Interpretation of Detection and identification standard of hookworm—Hook-worm larvae coproculture techniques (WS/T 791—2021) / 中国血吸虫病防治杂志

Detection and identification standard of hookworm—Hookworm larvae coproculture techniques (WS/T 791—2021) is the first recommended technical standard for hookworm detection and species identification using the hookworm larvae coproculture technique in China. This standard was issued on November 23, 2021, and had been in effect since May 1, 2022. This article provides a detailed interpretation pertaining to the background, drafting process, main contents, and dos and don’ts for better understanding and application of this standard among professionals working in disease control and prevention institutions and medical institutions.

Epidemiological features of echinococcosis cases reported in the National Notifiable Disease Report System in Henan Province from 2010 to 2021 / 中国血吸虫病防治杂志

Objective To analyze the epidemiological characteristics of echinococcosis cases reported in the National Notifiable Disease Report System in Henan Province from 2010 to 2011, so as to provide insights into for echinococcosis control and surveillance. Methods The data pertaining to reported echinococcosis cases in Henan Province from 2010 to 2021 were retrieved from the National Notifiable Disease Report System, and a descriptive epidemiological analysis was performed using the software SPSS 22.0. Results A total of 150 echinococcosis cases were reported in Henan Province from 2010 to 2021, including 88 confirmed cases (58.67%) and 62 clinically diagnosed cases (41.33%), 77 cases reported by Henan Province (51.33%) and 73 cases reported by other provinces (48.67%). Echinococcosis cases were reported in each month, with 8 to 21 cases reported in each month, and the number of reported echinococcosis cases appeared no remarkable temporal changes. The echinococcosis cases were reported across 18 cities of Henan Province, with the highest number of cases reported in Zhoukou (17.33%) and Nanyang cities (17.33%) and the lowest number reported in Sanmenxia City (0.67%). The reported echinococcosis cases had a male to female ratio of 1.17:1, and the cases were found at each age group, with the the highest number of cases seen at ages of 20 to 59 years (73.33%). Farmer was the predominant occupation (63.33%), followed by housekeepers and the unemployed (12.67%). Of all reported echinococcosis cases, there were 25 local cases (16.67%) and 125 imported cases (83.33%), 144 cases reported by medical institutions (96.00%) and 6 cases reported by centers for disease control and prevention (4.00%). Conclusions Although imported echinococcosis cases were the predominant source of echinococcosis cases reported in the National Notifiable Disease Report System in Henan Province from 2010 to 2021, there were still sporadic local cases, and the emergence of local sources of infection cannot be excluded. Further expanded field surveys and surveillance of echinococcosis are required.

Progress of research on oncogene PTEN in thyroid malignant tumors / 中华内分泌外科杂志

The phosphatase and tensin homolog deleted on chromosome ten (PTEN) is a common oncogene located in the 10q23.3 region on the long arm of human chromosome l0, which regulates a variety of biological processes such as proliferation, survival, cell structure, motility, energy metabolism and genomic stability. Inactivation of PTEN is prevalent in almost all malignancies and correlates with tumor progression. Thyroid malignancies are among the most common endocrine malignancies, and PTEN has been shown to be critically associated with their development. The aim of this review is to describe the structural function of PTEN, as well as to summarize and discuss the recent findings of PTEN in thyroid malignancies.

Sequence analysis of Paragonimus internal transcribed spacer 2 and cyclooxygenase 1 genes in freshwater crabs in Henan Province / 中国血吸虫病防治杂志

