Isolation of Pseudomonas aeruginosa from Bovine Mastitic Milk Sample Along with Antibiogram Study

A total of 4378 cattle and buffalo were screened during period of study (July 2018-June 19). Out of which 27 Pseudomonas aeruginosa were isolated. The isolates were confirmed phenotypically based on pigment production on nutrient agar. These were then confirmed by PCR amplification of species specific oligonucleotide sequences. All the 27 isolates amplified 956bp amplicon 16srRNA Pseudomonas aeruginosa species specific nucleotide sequence. The isolates were also checked for exo and aglD virulence associated genes. All of them amplified 540bp and 313bp amplicon of exo gene and aglD gene. All the isolates were subjected to antibiotic sensitivity testing. Most of the isolates showed highest sensitivity for levofloxacin, streptomycin and enrofloxacin followed by gentamicin, moxifloxacin and amikacin. Neomycin, cefoperazone and ceftriaxone were intermediate in action

Analysis of physicians’ strikes and their impact.

Resident doctors often resort to strikes for reasons concerning safety at the workplace, better working conditions, better remuneration, and policy issues such as caste-based reservations and appointments to institute positions . Although many articles have discussed whether or not physicians should resort to strikes, few have analysed the prevalence of strikes, their direct impact on healthcare delivery, and beneficial outcomes, if any, for physicians . We conducted a single centre retrospective study for analysing the same.