46XX pure gonadal dysgenesis with dysgerminoma along with leydig cells: a unique presentation

Authors report a unique case of 46XX gonadal dysgenesis, with dysgerminoma in one ovary and other streak ovary with hilar nests of leydig cells. It is exceptionally rare to find dysgerminoma in a dysgenetic gonad with no Y chromosome and so is the presence of leydig cells in the contralateral streak ovary in a patient with 46XX pure gonadal dysgenesis.

The perplexity of malignant ovarian mass in cystadenofibroma: a lesson learnt

The aim of this study is to report a rare case of ovarian cystadenofibroma with even scarcely observed mucinous type histopathological variant and to discuss the modalities which can contribute to prevent misdiagnosing it as a malignant tumor that may inappropriately lead to aggressive surgical interventions. A 40-year-old female presented with pain in lower abdomen and dysmenorrhea of 3 months duration and a large right adnexal mass which had all the features suggestive of malignancy in pre-operative investigations and even on gross overview and cut section. On the contrary histopathology reports confirmed its benign nature and reported it as mucinous cystadenofibroma. Role of Preoperative MRI and intraoperative frozen section cannot be overemphasized in distinguishing ovarian cystadenofibroma from malignant tumors, which can avoid an unnecessary extensive surgery.

Light wavelength dependent circadian and seasonal responses in blackheaded bunting.

Animals in the wild are exposed to daily variations in sun light, viz. duration, intensity and spectrum. Photosensitive blackheaded buntings (Emberiza melanocephala) were exposed to photoperiods differing in the length of light period, wavelengths and intensity. The effects of such light changes were measured on locomotor activity rhythm as well as seasonal responses like development of migratory restlessness: Zugunruhe, body mass and gonadal growth. The results show that the buntings are differentially responsive to light wavelengths and intensities and are indicative of a phase-dependent action of light on the circadian photoperiodic system. These birds seem to use changes in the light variables of the solar environment to regulate their circadian and seasonal responses.

Method Development and Validation of Levosalbutamol in Pure and Tablet Dosage Form by RP-HPLC.

A simple, rapid and accurate RP-HPLC method was developed for the determination of levosalbutamol in pure and tablet dosage form by RP-HPLC method using C18 BDS column (Phenomenex, 250 x 4.6 mm, 5 μm) in isocratic mode. The mobile phase consisted of Acetonitrile and buffer in the ratio of 20:80 (v/v) was used and maintain the pH 3. The flow rate was maintained at 1 mL/min and the injection volume was 20 μL . Detection wavelength with UV detector at 276 nm and run time was kept 10 min. The retention time of levosalbutamol was 5.4 min. The method was linear over the concentration range 7-12 μg/ml. The recovery was found to be 100.44± 0.27%. The validation of method was carried out utilizing ICH-guidelines. The described HPLC method was successfully employed for the analysis of pharmaceutical formulations.