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1.
Chinese Journal of Contemporary Pediatrics ; (12): 401-407, 2023.
Artigo em Chinês | WPRIM | ID: wpr-981970

RESUMO

A boy, aged 16 months, attended the hospital due to head and facial erythema for 15 months and vulva erythema for 10 months with aggravation for 5 days. The boy developed perioral and periocular erythema in the neonatal period and had erythema and papules with desquamation and erosion in the neck, armpit, and trigone of vulva in infancy. Blood gas analysis showed metabolic acidosis; the analysis of amino acid and acylcarnitine profiles for inherited metabolic diseases and the analysis of organic acid in urine suggested multiple carboxylase deficiency; genetic testing showed a homozygous mutation of c.1522C>T(p.R508W) in the HLCS gene. Finally the boy was diagnosed with holocarboxylase synthetase deficiency and achieved a good clinical outcome after oral biotin treatment. This article analyzes the clinical data of a child with holocarboxylase synthetase deficiency and summarizes the etiology, diagnosis, and treatment of this child, so as to provide ideas for clinicians to diagnose this rare disease.


Assuntos
Humanos , Masculino , Lactente , Biotina/uso terapêutico , Deficiência de Holocarboxilase Sintetase/tratamento farmacológico , Homozigoto , Mutação , Doenças Raras/tratamento farmacológico
2.
Chinese Journal of Contemporary Pediatrics ; (12): 98-102, 2021.
Artigo em Chinês | WPRIM | ID: wpr-879816

RESUMO

A girl, aged 22 months, attended the hospital due to recurrent vulvar rashes for more than half a year. Skin biopsy showed Langerhans cell histiocytosis, and evaluation of systemic conditions showed no systemic involvement. Therefore, the girl was diagnosed with Langerhans cell histiocytosis (skin type). In conclusion, for rashes on the vulva alone, if there are no specific clinical manifestations, the possibility of Langerhans cell histiocytosis should be considered after molluscum contagiosum, sexually transmitted diseases, and Fordyce disease are excluded.


Assuntos
Feminino , Humanos , Lactente , Deficiências do Desenvolvimento , Exantema/etiologia , Histiocitose de Células de Langerhans , Doenças da Vulva/diagnóstico
3.
Journal of Zhejiang University. Medical sciences ; (6): 17-23, 2010.
Artigo em Chinês | WPRIM | ID: wpr-259247

RESUMO

<p><b>OBJECTIVE</b>To investigate the effect of tBHQ and sulforaphane on the protein expression in Nrf2-ARE signaling pathway of Caco2 cells.</p><p><b>METHODS</b>Human colorectal carcinoma Caco2 cells were treated with 20 micromol/L tBHQ and 5 micromol/L sulforaphane (SFN) respectively. Real time PCR, Western blotting and immunoflourescence staining (IF) were performed to measure the target gene expression.</p><p><b>RESULTS</b>Nrf2, AKR1C1 and NQO1 protein expressions were increased time-dependently in Caco2 cells after treatment with tBHQ and SFN. Time-course experiments showed that tBHQ and SFN increased the accumulation of Nrf2, and concomitantly increased the protein levels of AKR1C1 and NQO1. Real-time PCR and Western blotting showed that tBHQ and SFN significantly increased the expression of Nrf2 at 8h after the treatment, and AKR1C1 and NQO1 at 16 h. Confocal microscopy technique showed that Nrf2 accumulated in the nucleus at 6-8 h after treatment with tBHQ. After 1 h treatment with tBHQ the nuclear Nrf2 maintained at elevated level for at least 4 h with tBHQ withdrawn.</p><p><b>CONCLUSION</b>tBHQ and SFN induced nuclear accumulation of Nrf2 and activated Nrf2-dependent regulation of ARE-mediated gene expression in Caco2 cells. In addition, the results provide experimental evidence for choosing the dose and frequency of the inducer in cancer chemoprevention study and in developing inhibitors of Nrf2-ARE signaling pathway.</p>


Assuntos
Humanos , Anticarcinógenos , Farmacologia , Antioxidantes , Metabolismo , Farmacologia , Células CACO-2 , ATPases Transportadoras de Cálcio , Hidroquinonas , Farmacologia , Isotiocianatos , Fator 2 Relacionado a NF-E2 , Genética , Metabolismo , Fisiologia , Estresse Oxidativo , Genética , Fisiologia , Elementos de Resposta , Fisiologia , Transdução de Sinais , Tiocianatos , Farmacologia
4.
Journal of Zhejiang University. Medical sciences ; (6): 24-29, 2010.
Artigo em Chinês | WPRIM | ID: wpr-259246

RESUMO

<p><b>OBJECTIVE</b>To investigate the effects of transcriptional inhibitors 5, 6-dichloro-1-b-D-ribofuranosylbenzimidazole (DRB) and alpha-Amanitin on the localization of Nrf2 in the nucleus.</p><p><b>METHODS</b>A549 cells were treated with DRB (50 mg/L) or alpha-Amanitin (2.5 mg/L)for 1 h and 6 h in serum-free medium, respectively. The expressions of Nrf2, HO-1, NQO1 and AKR1C were detected by Western blotting analysis. The localization of Nrf2 was determined by laser scanning confocal microscopy after cells were treated with either DRB or agr:-Amanitin for 1 h.</p><p><b>RESULTS</b>The expressions of Nrf2 and Nrf2-ARE gene batteries HO-1, AKR1C and NQO1 were decreased after 6 h treated with either DRB or alpha-Amanitin. The expression of SC35 was up-regulated but RNA Pol II was down-regulated; Y12 and NPC did not significantly change. The localization of Nrf2 in the cell nucleus did not change significantly.</p><p><b>CONCLUSION</b>DRB and alpha-Amanitin can down-regulate the expression of Nrf2 and its targeting proteins HO-1, AKR1C and NQO1, but may have no effect on the localization of Nrf2.</p>