Objective To investigate the sequences of internal transcribed spacer 2 (ITS2) and cyclooxygenase 1 (COX1) genes of Paragonimus metacercariae in freshwater crabs in Henan Province, identify the species of Paragonimus and evaluate its genetic relationships with Paragonimus isolates from other provinces in China. Methods Freshwater crabs were collected from 8 survey sites in Zhengzhou, Luoyang, Pingdingshan, Nanyang and Jiyuan cities of Henan Province from 2016 to 2021, and Paragonimus metacercariae were detected in freshwater crabs. Genomic DNA was extracted from Paragonimus metacercariae, and the ITS2 and COX1 genes were amplified using PCR assay, followed by sequencing of PCR amplification products. The gene sequences were spliced and aligned using the software DNASTAR, and aligned with the sequences of Paragonimus genes in the GenBank. Phylogenetic trees were created using the MEGA6 software with the Neighbor-Joining method based on ITS2 and COX1 gene sequences, with Fasciola hepatica as the outgroup. Results The detection rates of Paragonimus metacercariae were 6.83% (11/161), 50.82% (31/61), 18.52% (5/26), 8.76% (12/137), 14.29% (9/63), 17.76% (19/105), 18.50% (32/173) and 42.71% (41/96) in freshwater crabs from 8 survey sites in Zhengzhou, Luoyang, Pingdingshan, Nanyang and Jiyuan cities of Henan Province, with a mean detection rate of 19.46% (160/822), and a mean infection intensity of 0.57 metacercariae/g. The amplified ITS2 and COX1 gene fragments of Paragonimus were approximately 500 bp and 450 bp in lengths, respectively. The ITS2 gene sequences of Paragonimus metacercariae from 8 survey sites of Henan Province showed the highest homology (99.8% to 100.0%) with the gene sequence of P. skrjabini (GenBank accession number: MW960209.1), and phylogenetic analysis showed that the Paragonimus in this study was clustered into the same clade with P. skrjabini from Sichuan Province (GenBank accession number: AY618747.1), Guangxi Zhuang Autonomous Region (GenBank accession number: AY618729.1) and Hubei Province (GenBank accession number: AY618751.1), and P. miyazaki from Fujian Province (GenBank accession number: AY618741.1) and Japan (GenBank accession number: AB713405.1). The COX1 gene sequences of Paragonimus metacercariae from 8 survey sites of Henan Province showed the highest homology (90.0% to 100.0%) with the gene sequence of P. skrjabini (GenBank accession number: AY618798.1), and phylogenetic analysis showed that the Paragonimus in this study was clustered into the same clade with all P. skrjabini and clustered into the same sub-clade with P. skrjabini from Hubei Province (GenBank accession numbers: AY618782.1 and AY618764.1). Conclusions Paragonimus species from freshwater crabs in Henan Province were all characterized as P. skrjabini, and the ITS2 and COX1 gene sequences had the highest homology to those of P. skrjabini from Hubei Province. The results provide insights into study of Paragonimus in Henan Province and China.

Effects of highly active anti-retroviral therapy (HAART) on T lymphocyte activation and CD4 +CD45RA + T cell subsets in HIV/AIDS patients / 中华微生物学和免疫学杂志

Objective:To investigate the effects of highly active anti-retroviral therapy (HAART) on the activation of T lymphocytes and expression of CD4 + CD45RA + T cell subsets in HIV/AIDS patients. Methods:This study prospectively analyzed 105 HIV/AIDS patients undergoing HAART and 35 HIV-1-negative cases (healthy controls). Flow cytometry was used to detect the activation of T lymphocytes and the percentages of CD4 + CD45RA + T cell subsets in whole blood samples taken from healthy controls and HIV/AIDS patients before and after therapy. Results:The activation of T lymphocytes was significantly enhanced in the 105 HIV/AIDS patients than in the healthy controls before treatment ( P<0.01). The activated T lymphocytes gradually decreased after HAART. Firstly, CD4 + CD38 + HLA-DR + , CD8 + CD38 + and CD8 + HLA-DR + T lymphocytes decreased one month after therapy ( P<0.05). Then, four indicators of T lymphocyte activation including the expression of CD8 + CD38 + HLA-DR + T lymphocytes decreased significantly six months after therapy ( P<0.01). The percentage of CD8 + CD38 + HLA-DR + T lymphocytes detected 12 months after therapy was significantly lower than that analyzed six months after therapy ( P<0.01). No significant difference was found in the expression of the other three indicators for activation ( P>0.05). Twelve months after therapy, the four indicators for T lymphocyte activation in HIV/AIDS patients were still significantly higher than those of the control group ( P<0.01). The percentages of CD4 + CD45RA + T lymphocytes in HIV/AIDS patients were significantly lower than those in healthy controls before and 12 months after treatment ( P>0.05). Conclusions:HAART could reduce immune activation after six months of treatment, but could not reverse the activation nor restore the expression of CD4 + CD45RA + T lymphocytes in HIV/AIDS patients.

Investigate thenovel coronaviruses in external environmentof COVID-19 / 中华实验和临床病毒学杂志

Objective To understand the distribution of novel coronaviruses in the external environment of confirmed COVID-19 cases. Methods Environmental surface swab specimens such as bed rails, doorknob, closestool, hand washing sink, table, locker,ward pager, mobile phone, cup, clothes, were collected from the sentinel hospital of COVID-19, and samples were collected for the nucleic acid detection by RT-PCR. Results A total of 150 environmental samples were collected from 30 confirmed COVID-19 cases, 6 samples were determined to be novel coronaviruses postive (positive rate 4.00%). The total 14 mobile phone showed 3 novel coronaviruses positive.Among the 30 confirmed COVID-19 cases, 6 cases (positive rate 20.00%)were found novel coronaviruses in the external environment. Conclusions Novel coronaviruses exists in external environment of confirmed COVID-19 cases, which indicates the potential risk of COVID-19 infection.

