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Objective:To explore the application effect of pre-hospital information publicity and education management model in patients with gastrointestinal tumors.Methods:The inpatients with gastrointestinal tumors who made an appointment in the outpatient department of the Affiliated Hospital of Qingdao University were randomly divided into 225 cases in the control group and 226 cases in the experimental group. The control group was treated according to the routine process, the experimental group implemented pre hospital information education and management, and the patients were individually assessed and health education through the official account of WeChat, Heals mobile education system, telephone communication and WeChat group.Results:After the implementation of pre-hospital information management model, the anxiety and depression scores of patients in the experiment group were (55.89 ± 15.53) and (56.19 ± 17.87), which were significantly better than those in the control group, (60.84 ± 15.42) and (62.28 ± 19.67) ( t = 3.40, 3.43, both P<0.01). The awareness rate of admission related knowledge (74% to 93%) and satisfaction with pre-hospital publicity and education management in the experimental group were also higher than control group ( χ2 values were 5.84-20.28, all P<0.05). The hospitalization rate of patients in the experimental group was 91.15%(206/226), which was significantly higher than that in thecontrol group, 81.78%(184/225), the difference was significant ( χ2 = 8.47, P<0.01). The secondary hospitalization rate in the experimental group was 8.41%(19/226), which was significantly lower than that in the control group, 19.11% (43/225)( χ2 = 10.90, P<0.01). Conclusion:The pre-hospital information publicity and education management model can improve the awareness rate of patients′ admission related knowledge, improve patients′ satisfaction with pre hospital publicity and education management, reduce the secondary hospitalization rate and shorten the average preoperative hospital stay, which is worthy of further promotion and application in clinic.
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Objective:To provide microsurgical anatomy data in the course, branch, distribution, arterial network profile of the submental artery and the range of the flap excision in submental flap transplantation.Methods:From March, 2015 to March, 2020, a total of 36 head and neck cast specimens were studied. Acrylic-butadience-styrene plastic (ABS) filler were perfused into the external carotid artery to make cast specimens. The course, branching, distribution and the arterial framework of the submental artery under a surgical microscope were investigated.Results:The submental artery originated from the facial artery before reaching the lower edge of the mandible (1.50±0.50) cm, with a diameter of (1.50±0.85) (0.6-2.3) mm. The main trunk of submental artery was (5.5±0.5) cm in length, which ran forward along the lower edge of the mandible and branched out (9.0±3.0) (7-13) branches with diameters between 0.1-0.5 mm, and mainly distributed to skin and superficial fascia of the submental area. The main trunk of submental artery divided into ascending, horizontal and descending branches about 3.0 cm of the midline of the mandible. The ascending branch went upwards over the lower edge of the mandible and joined up with the lower labial arch or participated in the formation of the lower labial arch; the horizontal branch divided into several branches and joined up with the branches from the opposite side; the descending branch branched posteriorly and inferiorly, joined up with branches of lingual artery and superior thyroid artery. The branches of the submental artery and the branches of the peripheral arteries were joined up in the submental area to form the submental artery network. The diameter of the vessels in the network ranged 0.1-0.2 mm. The arterial network was built in the form of 1 to 3 layers, and the area of main network was about 7.0 cm×5.0 cm.Conclusion:The submental artery has a long trunk, many branches and abundant anastomoses between the branches, forming a dense submental artery network, which provides sufficient pedicle length, rich blood supply and cutting area for submental flap. The flap can be transplanted free or transposed. The best location of submental flap is near the midline of arterial network, and the appropriate area is 7.0 cm×5.0 cm.
