Effects of Celastrus orbiculatus Thunb.Extract on the Overexpression of mTOR in Human HepG2 Cells / 世界科学技术-中医药现代化

This study aimed at investigating the effects of the extract of Chinese herb,Nansheteng (C.orbiculatus Thunb.),on human HepG2 cells through the overexpression of mTOR.The GV238-mTOR recombinant plasmids were transfected into HepG2 cells using molecular biological technology.The expression level of mTOR was evaluated by means of relative activity of luciferase and western blot.Human hepatic carcinoma HepG2/mTOR++ cells were treated with C.orbiculatus extract in different concentrations (20,40,80,160 and 320 tg·mL-1) for 24 h.The mTOR protein expression was detected by western blot.It was found that the protein expression of mTOR in transfected HepG2 cells was significantly enhanced.C.orbiculatus extract significantly inhibited the proliferation of HepG2/mTOR++ cells.Simultaneously,C.orbiculatus extract inhibited mTOR at its protein level in a dose-dependent manner.In conclusion,we successfully constructed recombinant mTOR cloning vectors,and established the stable HepG2 cell line with the overexpression of mTOR.Besides,C.orbiculatus extract significantly inhibited mTOR protein expression in human hepatic carcinoma HepG2 cells.

Emphasis On the Cultivation of Six Kinds Of Consciousness for Medical Students / 中国医学伦理学

Medical students are the reserves of medical and health service in our country. Strengthening medical students' cultivation and improving their professional quality can play an important role in protecting people's health more efficiently. We must think highly of medical students' duty consciousness, creation consciousness, legal consciousness, problem consciousness, service consciousness and cooperation consciousness.We should enable medical students to have healthy medical professional personality and establish the foundation for the sustainable development of the health care industry of our country.