Stratified sampling survey of major human parasitic diseases in Henan province / 中华流行病学杂志

Objective To understand the prevalence of major human parasitic diseases and related factors in Henan province.Methods This stratified sampling survey was carried out according to the requirement of national survey protocol of major human parasitic diseases,2014-2015.The prevalence of soil-transmitted helminths infection,taeniasis and intestinal protozoiasis were surveyed in 104 sites selected from 35 counties (districts) and the prevalence of clonorchiasis was surveyed in 62 sites selected from 37 townships.In each survey spot,250 persons were surveyed.A total of 26 866 persons and 15 893 persons were surveyed.Modified Kato-Katz thick smear was used to detect the eggs of intestinal helminthes.Tube fecal culture was used to identify the species of hookworm.The Enterobius eggs were detected in children aged 3 to 6 years by using adhesive tape.The cyst and trophozoite of intestinal protozoa were examined with physiological saline direct smear method and iodine stain method.Results The overall infestation rate of intestinal parasites was 2.02％ in Henan,and the worm infection rate was higher than protozoa infection rate.Fourteen kinds of intestinal parasites were found,including nematode (5 species),trematode (2 species),and protozoan (7 species).The infection rate of Enterobius vermicularis was highest,and Qinba Mountain ecological area had the highest infestation rate of intestinal parasites in 4 ecological areas of Henan.There was no significant difference in intestinal parasite infection rote between males and females (x2=3.630,P=0.057),and the differences in intestinal parasite infection rate among different age groups had significance (x2=124.783,P=0.000 1).The infection rate reached the peak in age group ≤9 years and the major parasite was Enterobius vermicularis.Furthermore the overall human infection rate of parasite showed a downward trend with the increase of educational level of the people (x2=70.969,P=0.000 1),the differences had significance (x2=120.118,P=0.000 1).For different populations,the infection rate of intestinal parasites was highest among preschool children.The infection of intestinal helminth was mainly mild,only 2 severe cases were detected.The infection rate of Clonorchis sinensis in urban residents was only 0.006％.Logistic regression analysis showed that being preschool children (x2=15.765,P=0.000 1) and drinking well water (X2=45.589,P=0.000 1) were the risk factors for intestinal parasite infection,and annual income per capita of farmers was the protective factor against intestinal parasite infection.The infection rates of protozoa and intestinal parasites decreased sharply compared with the results of previous two surveys,and the rate of intestinal helminth infection also dropped sharply compared with the second survey.The numbers of protozoa,helminth and intestinal parasites detected in this survey were all less than the numbers found in the previous two surveys.Conclusions Compared the results of three surveys in Henan,the infection rate of protozoa and intestinal parasites showed a downward trend.The prevention and treatment of Enterobius vermicularis infection in children should be the key point of parasitic disease control in the future.

Stratified sampling survey of major human parasitic diseases in Henan province / 中华流行病学杂志

