RESUMO
Medicine is a practical subject,which requires medical students to deepen and expand the understanding of the theory by clinical training,and establish clinical thinking.In our noviciate reform of obstetrics and gynecology,we have established the new problem-based teaching mode,which is an effective method in fostering the ability of information collection,innovation and practice,and have obtained more satisfactory results.
RESUMO
AIM To study antitumor activity of the extract denominated as UAP(Uric Antitumor Peptides isolated and purified from normal human urine). MOTHODS Proliferation inhibition assay was examined by cell counting and MTT method. Experiments in vivo were tested by treatment on tumor mice and acute toxicity on healthy mice. RESULTS UAP exerted strong dose dependent inhibition on the proliferation of three tumor cell lines, but not human normal leucocytes. Treatments on tumor mice show that UAP effectively inhibited tumor growth of mice HAC hepatoma, mice Lewis lung carcinoma and mice S 180 sarcoma with apparently dose dependent effect. Acute toxicity proved that UAP had very low acute toxicity in mice with LD 50 = 2 137 13 mg?kg -1 . CONCLUSION UAP has strong antitumor activity in vitro and in vivo. This provides a promising alternative antitumor treatment.