The Analysis of Auditory Brainstem Response Latency for Normal Hearing Newborn and Infants / 听力学及言语疾病杂志

Objective To investigate the characteristic changes of the auditory brainstem response (ABR) of normal hearing newborn and infants .Methods One hundred and forty -five cases (290 ears) normal hearing new-born and infants were enrolled for ABR test .Before that they passed the distortion product otoacoustic emissions and acoustic immitance test previously .According to age ,13 (26 ears) of 145 were included in the 1~7 days age group ,17 (34 ears) was in the 30~59 days age group ,20 (40 ears) in the 2~4 months age group ,19 (38 ears) in the 5~7 months age group ,17 (34 ears) in the 8~10 months age group ,18 (36 ears) in the 11~13 months age group ,12 (24 ears) in the 14~16 months age group ,13 (26 ears) in the 17~19 months age group ,16 (32 ears) in the more than 20~24 months age group .The control group was 20 (40 ears) normal hearing young persons (18~25 years old) .The variation of the peak latency and interpeak latency of waves were observed ,and compared with the normal young people .Results In 70 dB nHL click sound stimulus ,the peak latency and interpeak latency ofⅠ ,Ⅲ ,Ⅴ waves of normal hearing neonates and infants progressively shortened as they grew older .As they ap-proach 18 months or more old age ,their peak latency and interpeak latency of Ⅰ ,Ⅲ ,Ⅴ waves may be same as nor-mal young people .Conclusion With the growth of the age ,the peak latency and interpeak latency of Ⅰ ,Ⅲ ,Ⅴwaves of normal hearing newborn and infants gradually shortened .The reason may be related to the maturity of the auditory system .

Detection of TMPRSS2:ERG fusion gene in circulating prostate cancer cells / 亚洲男科学杂志(英文版)

<p><b>AIM</b>To investigate the existence of TMPRSS2:ERG fusion gene in circulating tumor cells (CTC) from prostate cancer patients and its potential in monitoring tumor metastasis.</p><p><b>METHODS</b>We analyzed the frequency of TMPRSS2:ERG and TMPRSS2:ETV1 transcripts in 27 prostate cancer biopsies from prostatectomies, and TMPRSS2:ERG transcripts in CTC isolated from 15 patients with advanced androgen independent disease using reverse transcription polymerase chain reaction (RT-PCR). Fluorescence in situ hybridization (FISH) was applied to analyze the genomic truncation of ERG, which is the result of TMPRSS2:ERG fusion in 10 of the 15 CTC samples.</p><p><b>RESULTS</b>TMPRSS2:ERG transcripts were found in 44% of our samples, but we did not detect expression of TMPRSS2:ETV1. Using FISH analysis we detected chromosomal rearrangements affecting the ERG gene in 6 of 10 CTC samples, including 1 case with associated TMPRSS2:ERG fusion at the primary site. However, TMPRSS2:ERG transcripts were not detected in any of the 15 CTC samples, including the 10 cases analyzed by FISH.</p><p><b>CONCLUSION</b>Although further study is required to address the association between TMPRSS2:ERG fusion and prostate cancer metastasis, detection of genomic truncation of the ERG gene by FISH analysis could be useful for monitoring the appearance of CTC and the potential for prostate cancer metastasis.</p>

Realization of surgical treatment in 248 patients with nodular goiter / 中国基层医药

Objective To study the surgical treatment of nodular goiter.Methods The surgical treatment effects of 248 cases with nodular goiter were reviewed.Results 123 paitients were cut off the merely nodle,83 patients suffered one side subtotal lobectomy,32 patients suffered doublr subtotal lobectomy, 10 patients suffered one side merely nodle plus oppside subtotal lobectomy, and 12 patients received operations twice,including 1 case (temporarily) of permanent injury of parathyroid, 1 case(temporarily) of injury of recurrent laryngeal never,3 cases of postoperative hemorrhage.All patients were followed up from 1 to 8 years,and no relapse was noted after operation.Conclusion One side thyroid gland nodle or nodles should cut off the merely nodle and it is unneceeary to deal with the blood vessel of the thyroid up and down,one side or double side thyroid gland nodles tightly should operated subtotal lobectomy, to ligate the blood vessel of the thyroid down, not to dispose the recuyyent laryngeal nerve,reserving the left over gland in cyst,and to prevent hypothyroidism and recurrent laryngeal nerve.

Determination of Platinum Group Elements by Neutron Activation Analysis and Inductively Coupled Plasma-Mass Spectroscopy Combined with Fire Assay Preconcentration / 分析化学

Two methods for the determination of platinum group elements were established based on neutron activation analysis (NAA) and inductively coupled plasma mass spectroscopy (ICP-MS) combined with fire assay preconcentration. Their analytical sensitivity,accuracy and applicability were discussed.The detection limits (ng/g)of NAA for Ru,Rh,Pd,Os,Ir and Pt are 0.5,0.5,0.3,0.1,0.01 and 0.2,respectively.whereas those of ICP-MS are 0.1 for Ru,0.05 for Rh,0.1 for Ir and 0.1 for Pt. Thus, both are complimentary for determination of platinum group elements. By the established methods the contents of platinum group elements in five geological reference materials were determined.