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Objective To investigate the effect of skull acupuncture on cerebral infarct volume and plasma SOD activity and MDA and NO contents. Method Forty-eight male SPF-grade SD rats were randomized into sham operation, model and skull acupuncture groups. A rat model of middle cerebral artery occlusion (MCAO) was made by intraluminal thread occlusion. Rat nerve function was assessed by Zea Longa neurological function scoring. Rat cerebral infarct volume was measured by TTC staining. Plasma superoxide dismutase (SOD) activity and malondialdehyde (MDA) and nitric oxide (NO) contents were measured by colorimetry. Result Rat neurobehavioral score was significantly lower on day 1, 3 and 7 in the model group than in the sham operation group (P<0.01). TTC measurement showed that cerebral infarct volume was significantly smaller in the skull acupuncture group than in the model group (P<0.01). Plasma SOD activity was lower and MDA and NO contents were higher in the model group than in the sham operation group (all P<0.01). Plasma SOD activity increased and MDA and NO contents decreased after skull acupuncture treatment compared with the model group (all P<0.01). Conclusion Skull acupuncture has a protective effect against cerebral ischemia/reperfusion injury and can increase plasma SOD activity and decrease plasma MDA and NO contents in rats with cerebral ischemia/reperfusion injury.
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BACKGROUND:In vitro experiments have confirmed that human umbilical cord mesenchymal stem cells can be induced to differentiate into hepatocyte-like cells, thus which can be considered to function as liver repair. OBJECTIVE:To observe the therapeutic effect of human umbilical cord mesenchymal stem celltransplantation on acute liver injuries in rats through in vivo animal experiments. METHODS:Healthy Sprague-Dawley rats were randomly divided into three groups:normal control group without modeling, celltransplantation group, and PBS group. Rat models of acute liver injury were prepared by 10%CCl4-olive oil solution in the celltransplantation and PBS groups which were fol owed by intraperitoneal injection of 0.5 mL human umbilical cord mesenchymal stem cellsuspension and 0.5 mL PBS, respectively. RESULTS AND CONCLUSION:Hematoxylin-eosin staining showed that pathological changes related to acute liver injury appeared at 24 hours after intraperitoneal injection of CCl 4 . Then, the liver structure recovered at 7 days after celltransplantation, but it did not recover til the 14th day after PBS injection. Compared with the normal control group, serum alanine aminotransferase and aspartate aminotransferase levels were significantly increased in the other two groups (P<0.05-0.01). In the celltransplantation group, the serum alanine aminotransferase and aspartate aminotransferase levels were significantly lower than those in the PBS group at 3 days after treatment (P<0.05-0.01), and recovered normal y after 7 days. cells positive for anti-human nucleoprotein antibody were found in the portal area of liver tissues in the celltransplantation group after 3 days of transplantation, and then cells positive for anti-human albumin antibody appeared after 7 days. These findings indicate that intraperitoneal transplantation of human umbilical cord mesenchymal stem cells can improve liver function and repair injured liver tissues after acute liver injury in rats to some extent.
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The purpose of this study was to clarify the association between P53 and the Bcl-2 family [Bcl-2, Bax, Bcl-xL, Bcl-xS] expression and apoptosis in pancreatic ductal adenocarcinoma [PDAC]. A total of 70 patients with PDAC were studied. The expression of P53 protein in PDAC was assessed using the immunohistochemical method, which categorized the PDAC patients into two groups: group 1: 36 cases with immunonegative P53[-], and group 2: 34 cases with immunopositive P53[+]. The expression of Bcl-2, Bax, Bcl-xL, and Bcl-xS in the 70 PDAC cases was detected by immunohistochemical and Western blotting methods. The apoptotic index [AI] was also measured in these samples by the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling [TUNEL] method. The relation between P53 and the Bcl-2 protein family and apoptosis was then evaluated. Bcl-2 and Bcl-xS expression was significantly associated with P53 [p<0.05]. No clear associations were found among P53, Bax and Bcl-xL expression [p>0.05]. The AI of groups 1 and 2 was 12.1 +/- 2.47 and 8.1 +/- 1.48, respectively [p=0.023]. There was no relationship between AI and Bcl-2, Bax, Bcl-xL and Bcl-xS expression [p>0.05, respectively]. Bcl-2/Bax ratio was significantly associated with AI [p<0.01]. Bcl-2 and Bcl-xS represent significant anti-and proapoptotic proteins, respectively, modulated through a P53-dependent pathway in PDAC, and P53 modulated apoptosis mainly through Bcl-2/Bax ratio