A study on the perceived barriers of community nurses in evidence-based nursing practice / 中国实用护理杂志

Objective:To explore the barrier factors perceived by community nurses in evidence-based nursing practice.Methods:A cross-sectional survey of perceived barriers in 769 community nurses of China was conducted by using the Barriers to Research Utilization Scale from October 2017 to April 2018. In a semi-structured interview, a total of 15 community nurses were selected by purpose sampling for deeply exploring details of perceived barriers, the influencing of barriers and the needed supports for overcoming the barriers in evidence-based practice. The interview data were transcribed to text and analyzed by using thematic analysis in Nvivo 11.0.Results:Community nurses faced barriers including insufficient resources, support and ability of evidence-based practice of community nurses. The results of interview provided the details of these barriers which had negative impacts on community nurses. To overcome these barriers, evidence-based related training, enhanced supporting resources were needed.Conclusions:Community nurses are facing multiple barriers in evidence-based practice. To promote the ability of evidence-based practice of community nurses, it is necessary to strengthen the support from organization, personnel, resources, and improve the ability of community nurses in evidence-based practice.

The current status of the development of nursing clinic in China-- Based on bibliometrics and content analysis / 中国实用护理杂志

Objective To analyze the current status of nursing clinics in China in the past decades in order to provide reference for the development of nursing clinics and advanced nursing practice in the future. Methods Analyzing articles published from 2007 to 2016 from China National Knowledge Infrastructure,Chinese VIP periodical service platform,Wanfang databases by using the bibliometrics and content analysis. Results A total of 184 articles were found by using the key words of"nursing clinic"or"nurse clinic".The increasing trend was obvious after 2013.83.2%(153/184)of the articles describes the nursing clinics were conducted in the hospital.78.3%(144/184)of nursing clinics provided education, guidance and counseling for the clients.87.5%(161/184)of the articles clearly describes the competence of the nurses in the nursing clinics.The majority of the articles reported using non-experimental design. The top subject of nursing clinics research was chronic diseases (54.5%, 85/184), in which diabetes accounts for 70.6%(70/85). Conclusions An increasing trend of development of nursing clinics in China.The urgent need for the development of nursing clinic in primary medical institutions.More kinds of service should be provided for the clients, not only health education, guidance and counseling. To expand the research field of nursing clinics is needed.

Design of standardized cases and its application in evaluation of the ability of assessment / 中国实用护理杂志

Objective To design standardized cases to evaluate the ability of the assessment among nursing students.Methods A set of standardized cases were designed based on an international standardized nursing language as well as a textbook of health assessment and reference.After validity of the set of standardized cases had been tested by the focus group and expert evaluation,the cases were applied to test the ability of the assessment among a total of 57 nursing students.Data were analyzed statistically.Results The average score of evaluation ability of nursing students was 42.5 (15～57).Nursing students got the highest score in psychosocial dimension,accounting for 50.7％ (13.93/27.5).Among five cases,the most difficult case was case 4,the degree of difficulty was 0.36.Conclusions This set of standardized cases can be used to assess the assessment ability among nursing students,and also can be used to evaluate the effects of training.

Construction and identification of a recombinant PRRSV expressing ORF2 of porcine circovirus type 2 / 病毒学报

Porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) are very two important pathogens that have coursed huge economic losses in swine production in worldwide. In this study,a vector pCMV-TJM containing the full-length cDNA clone of PRRSV attenuated strain TJM-F92 was firstly constructed by PCR method. Then a gene sequence containing Afl II/Mlu I e restriction enzyme sites and a transcription regulatory sequence for ORF6 (TRS6) was inserted be- tween ORF7 and 3'UTR, yielding a expression vector pCMV-TJM-TRS. Subsequently, a plasmid pCMV-TJM-Cap was constructed by cloning of PCV2 ORF2 gene into the unique sites Afl II /Mlu I of pCMV- TJM-TRS plasmid DNA. Then three recombinant PRRSV, rTJM, rTJM/TRS and rTJM/Cap, were rescued by transfection of pCMV-TJM, pCMV-TJM-TRS and pCMV-TJM-Cap into Marc-145 cells, respectively,and confirmed by the genome sequence, restriction enzyme digestion, Western Blot and IFA. They all had the molecular markers which was different from the parent virus. The growth characteristics of the rescued viruses were similar to that of parent virus. rTJM/Cap could also express efficiently PCV2 Cap protein in Marc-145 cells. At passage 8, it still had PCV2 ORF2 gene which examined by RT-PCR. It indicated that the full-length cDNA clone of PRRSV attenuated strain TJM-F92 and recombinant PRRSV rTJM/Cap expressing PCV2 Cap protein were successfully constructed. It made an important foundation for studying on the pathogenic mechanisms of PRRSV and PRRSV-PCV2 vaccine in the future.

