RESUMO
OBJECTIVE: To develop an UPLC method for simultaneous determination of seven components in Gardenia jasminoides, ie, gardoside, shanzhiside, deacetyl asperulosidic acid methyl ester, gardenoside (RG), genipin-1-β-D-gentiobioside, chlorogenic acid, and gardenoside to evaluate the quality of Gardenia jasminoides. METHODS: ACQUITY UPLC HSS T3 column was used for the UPLC analysis. The mobile phase was acetonitrile-0.05% phosphoric acid solution. Gradient elution was conducted at a flow rate of 0.2 mL·min-1. The column temperature was maitained at 30℃ and detection wavelength was set at 238 nm. A linear model was obtained through principal component analysis (PCA), and the scores of the principal components were used to evaluate the quality of Gardenia jasminoides Alba decoction pieces comprehensively. RESULTS: The seven components could be well separated from each other with good specificity, precision, repeatability, linearity, recovery rate and stability. The 25 Gardenia jasminoides Ellis samples and two Gardenia jasminoides Ellis var.grandiflora Nakai samples conformed to the quality requirements in the chapter of gardoside, shanzhiside, deacetyl asperulosidic acid methyl ester, gardenoside(RG), genipin-1-β-D-gentiobioside, chlorogenic acid, gardenoside. As the comprehensive evaluation shown, the quality of wild Gardenia jasminoides samples from Jiangxi province was better; Gardenia jasminoides from inland provinces excelled those from coastal provinces; and Gardenia jasminoides across Jiangxi province were of stable and higher quality. CONCLUSION: The method established in this study can effectively assay geniposide, gardoside, shanzhiside, deacetyl asperulosidic acid methyl ester, gardenoside and genipin gentiobioside in Gardenia jasminoides, thus it can be used for the quality control of Gardenia jasminoides.
RESUMO
In present study, an ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UHPLC-QTOF/MS)-based plant metabolomics approach was established to investigate the metabolic profiles of the leaves, main root, branch root, and rhizome of Mountain Cultivated Ginseng (MCG). The UHPLC-QTOF/MS data were subjected to principal component analysis (PCA) and orthogonal partial least squared discrimination analysis (OPLS-DA) to find the potential characteristic components of the four parts of MCG in a quick way. The four different parts could be separated into four different groups of phytochemicals according to the PCA scores. The chemical constituents in four parts of MCG were obviously different. The identities of 81 major peaks that were detected in the four parts of MCG and the potential markers were identified by comparison with the reference compounds or were tentatively assigned by matching the retention time,empirical molecular formula and fragment ions with those of the published compounds of the Panax species. This proposed analytical method is fast, accurate, and reliable for differentiating the different parts of MCG. Moreover, this study supplied a new method for the quality evaluation of other Chinese medicinal materials.