Objective To understand the prevalence of major human parasitic diseases and related factors in Henan province.Methods This stratified sampling survey was carried out according to the requirement of national survey protocol of major human parasitic diseases,2014-2015.The prevalence of soil-transmitted helminths infection,taeniasis and intestinal protozoiasis were surveyed in 104 sites selected from 35 counties (districts) and the prevalence of clonorchiasis was surveyed in 62 sites selected from 37 townships.In each survey spot,250 persons were surveyed.A total of 26 866 persons and 15 893 persons were surveyed.Modified Kato-Katz thick smear was used to detect the eggs of intestinal helminthes.Tube fecal culture was used to identify the species of hookworm.The Enterobius eggs were detected in children aged 3 to 6 years by using adhesive tape.The cyst and trophozoite of intestinal protozoa were examined with physiological saline direct smear method and iodine stain method.Results The overall infestation rate of intestinal parasites was 2.02％ in Henan,and the worm infection rate was higher than protozoa infection rate.Fourteen kinds of intestinal parasites were found,including nematode (5 species),trematode (2 species),and protozoan (7 species).The infection rate of Enterobius vermicularis was highest,and Qinba Mountain ecological area had the highest infestation rate of intestinal parasites in 4 ecological areas of Henan.There was no significant difference in intestinal parasite infection rote between males and females (x2=3.630,P=0.057),and the differences in intestinal parasite infection rate among different age groups had significance (x2=124.783,P=0.000 1).The infection rate reached the peak in age group ≤9 years and the major parasite was Enterobius vermicularis.Furthermore the overall human infection rate of parasite showed a downward trend with the increase of educational level of the people (x2=70.969,P=0.000 1),the differences had significance (x2=120.118,P=0.000 1).For different populations,the infection rate of intestinal parasites was highest among preschool children.The infection of intestinal helminth was mainly mild,only 2 severe cases were detected.The infection rate of Clonorchis sinensis in urban residents was only 0.006％.Logistic regression analysis showed that being preschool children (x2=15.765,P=0.000 1) and drinking well water (X2=45.589,P=0.000 1) were the risk factors for intestinal parasite infection,and annual income per capita of farmers was the protective factor against intestinal parasite infection.The infection rates of protozoa and intestinal parasites decreased sharply compared with the results of previous two surveys,and the rate of intestinal helminth infection also dropped sharply compared with the second survey.The numbers of protozoa,helminth and intestinal parasites detected in this survey were all less than the numbers found in the previous two surveys.Conclusions Compared the results of three surveys in Henan,the infection rate of protozoa and intestinal parasites showed a downward trend.The prevention and treatment of Enterobius vermicularis infection in children should be the key point of parasitic disease control in the future.

Changes in CD4+ T lymphocyte subset distribution and homeostasis in MSM population with differ-ent stages of HIV infection / 中华微生物学和免疫学杂志

Objective To investigate the changes in the percentages of CD4+T lymphocyte subsets and the homeostasis of T lymphocytes among MSM ( men who have sex with men) population with different stages of HIV-1 infection. Methods A total of 166 untreated MSM with HIV infection were enrolled and di-vided into three groups including early HIV infection (EHI, n=38) , HIV (n=94) and AIDS (n=34) groups. Sixty-two MSM negative for anti-HIV antibody were selected as healthy controls. Blood samples were collected into EDTA tubes and detected to analyze the changes in the distribution of CD4+ T cells and CD8+T lymphocyte subsets ( CD4+ CD45RA+, CD8+ CD28+, CD4+ CD25+ CD127-) and the percentages of activated (CD38, HLA-DR) and apoptotic cells (CD95) with disease progression by flow cytometry. Re-sults The expression of CD4+CD45RA+ T lymphocytes gradually decreased with the progression of AIDS. The percentage of CD4+CD45RA+ T lymphocytes in HIV group was lower than that of the control group, but higher than that of the AIDS group (P=0. 015, P=0. 000). No significant difference was found between the EHI and the control groups (P>0. 05). CD8+CD28+T cells were significantly reduced in the EHI group and remained at a low level with disease progression. No significant difference in the proportion of CD4+CD25+CD127- T cells was observed among all groups (P>0. 05). The percentage of CD4+CD38+HLA-DR+T cells increased gradually and the highest level was detected in the AIDS group, followed by those in the HIV, EHI and control groups (P<0. 01). The percentages of CD8+CD38+, CD8+HLA-DR+, CD8+ CD38+HLA-DR+and CD8+CD95+T cells in the EHI, HIV and AIDS groups were significantly higher than those in the control group (P<0. 01), but there was no significant difference among the former three groups (P>0. 05). Con-clusion HIV infection caused the changes in the numbers and functions of T lymphocyte subsets and accel-erated the activation and apoptosis of T lymphocytes, which aggravated the T lymphocyte immune dysfunction even further. A comprehensive analysis of the alterations in different T cell subsets would be conducive to re-flect the immune deficiency and the severity of disease. CD4+ and CD8+ T cells were activated in the early stage of HIV infection, which indicated that studying the immune response during that stage might help to understand their roles in disease progression.

The effect of drug transporter gene polymorphisms on susceptibility to imatinib in chronic myelogenous leukemia / 中国肿瘤临床

As a first generation tyrosine kinase inhibitor, imatinib is the gold standard drug for the clinical treatment of chronic myelog-enous leukemia. However, interindividual differences in resistance and response to imatinib have been widely observed. In vivo and in vitro studies have confirmed that ATP-binding cassette transporters, organic cation transporters, and organic anion transporting poly-peptides have a large effect on imatinib pharmacokinetics and pharmacodynamics. In addition, the gene polymorphisms of drug trans-porters can directly or indirectly influence the intracellular concentration of imatinib, resulting in differences in treatment efficacy among chronic myelogenous leukemia patients. This review profiles the effect of drug transporter gene polymorphisms on susceptibili-ty to imatinib in chronic myelogenous leukemia so as to provide reference to further clinical researches.

