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Aim To prepare the novel pyridostigmine bromide nanoemusion(PPNE)and study its release in vitro, and to investigate the intestinal absorption. Methods Pyridostigmine bromide (PB)and PPNE were tested by HPLC in pH 1 .2 HCl,pH 6.8,pH 7.4,pH 7.8 PBS.Rat single pass intestinal perfusion method was employed to investigate the absorption mechanism of PB and PPNE.Results PB release rate was faster than PB in the four release media;the intes-tinal absorption rate constant(Ka )and apparent perme-ability coefficient(Papp)of PPNE were increased in the duodenum,jejunum,ileum and colon segments.PB and PPNE had significant difference in the duodenum, jejunum,ileum and colon segments by t test (P <0.05).Conclusions PPNE can improve the bioavail-ability of drugs,increase the drugs permeability,sig-nificantly improve the absorption of the drugs in the in-testinal segments. PPNE has obviously sustained effects.
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Objective To observe the preliminary curative effect of transrectal ultrasound-guided lauromacrogol injection for treating benign prostate hyperplasia in elderly patients.Methods Forty-two patients with benign prostate hyperplasia combined with lower urinary tract symptonms received transrectal ultrasound-guided lauromacrogol injection.Then they were followed up after 3,6,12 months respectively. Prostate volume changes before and after treatment,international prostate symptom score(IPSS),postvoid residual volume (PRV ),maximum urinary flow rate (Qmax ) and features of contrast-enhanced ultrasonography before and after treatment were observed.Complications were analyzed.Results Before treatment,prostate volume,IPSS,PVR of patients were (57.3±1 7.7)ml,(28.3 ±5.2)points,(143.8±82.5) ml respectively,12 months after treatment,they reduced to (44.8 ±6.1 )ml,(8.8 ± 1 .4)points,(28.9 ±9.5 ) ml,there were statistical diffrences between before and after treatment(P <0.05 ).Qmax increased from (5.9±3.2)ml/s to (14.9 ±3.1 )ml/s (P <0.05).Contrast-enhanced ultrasonography showed filling defect sign in prostatic inner gland.No serve complications occurred.Conclusions Transrectal ultrasound-guided lauromacrogol injection for treating benign prostate hyperplasia has the characteristics of minimally invasive,safe,effective and economical.It applies to elderly patients who are unable or unwilling to have surgery.
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Objective To investigate specific anti-leukemia immune response induced by tumor lysates pulsed dendritic cells(TP-DC)in syngeneic bone marrow-transplanted mice.Methods From June 2002 to June 2003,the bone marrow cells were induced by using granulocyte-macrophage colony stimulating factor(mGM-CSF)and interleukin-4(mIL-4).DC was pulsed with L7212 tumor lysates at the immature stage.TP-DC was harvested on 7th day.615 mice immunized thrice with L7212 TP-DC starting at day 7 following BMT.Additional control groups were set up.The survival was observed.Results Immunization using TP-DC could induce L7212 cells-specific CTL responses that were statistically significant compared with control groups(F=391.77,P