Application of parametric image processing of contrast-enhanced ultrasound imaging in diagnosis of ovarian masses / 中华全科医师杂志

ObjectiveTo investigate application value of parametric image processing in contrastenhanced ultrasound imaging in diagnosis of ovarian masses. MethodsFifty cases with ovarian masses underwent routine ultrasound and contrast-enhanced ultrasound imaging using a new dedicated parametric image processing software SonoLiver to analyze patterns of vascular formation and blood stream perfusion in the ovarian mass tissues on a digital video recorder in real time, compare their morphological characteristics of time-intensity curve (TIC) and dynamic vascular patterns (DVP) curve, and analyze quantitatively all indicators generated by SonoLiver.ResultsIn ultrasound imaging of the 50 cases, there were 24 cases (86％) displaying mainly blue lowly-enhanced imaging in those with benign masses and 15 cases (68％)displaying mainly red highly-enhanced imaging in those with malignant masses, with statistical significance (P ＜0. 01 ). There was significantly different characteristics of TIC and DVP between patients with benign and malignant masses. In 23 cases with benign masses, their DVP were significantly higher above the baseline than in those with malignant ones ( P = 0. 000), and in 15 cases with malignant lesions, their DVP were much shorter below the baseline than in those with benign ones, with statistical significance (P ＜0.05). The intensity of contrast medium, the time to reach its peak intensity and average transit time were all significantly higher in those with malignant masses than in those with benign ones (all P ＜ 0. 05 ). But, no statistical difference in the time of initial increasing between the two groups was found (P ＞ 0. 05). ConclusionsThere is significant difference in TIC and DVP of ultrasound imaging between benign and malignant masses, which if combined with contrast enhanced ultrasound parametric image processing can provide a more visualized quantitative information of benign and malignant ovarian masses with SonoLiver software.

The anti-HSV-2 effect of alumen: In vitro and in vivo experimental studies / 华中科技大学学报（医学）（英德文版）

This study investigated the anti-HSV-2 effect of alumen through in vitro and in vivo experiments. Viable cell counting was employed to assess the toxicity of alumen on Vero cells. The inhibition rate of HSV-2 was defined as the cytopathic effect (CPE) of the cells infected with the virus. Alumen suppositories of different concentrations were vaginally applied to the guinea pigs which were then infected with HSV-2 via a vaginal route. The clinical symptoms were observed and the local virus titer calculated. The results showed that alumen had an in vitro anti-HSV-2 effect by means of antiviral duplication, direct killing of the virus, and antiviral adsorption. Alumen suppositories of different concentrations could reduce or completely inhibit HSV-2 infection in guinea pigs. It was concluded that alumen had an in vitro anti-HSV-2 effect through multiple approaches and it could suppress in vivo vaginal HSV-2 infection of guinea pig to some extent.

Influencial Factors of Quality of Life for Post-stroke Patients / 中国康复理论与实践

@# ObjectiveTo explore the factors influencing quality of life (QOL) in post-stroke patients.MethodsThe data of 76 first-stroke patients in three general hospitals at 1 month and 4 months after stroke were selected with Stroke Impact Scale and analyzed with multiple linear regression.ResultsThe influencing factors of patients' QOL at one months after stroke were education background, type of care, gender, pathological regions, marriage and complications. The education background was the most important influencing factor. The influencing factors of 4 months after stroke were rehabilitation nursing, marriage, social support and gender. Rehabilitation nursing was the most important influencing factor.ConclusionThe many factors influence QOL for post-stroke patients and are changed with time.

Influencial Factors of Quality of Life for Post-stroke Patients / 中国康复理论与实践

@#ObjectiveTo explore the factors influencing quality of life (QOL) in post-stroke patients.MethodsThe data of 76 first-stroke patients in three general hospitals at 1 month and 4 months after stroke were selected with Stroke Impact Scale and analyzed with multiple linear regression.ResultsThe influencing factors of patients' QOL at one months after stroke were education background, type of care, gender, pathological regions, marriage and complications. The education background was the most important influencing factor. The influencing factors of 4 months after stroke were rehabilitation nursing, marriage, social support and gender. Rehabilitation nursing was the most important influencing factor.ConclusionThe many factors influence QOL for post-stroke patients and are changed with time.

A research about testing the stroke impact scale / 中国实用护理杂志

0.5.8 factors can explain the 80% of the whole variable. There were significant differences in the aspects of emotion,ADL/IADL,moving ability,participating and the total score(P