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Objective:To study whether curcumin inhibits the proliferation and promotes the apoptosis of nephroblastoma through activating the miR-192-5p/PI3K/Akt signaling pathway.Methods:CCK-8 assay was used to investigate the effects of curcumin on the proliferation of nephroblastoma SK-NEP-1 cells and the appropriate concentration. The apoptosis rate of SK-NEP-1 cells was detected by V-FITC/PI. Luciferase reporter assay was used to verify the binding activity between miR-192-5p and PI3K. RT-PCR was performed to detect the expression of miR-192-5p at mRNA level. Western blot was used to detect the expression of PI3K and Akt at protein level.Results:Curcumin could significantly inhibit the proliferation of SK-NEP-1 cells and induce cell apoptosis in a dose-dependent manner. RT-PCR results showed that curcumin could significantly increase the expression of miR-192-5p. In addition, miR-192-5p significantly inhibited cell proliferation, induced cell apoptosis, and enhanced the effects of curcumin on the proliferation and apoptosis of SK-NEP-1 cells. Luciferase reporter assay suggested that miR-192-5p could bind to PI3K. Western blot results showed that curcumin down-regulated the expression of PI3K and Akt at protein level by mediating the expression of miR-192-5p.Conclusions:Curcumin could inhibit the proliferation and induce the apoptosis of nephroblastoma cells through mediating the expression of miR-192-5p and further inhibiting the downstream PI3K/Akt signaling pathway.
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Objective To obsOrvO thO diffOrOnt manifOstations and diffOrOncOs of pOlvic laparoscopy in primary and sOcondary infOrtility patiOnts, and to clarify thO application of hystOroscopy combinOd with laparoscopy in thO diag-nosis and trOatmOnt of infOrtility.Methods A total of 120 infOrtilO womOn wOrO OnrollOd in Yiwu COntral Hospital from January 2015 to January 2017,of which 66 casOs wOrO primary infOrtility and 54 casOs wOrO sOcondary infOrtility. ThO infOrtility group was dividOd into 1 timO,2 timOs,3 timOs and abovO according to prOvious matOrnity. HystOroscopy combinOd with laparoscopy was usOd for pOlvic Oxploration in infOrtility patiOnts. ThO dOtOction of thO typO of disOasO, pOlvic adhOsions, tubal fluid and trOatmOnt wOrO analyzOd. Results (1) InfOrtilO patiOnts wOrO complicatOd with OndomOtriosis, pOlvic tubOrculosis, polycystic ovary, utOrinO fibroids, ovarian tumors, OndomOtrial polyps and othOr disOasOs.(2)ThOrO wOrO 94 casOs of pOlvic adhOsions(78.3% ),50 casOs of primary infOrtility(75.8% ),and 44 casOs in SOcondary infOrtility group(81.5% ).(3) Primary infOrtility adhOsion ratio of 75.8% ,sOcondary infOrtility adhO-sion ratio of 81.5% , thO proportion of adhOsions in matOrnal 1 timO patiOnts was 80.6% ,thO proportion of adhOsions in prOgnant womOn with 2 timOs was 83.3% ,thO proportion of adhOsions in prOgnant womOn with morO than thrOO timOs was 90.0% , and thO mOdian adhOsion ratO was 36.4% in thO sOcondary infOrtility casOs, thO proportion of sOvOrO adhOsion was 45.5%.(4) IntraopOrativO trOatmOnt of tubal fluid Oxamination conductOd in 240 strips.ThO probability of rOcanalization of thO primary infOrtility group was 17.78% ,and thO probability of rOcanalization was 18.75%.Conclusion (1) POlvic adhOsion is an important factor in causing infOrtility, primary infOrtility patiOnts also havO a grOatOr proportion of pOlvic, fallopian tubO disOasO.(2) ROpOatOd matOrnal history significantly incrOasOd pOlvic, fallopian tubO disOasO, which is an important factor causOd thO incrOasOd incidOncO of sOcondary infOrtility.
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BACKGROUND: Raman spectrum, compared with conventional detection technologies, is a rapid, non-invasive and label-free optical method. Its application has become an issue of concern in biomedical research. However, further studies are warranted to optimize the acquisition condition of Raman spectra from different stem cells. OBJECTIVE: To explore the effect of the wavelength of laser and the groove frequency of gratings to obtain the optimized parameter combination for Raman spectrum collection in human stem cells. METHODS: Using human mesenchymal stem cells as samples, the effects of different laser wavelengths (532, 38,785 nm) and different grating groove frequency (600, 1200, 1800 gr/mm) on Raman spectra were compared respectively. Then the different combinations of the wavelength and groove frequency were used and compared in terms of the spectra resolution and acquisition time, and the best acquisition condition was selected and applied in a comparison study on the Raman spectra from human mesenchymal stem cells and human embryonic stem cells. RESULTS AND CONCLUSION: The wavelengths of lasers and groove frequencies of gratings showed compound impacts on both the spikes at different wavenumbers and the ratio between spikes; the combination of 785 nm and 1200 gr/mm was confirmed to be the best spectrum features for human mesenchymal stem cells. The comparison of Raman spectra from human mesenchymal stem cells and human embryonic stem cells implies that the embryonic stem cells contain higher nucleic acids than the mesenchymal stem cells, while the mesenchymal stem cells appear to contain more proteins and lipids.
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After The Medical Institution Management Regulations was enacted by the State Council in 1994, 26 Chinese provinces and municipalities have enacted the local legislation of medical institution management.These local legislations make a full use of the space of legislation which the host law gives, reasonably determined the health administrative departments'management responsibilities at all levels, detailed the responsibility division for examina-tion and approval, and defined the medical institution practice behavior requirement.The local legislation has played a positive role for the rational medical resources allocation and standardized the local medical institutions practice or-der.But as time goes by and reform deeply, the weakness of these legislations has emerged.This is seen for the fact that they have not been repaired for many years, blindly repeat host law, heavily rely on the regulations with poor sta-bility , do not keep the pace with the system design times, violate the license law, and pay attention to the administra-tive examination and approval instead of the occupational behavior management.This paper makes full investigation on the existing relevant medical institution management laws, regulations and policies.Through the analysis of advan-tages and disadvantages of provincial legislations, some thinking on the medical institutions management is put for-ward.