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OBJECTIVE: To isolate and purify the reference compounds and improve the quality standard of Korean medicinal Herba Artemisiae sacrorum. METHODS: The chemical constituents were isolated from the aerial parts of Korean medicinal herb Artemisia sacrorum by silica gel, ODS column chromatography and preparative HPLC, and the structures were identified by NMR and MS. RESULTS: Fourteen known compounds were isolated and identified as follows:1α-acetoxyeudesm-4-en-6β,11βH-12,6-olide(1),(11S)-3-oxoeudesma-1,4(15)-dieno-12,6α-lactone(2), 1-epi-dehydroisoerivanin(3), 11-epi-taurin(4), chrysanthemolide(5), 1α-acetyl-gallicadiol(6), erivanin(7), 1α, 4α-dihydroxyeudesm-2-en-5α, 6β, 11βH-12, 6-olide(8), vulgarin(9),(+)-dehydrovomifoliol(10), isoevodionol(11),(+)-epi-pinoresinol(12), lariciresinol-4'-O-β-D-glucopyranoside(13), and lariciresinol-4-O-β-D-glucopyranoside(14). CONCLUSION: All compounds are obtained from this plant for the first time. These compounds can be used as reference substances for the quality control of this ethnic medicine.
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OBJECTIVE: To establish a quantitative method for determining the contents of uracil, cytidine, hypoxanthine, xanthine, uridine, inosine, and guanosine in Holotrichia diomphalia Larvae by HPLC. METHODS: The HPLC analysis was performed on Waters HSS T3 column(4.6 mm×250 mm, 5 μm). The mobile phase was composed of acetonitrile(A) and water(B), and gradient elution was carried out. The flow rate was 1.0 mL•min-1. The column temperature was maintained at 30 ℃. The detection wavelength was 260 nm. RESULTS: The correlation coefficients of the seven components ranged from 0.999 1 to 1.000 0. The average recovery rates(n=6) were between 91.2% and 97.4%, and the RSDs were between 1.5% and 2.3%. CONCLUSION: The proposed method is simple, accurate and reliable, thus providing basis for comprehensive quality control of Holotrichia diomphalia Larvae.
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The TLC method was established for identification of Holotricha diomphalia larvae and the HPLC method was used to determine the content of inosine and guanosine in H. diomphalia larvae. The HPLC analysis was performed on a Waters HSS T3（4.6 mm×250 mm, 5 μm） column of with mobile phase consisting of acetonitrile (A) and 0.08% trifluoroacetic acid (B) in gradient elution. The detection wavelength was 260 nm. The flow rate was 1.0 mL·min⁻¹. The column temperature was 30 °C. As a result, TLC identification method had a good reproducibility and highly specificity. The linear equations of inosine and guanosine were in good linear range (r>0.999 8). The average recovery of inosine and guanosine was 96.53% (RSD=1.6%), 99.71% (RSD=2.7%). The method is simple, accurate and reproducible, which can provide a basis for quality standard improvement H. diomphalia larvae.
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This paper reported the suitable medium of L S-1 stain in detail,which could yield natural blue pigment.Single-factor exper imental design shows that the best carbon source was 2% glucose and nitrogen was 0 1% KNO 3.Orthogonal experimental design shows that the most suitable fermen tation medium was consisted of 4% glucose,0 1% KNO 3,0 075% salt and 10?g/m L FeSO 4.The best cultivation temperature was 30℃ and pH7 4.The dissolved oxyg en on the process of fermentation,as well as the variety of pH and the utilized condition of carbon and nitrogen were measured and analyzed.The separation of th is blue pigment by HPLC shows that this material contains actinorhordin and at l east other four ingredients.