Exploration and application of polyhydroxyalkanoates and polylactic acid membranes during glaucoma filtering surgery and interlaminar transplantation in rabbits / 中国比较医学杂志

Objective To evaluate the effect of two polymer membranes, polyhydroxyalkanoates(PHA)and polylactic acid(PLA)during glaucoma filtration surgery(GFS),and to evaluate the morphology of membranous PHA after interlamellar implantation. Methods Twenty-eight New Zealand white rabbits were chosen and twenty-four of them were randomly divided into 6 groups(n=4):the PHA-low group,PHA-high group,PLA-low group,PLA-high group,positive control group(MMC group)and blank control group. The rabbits in each group received GFS. The corresponding polymer membranes were implanted under the scleral flap,while the MMC group was treated with 0.2 mg/mL mitomycin C(MMC) for 3 minutes,and the blank control group was treated without extra drugs. The intraocular pressure(IOP)was examined at 0 d,1 d, 3 d, 7 d, 14 d, 28 d and 84 d after GFS. The corneal layers of four rabbits were implanted with PHA membranes and the corneal morphological changes were observed after 84 d. Results The IOP of the PHA-low and PLA-high groups was lower than that of the blank control group at 84 d after GFS(P < 0.05),and was similar with that of the MMC group(P> 0.05). Morphological studies showed that there were no collagenous fibers filling in the duct, and the collagenous fibers around the membranes were generally arranged in parallel. There were no obvious changes in the peripheral collagen structure after implantation of PHA membranes between the corneal layers. Conclusions Application of PHA and PLA membranes during GFS in rabbits may maintain the level of IOP,and the effect is similar with MMC. The mechanism may be achieved through the mechanical blocking of fibrous tissue.

Evaluation of endometriosis fertility index in follow-up treatment of endometriosis combined with infertility patients after laparoscopic surgery / 中华妇产科杂志

Objective To explore the application of endometriosis fertility index (EFI) in guidance after laparoscopic surgery of endometriosis patients combined with infertility and to explore methods to improve pregnancy rate in different EFI groups. Methods A prospective research was done in endometriosis patients combined with infertility in Beijing Obstetrics and Gynecology Hospital from January 2010 to June 2011, after laparoscopic surgery, these 146 patients were divided into 3 groups by EFI score. Using different pregnancy guidance, these patients had 5 years follow-up. Results (1) The 5 years overall pregnancy rate was 89.0%(130/146). The pregnancy rate was 95.7%(45/47) in EFI≥9 group, 92.8%(77/83) in EFI 5-8 group and 8/16 in EFI≤4 group, three groups were all reach satisfactory pregnancy rate;the rate of the first two groups had no statistically significance (P=0.498), but had significant difference with the last group (P<0.01). (2) In EFI≥5 patients, pregnancy rate was the highest in 6 months after operation;in EFI≥9 group, the pregnancy rate was 66.7%(30/45), and EFI 5-8 group was 50.6%(39/77). (3) EFI≥9 group had the highest natural pregnancy rate [83.6% (46/55)], natural pregnancy rate was significant statistical different in different EFI groups (P=0.001). Conclusions EFI score is a useful evaluation in predicting and guiding pregnancy in endometriosis patients combined with infertility after laparoscopic surgery. EFI score guidance, strict post-operation management and positive pregnancy scheme could significantly improve the pregnancy rate of endometriosis patients with infertility.

