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1.
Asian Journal of Andrology ; (6): 83-88, 2003.
Artigo em Inglês | WPRIM | ID: wpr-300905

RESUMO

<p><b>AIM</b>To identify specifically expressed genes in the adult and fetal testes.</p><p><b>METHODS</b>A human testis cDNA microarray was established. Then the mRNA of adult and fetal testis was purified and probes were prepared by a reverse transcription reaction with the testis mRNA as template. The microarray was hybridized with probes of adult and fetal testes. The nucleic sequences of differentially expressed genes were determined and homologies were searched in the databases of the GenBank.</p><p><b>RESULTS</b>When hybridized with adult or fetal testis probes, the positive clones were 96.8 % and 95.4 %, respectively. Among these genes, one was a new testis-specific gene, which was named TSP1. TSP1 was highly expressed in human adult testis. The cDNA of TSP1 was 1,484 bp in length. The cDNA sequence of this clone was deposited in the Genbank (AF333098). TSP1 was also determined as Interim Gen Symbol (Unigene, No. Hs.98266). Protein analysis showed that TSP1 contained two functional domains: an N-terminal basic helix-loop-helix (bHLH) and a C-terminal leucine zipper (Zip). Homologous analysis showed that the 430 amino acid sequences deduced from the 1293 bp open reading frame (ORF) had a homology with the human gene FLJ2509 (AK098575). TSP1 had also a sequence homology with Spz 1 protein of mouse. Expression profiles showed that TSP1 was specifically and strongly expressed in the testis.</p><p><b>CONCLUSION</b>TSP1 is a gene highly expressed in adult testis. It may play an important role in spermatogenesis in the humans.</p>


Assuntos
Adulto , Humanos , Masculino , Pessoa de Meia-Idade , Sequência de Aminoácidos , Genética , Sequência de Bases , Genética , Fatores de Transcrição de Zíper de Leucina e Hélice-Alça-Hélix Básicos , Feto , Metabolismo , Expressão Gênica , Genes , Dados de Sequência Molecular , Análise de Sequência com Séries de Oligonucleotídeos , Homologia de Sequência de Aminoácidos , Testículo , Embriologia , Metabolismo , Fatores de Transcrição , Química , Genética , Metabolismo
2.
National Journal of Andrology ; (12): 247-249, 2002.
Artigo em Chinês | WPRIM | ID: wpr-322603

RESUMO

<p><b>OBJECTIVES</b>To investigate the relationship between chromosome balanced translocation t(1;12) (q24;q24) and spermatogenesis in infertile twin brothers.</p><p><b>METHODS</b>For twin brothers, karotype were determined. The levels of testosterone, FSH and LH were detected. YRRM1, DAZ and DYS240 were analyzed. In younger brother a biopsy was taken from testis.</p><p><b>RESULTS</b>Chromosome analysis for both twin brothers revealed a karotype of 46, XY, t(1;12) (q24;q24). Sperm count was less than 1.0 x 10(6)/ml. There was no deletion for YRRM1, DAZ and DYS240 gene on Y chromosome. Photomicrograph of seminiferous tubules showed the arrest of spermatogenesis.</p><p><b>CONCLUSIONS</b>Chromosome balanced translocation t(1;12) (q24;q24) may be the cause of the spermatogenesis arrest in infertile twin brothers. Gene in the point of translocation may be related to spermatogenesis.</p>


Assuntos
Adulto , Humanos , Masculino , Biópsia , Cromossomos Humanos Par 1 , Cromossomos Humanos Par 12 , Doenças em Gêmeos , Genética , Infertilidade Masculina , Genética , Espermatogênese , Genética , Testículo , Patologia , Translocação Genética , Genética
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