RESUMO
Objective To explore the multi-resistance pattern of 88 strains of isoniazid-resistant Mycobacteria in recent years,so as to provide reference for prevention and treatment of tuberculosis.Methods Absolute concentration method was applied to performing drug susceptibility test for 181 isolates of Mycobacteria to 10 kinds of antitubercuIosis drugs. Results The primary resistance rate was 19.3%,and the acquired drug resistance rate Was 55.8%.The percentage of isoniazid-or double resistant-Mycobacteria was 37.6%.The Mycobacterium which was resistant to three or above drugs accounted for 85.2 %.Conclusion The majority of isoniazid-resistant Mycobacterium is a multi-resistant strain,which should be thought highly in prevention and treatment of tuberculosis to control or reduce the initial and acquired resistance rates.
RESUMO
Aim To establish experimental colitis model in Balb/c mice by Trinitrobenzene Sulfonic Acid (TNBS) enema. Methods Several doses of TNBS instilled into mice colon induced experimental colitis, then mortality rates of mice were observed. Severity of colitis was evaluated by the Disease Activity Index (DAI),Morphologic and Histologic analysis and Myeloperoxidase (MPO) analysis. We also observed the T cell proliferation of spleen. Results It showed that the mice mortality rate was increased when the mice were given the higher dose of TNBS. Most survived mice showed chronic inflammation in reduction colon. Histological examinations of the colon showed multiple erosive lesions and inflammatory cell infiltration composed of macrophages, lymphocytes, neutrophils in lamina propria and beyond mucosal layer. Some colon showed crypt distortion or reduction and high vascular density. Conclusion A TNBS dose of 1.5mg for each mouse was chosen for an appropriate experimental dose since the group showed less mortality rate and appropriate experimental colitis.