The study on Ezscan screening for dysglycaemia in Chongqing area / 重庆医学

Objective To assess the screening capacity of Ezscan for abnormal glucose metabolism .Methods A total of 1 732 permanent residents of Chongqing city underwent physical examination from August 2013 to August 2014 were selected as subjects ,questionnair survey ,physical examination and Ezscan measurement were performed .Additionally ,the levels of fasting blood‐glucose(FPG) ,OGTT 2h blood glucose ,HbA1c and serum lipid were detected .Results The prevalence rate of abnormal glucose metabolism for residents with high Ezscan score was higher than that in those with low Ezscan score .The AUC of Ezscan screening for abnormal glucose metabolism was 0 .616 ,the optimum diagnosis tangent point was 24 .0% ,the sensitivity was 67 .0% .The optimum diagnosis tangent point of FPG was 5 .5 mmol/L for abnormal glucose metabolism ,the sensitivity and speci‐ficity was 70 .5% and 61 .7% respectively ,and the AUC was 0 .824 .The sensitivity of FPG for screening abnormal glucose metabo‐lism was 47 .2% ,when the cut‐off value of FPG was 6 .1 mmol/L .Conclusion The sensitivity of Ezscan for screening abnormal glucose metabolism is higher than that of FPG (when the cut‐off value was 6 .1 mmol/L) ,Ezscan can be used as an efficiency meth‐od for early detection of abnormal glucose metabolism in the asymptomatic population .

Relationship between the prevalence rate of hypertension and sleeping time in Chongqing area / 重庆医学

Objective To investigate the prevalence rate of hypertension ,overweight ,obesity and obesity/overweight ,thus to explore the relationship between hypertension and sleeping time .Methods Healthy individuals having physical examinations in Health Management Center of Southwest Hospital were randomly selected to take questionnaire and physical examination ,and then we analyzed the correction relationship of hypertension and sleeping time .Results The prevalence of overweigh ,obesity ,hyperten‐sion and perhypertension was 36 .68% ,11 .36% ,22 .14% and 33 .43% respectively .The prevalence rate of hypertension for men was 2 fold as that of women .There was a negative correlation between hypertension and sleeping time .The prevalence of stage 2 hypertension tended to U.People who sleep time less than 5 h have more risk for Isolated systolic hypertension than who sleeping 6 h .Conclusion There is a high risk of hypertension and stage 2 and stage 3 hypertention for people whose sleep time less than 5 h or more than 10 h .

The associations between idiosyncratic adverse drug reactions and HLA alleles and their underlying mechanism / 药学学报

With the advent of Twenty-First century, more and more genome-wide association studies (GWAS) showed that idiosyncratic adverse drug reactions (ADRs) were closely related with human leukocyte antigen (HLA) alleles, such as the associations of abacavir-HLA-B*5701, allopurinol-HLA-B*5801, and carbamazepine-HLA-B*1502, etc. To explore the mechanisms of these idiosyncratic drug reactions, hapten hypothesis, danger signal hypothesis, pharmacological interaction (P-I) concept and autoimmune mechanism are proposed. In this paper, recent GWAS studies on the HLA-mediated adverse drug reactions and underlying mechanism are reviewed in detail.

Determination of Dehydroisoandrosterone Sulfate in Human Plasma by LC-MS / 中国药房

OBJECTIVE:To determine the concentration of dehydroisoandrosterone sulfate (DHEAS) in human plasma by LC-MS.METHODS:With estrogen sulfate (ES) served as an internal standard,the plasma samples were deproteinized with acetonitrile,extracted by solid phase,hydrolyzed and derivatized.Then the concentration of DHEAS was determined by HPLC-MSD on Agilent SB C18 with column temperature kept at 40℃.The mobile phase consisted of acetonitrile-water (in gradient elution).Atmosphere pressure chemical ion source in negative ion detection model was employed.The ions selected for SIM (selected ion monitoring) quantitative analysis included m/z 490.0 (DHEAS ) and m/z 472.1(ES)[M-H]-.RESULTS:The linear range of DHEAS was 250.0～320.0 ng?mL-1(r=0.999 4).The extraction recovery of the simulated human albumin samples ranged from 71.1%～78.9% and its relative recovery ranged from 98.3%～101.4%.Both the intra-day RSD and inter-day RSD were less than 10%.The mean concentration of DHEAS in 15 health aged male volunteers was (981.6?353.4) ng?mL-1.CONCLUSION:The method is simple,practical,accurate and sensitive,and it is applicable for the determination of plasma concentration of DHEAS.

Study on the mRNA expression of potassium channel gene Kv1-5 in the developing rat brain / 中国药理学通报

AIM To investigate the patterns of potassium channel gene Kv 1 5 mRNA expression in the developing rat brain by using RNase protection assay. METHODS (1) RNA was extracted from brain tissues by one step method, (2) The preparation of DNA template, (3) Synthesis of RNA probe in vitro transcription, (4) RNA:RNA. RESULTS Kv 1 5 was absent in embryo tissue, but significantly increased at postnatal day 25 with adult rat, the mRNA expression did not show any change. CONCLUSION there is close relationship between Kv 1 5 mRNA expression is pertinent to the development and function differentiation of rat brain.