used for comparison of normally distributed continuous data between two groups. Results Under the condition of HFD, compared with the control mice, the mice with hepatocyte-specific TM6SF2 knockout had significantly higher liver weight (2.235±0.175 g vs 1.258±0.106 g, t =4.789, P 0.05). Under the condition of HFD, there were no significant differences in the levels of plasma triglyceride and cholesterol between the mice with hepatocyte-specific TM6SF2 knockout and the control group ( P > 0.05), while the mice with hepatocyte-specific TM6SF2 knockout had a significant increase in the level of liver triglyceride compared with the control mice (23.969±0.978 mg/g vs 18.229±1.633 mg/g, t =3.015, P =0.024). Conclusion Hepatocyte-specific knockout of TM6SF2 can aggravate liver lipid accumulation and liver injury in mice with NAFLD.
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Objective To investigate the clinical efficacy of pedicle screw fixation and injured vertebral bone graft and facet joint grafting in the treatment of thoracolumbar fractures with spinal cord compression.Methods A prospective controlled study was conducted to analyze the clinical data of 60 patients with thoracolumbar fractures complicated with spinal cord compression from January 2015 to April 2017 in Shenzhen Pingle Orthopedic Hospital.The patients were divided into two groups in random number table method.Each group of 30 cases.The patients in the observation group were treated with pedicle screw fixation and injured vertebral bone graft and facet joint grafting in the observation group.The patients in the control group were treated with traditional posterior approach root screw fixation and transverse bone graft was performed.Tactile,vertebral height,motion and Cobb's angle of the two groups were evaluated preoperatively and one month after operation.The patients were followed up by phone for six months.The ASIA score of the American Spinal Injury Association was used to evaluate the neurological function recovery of the two groups to observe the curative effect.Using SPSS 13.0 statistical software analysis,count data were expressed as percentage,measurement data were expressed by ((x) ± s),the two groups were compared using t test.Results The tactile sensation,vertebral height,motion and Cobb's angle in observation group were significantly higher than those before operation (59.4 ± 17.3) scores,(58.8 ± 16.6) scores,(55.8 ± 23.6) scores and (54.5° ± 21.4°) scores respectively],in the control group [(75.6 ± 14.9) scores,(72.4 ± 14.5) scores,(76.8 ± 23.3) scores,(76.2° ± 23.5 °)],with statistical differences (P ＜ 0.05).After the observation group was followed up for one month,the neurological function recovery was better than that of the control group (P ＜ 0.05).Conclutions Pedicle screw fixation and injured vertebral bone grafting via percutaneous multifidus muscle interspace can play an important role in the treatment of thoracolumbar fractures with compression of the spinal cord.It can significantly relieve spinal cord compression in patients.
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Objective To investigate the prevalence of thyroid nodules and thyroid dysfunction in southern mountainouss areas of Ningxia.Methods A cross-sectional study was conducted among a representative sample of 10 639 adults in Jingyuan county with a population proportionate sampling method.High-resolution ultrasound was used to examine the thyroid and fasting blood specimens were collected in the morning for measurement of TSH,FT4,FT3.Chi-square test and spearman rank correlation analysis were used for statistical analysis.Results The prevalence of thyroid nodules was 29.08％,the sex-and age-adjusted rate was 27.17％.The prevalence of thyroid nodules was higher in women than in men (32.68％ vs.24.88％,x2=76.029 2,P＜0.001) and age was positively associated with thyroid nodules (r=0.272,P＜0.001).The rate of thyroid dysfunetion,subclinical hypothyroidism,subclinical hyperthyroidism,hypothyroidism,hyperthyroidism were 17.39％,13.00％,0.42％,0.96％,3.01％,respectively.The prevalence of thyroid nodules was higher in abnormal TSH group than in normal TSH group (39.44％ vs.27.24％,x2=95.624 0,P＜0.001).The level of THS,FT3,FT4 in thyroid nodules group differed fromn control group (Z=-9.144,P＜0.001;Z=-6.140,P＜0.001;Z=-1.997,P=0.046).Conclusion The prevalence of thyroid nodules and thyroid dysfunction were higher in southern mountainous areas of Ningxia.The relationship between thyroid nodules and thyroid function needs further research.We should pay attention to the early screening and diagnosis of thyroid nodules in mountainous areas.