Assuntos
Humanos , 20-Hidroxiesteroide Desidrogenases , Genética , Metabolismo , Alfa-Amanitina , Farmacologia , Carcinoma Pulmonar de Células não Pequenas , Genética , Metabolismo , Patologia , Linhagem Celular Tumoral , Diclororribofuranosilbenzimidazol , Farmacologia , Heme Oxigenase-1 , Genética , Metabolismo , Neoplasias Pulmonares , Metabolismo , Patologia , Fator 2 Relacionado a NF-E2 , Genética , Metabolismo , Inibidores da Síntese de Ácido Nucleico , Farmacologia
5.
Journal of Zhejiang University. Medical sciences ; (6): 30-36, 2010.
Artigo em Chinês | WPRIM | ID: wpr-259245

RESUMO

<p><b>OBJECTIVE</b>To investigate the effect of Luteolin alone or combination with chemotherapentic drugs on the cytoxicity of cancer cells.</p><p><b>METHODS</b>Cultured A549, Hela, MCF-7, AGS, MGC-803, Caco2 and HepG2 cells were treated with Luteolin or the combination of Luteolin with other chemotherapeutic agents (Bexarotene, Cisplatin and Bleomycin). Cell viability was measured by MTS assay and IC(50) was calculated.</p><p><b>RESULTS</b>The IC(50) of Bexarotene to Hela cells was 2 micromol/L, but with the combination of 5 micromol/L of Luteolin that reduced to 0.2 micromol/L. However, the combination of Bexarotene and Luteolin did not show significant benefit in MGC-803, HepG2 cells, Caco2 and MCF-7 cells. The IC(50) of Cisplatin to Hela cells was over 30 micromol/L,but it decreased to 3 micromol/L in the presence of 5 micromol/L Luteolin; Luteolin also sensitized Cisplatin in MGC-803, HepG2 and A549 cells studied. The IC(50) of Bleomycin to Hela cells was over 100 micromol/L, but it was about 1 micromol/L in the presence of 5 micromol/L Luteolin. A549 cells were resistant to Bleomycin with an IC(50) of 100 micromol/L, 10 micromol/L Luteolin greatly enhanced the cytotoxicity of Bleomycin to the cells with the IC(50) of about 10 micromol/L. The inhibitions of MGC-803, HepG2, A549 and AGS cells didn't change by combination of Luteolin.</p><p><b>CONCLUSION</b>Low concentration of Luteolin has little toxic effect on the cancer cell lines tested in the study, but it can sensitize chemotherapeutic drugs in various cancer cell lines.</p>


Assuntos
Humanos , Antineoplásicos , Farmacologia , Neoplasias da Mama , Patologia , Linhagem Celular Tumoral , Proliferação de Células , Sinergismo Farmacológico , Neoplasias Pulmonares , Patologia , Luteolina , Farmacologia , Neoplasias , Patologia
6.
Journal of Zhejiang University. Medical sciences ; (6): 501-506, 2006.
Artigo em Chinês | WPRIM | ID: wpr-332116

RESUMO

<p><b>OBJECTIVE</b>To investigate the effect of cytomegalovirus (CMV) infection on actin and microfilament in human embryo fibroblast cells (HF) and its relationship with CMV replication.</p><p><b>METHODS</b>Cell morphology was observed after the infection of CMV. Western-blot was used to measure the expression levels of beta-actin, G-actin and F-actin proteins. CMV immediately early antigen (CMV IE) in HF cells was analyzed by indirect immunofluorescence assay. Microfilament alteration was determined by cytoskeleton fluorescence probe.</p><p><b>RESULT</b>CMV IE was demonstrated in more than 95% of HF cells after infection, which was primarily located in nucleus. The shape of HF cells changed from thin shuttle like to round and thick ball like, even escaping from wall after infection by CMV. Compared with control group, the expression of G-actin protein increased at 24 h of CMV infection (0.941 +/-0.061 compared with 0.714 +/-0.119, P <0.05), then decreased at 72 h, 96 h respectively(0.218 +/-.035, 0.230 +/-0.055 compared with 0.714 +/-0.119, P <0.05). The levels of F-actin in infected cells gradually decreased at 24 h, 72 h and 96 h compared with control HF cells (0.256 +/-0.021, 0.127 +/-0.032, 0.026 +/-0.008 compared with 0.373 +/-0.050, P<0.05). In infected HF cells, microfilaments were found ruptured, arranged turbulently. Cells fused and fluorescence density of microfilament markedly reduced.</p><p><b>CONCLUSION</b>Cytomegalovirus can induce alteration of actins and microfilament, which may be associated with its infection, replication and reactivity in host cells.</p>


Assuntos
Humanos , Citoesqueleto de Actina , Metabolismo , Actinas , Genética , Antígenos Virais , Células Cultivadas , Citomegalovirus , Citoesqueleto , Metabolismo , Embrião de Mamíferos , Fibroblastos , Metabolismo , Virologia , Proteínas Imediatamente Precoces
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