RESUMO
Protein kinase CK1 is a ser/thr protein kinase family which has been identified in the cytosol cell fraction, associated with membranes as well as in the nucleus. Several isoforms of this gene family have been described in various organisms: CK1 , CK1á, CK1δ, CK1å and CK1γ. Over the last decade, several members of this family have been involved in development processes related to wnt and sonic hedgehog signalling pathways. However, there is no detailed temporal information on the CK1 family in embryonic stages, even though orthologous genes have been described in several different vertebrate species. In this study, we describe for the first time the cloning and detailed expression pattern of five CK1 zebrafish genes. Sequence analysis revealed that zebrafish CK1 proteins are highly homologous to other vertebrate orthologues. Zebrafish CK1 genes are expressed throughout development in common and different territories. All the genes studied in development show maternal and zygotic expression with the exception of CK1å. This last gene presents only a zygotic component of expression. In early stages of development CK1 genes are ubiquitously expressed with the exception of CK1å. In later stages the five CK1 genes are expressed in the brain but not in the same way. This observation probably implicates the CK1 family genes in different and also in redundant functions. This is the first time that a detailed comparison of the expression of CK1 family genes is directly assessed in a vertebrate system throughout development.
Assuntos
Animais , Caseína Quinase I/genética , Regulação da Expressão Gênica no Desenvolvimento , Peixe-Zebra/embriologia , Sequência de Aminoácidos , Padronização Corporal , Clonagem Molecular , Caseína Quinase I/metabolismo , Primers do DNA , Perfilação da Expressão Gênica , Hibridização In Situ , Dados de Sequência Molecular , Filogenia , Reação em Cadeia da Polimerase Via Transcriptase Reversa , RNA Mensageiro , Alinhamento de Sequência , Peixe-Zebra/genéticaRESUMO
La somatostatina es una hormona con acción inhibitoria sobre otras hormonas, disminuye la motilidad, la secreción gastrointestinal y la circulación esplácnica, su vida media (T 1/2) es menor de tres minutos, por lo que su aplicación terpéutica es limitada; el análogo de ocho aminoácidos SMS 201 995 administrado por vía intravenosa tiene una T 1/2 de 43 minutos y una potencia 20 veces mayor que la hormona natural, por lo que se amplía su aplicación clínica, pero se desconoce la dosis más adecuada. Se hicieron 20 experimentos en 10 perros criollos, se comparó el efecto de 2 mcg/kg y 5 mcg/kg del octeótrico SMS 201 995, administrado por vía intravenosa en forma de bolo o en infusión continua. Para valorar la acción sobre la cirulación mesentérica se utilizó el equipo Vascular Minilab Portable Parcks 1059, con registro gráfico de doppler y fotopletismografía (PPG). La administración del SMS 201 995 produjo disminución de la PPG y en menor grado del doppler, la respuesta fue mayor con la dosis de 2 mcg/kg y con laa plicación en forma de bolo, aunque la diferencia no fue significativa. En dos animales hubo respuesta paradójica con aumento del flujo sanguíneo visceral. La administración del octeótrido SMS 201 995, sola o junto con otros procedimientos puede ser útil en el tratamiento de la hemorragia de vías digestivas