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BACKGROUND: In our previous study, we prepared some silk fibroin/polyactic acid composite materials at different proportions, which are verified as tissue-engineered materials with good performance by systemic hypersensitivity test, acute toxicity test, hemolysis test, pyrogenic test, and cell co-culture experiment. OBJECTIVE: To study the subcutaneous degradation of silk fibroin/polylactic acid composite materials at different proportions in mice. METHODS: Fifty-four ICR mice were subjected to subcutaneous cystic clearance on the both sides of the spine, and then randomized into three groups. The silk fibroin/polylactic acid composites at 40:60, 50:50, and 30:70 were implanted into the cystic space, respectively. The specimens were taken out at 1, 3, 9 weeks after implantation. Histopathological staining, Masson staining, and CD34 immunohistochemical staining were performed. RESULTS AND CONCLUSION: (1) Hematoxylin-eosin staining: At 9 weeks after implantation, new blood vessels gradually formed and visible fibroblasts gradually grew into the composites in the three groups. Structural collapse of the materials became more apparent, and tissues and cells further grew into the materials. The 40:60 and 50:50 composites were degraded more quickly than the other one. (3) Masson staining: At 9 weeks after implantation, mature blood vessels and collagen fibers were detectable in the three groups, which were more apparent in the 40:60 and 50:50 groups than the 30:70 group. The collagen fiber expression in the 50:50 group was significantly higher than that in the other two groups at different time after implantation (P < 0.05). (3) CD34 immunohistochemical staining: At 1-3 weeks after implantation, CD34 positive expression gradually increased in the three groups. After 9 weeks, the CD34 expression decreased in the three groups; however, the CD34 positive expression in the 50:50 group was significantly higher than that in the other two groups at different time after implantation (P < 0.05). To conclude, the silk fibroin/polylactic acid composite material at a proportion of 50:50 was degraded more quickly in mice.
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<p><b>OBJECTIVE</b>To explore the feasibility and value of detecting CyclinD1 and BCL-2 in patients with B-cell lymphoma by using flow cytometry.</p><p><b>METHODS</b>Fifty-three patients with lymphoma were selected, and 50 healthy persons in the same period were selected as control. The expression levels of CyclinD1 and BCL-2 in patients with various subtypes of lymphoma were detected by using flow cytometry (FCM).</p><p><b>RESULTS</b>When the dilution time was 1 min and the dilution proportion was 1:20, the cell morphology was the best complete, at the 4 min the cell morphology was best status. The mean fluorescence intensity of CyclinD1 and BCL-2 between persons of control group and patients with B-cell lymphoma showed significant difference, the CyclinD1 level (1.824 ± 0.315) and BCL-2 levels (4.257 ± 0.528) of patients with B-cell lymphoma were obviously higher than the CyclinD1 level (0.634 ± 0.153) and BCL-2 level (1.926 ± 0.328) of persons in control group, the CyclinD1 and BCL-2 expression levels of patients with HL were significantly lower than CyclinD1 and BCL-2 levels of patients with NHL (P < 0.01). After treatment, the expression levels of CyclinD1 and BCL-2 in patients with B lymphoma were significantly lower than these befor treatment.</p><p><b>CONCLUSION</b>Using the method of flow cytometry for detecting CyclinD1 and BCL-2 expression levels in lymphoma cells of patients is feasible, and it can be applied clinically to evaluate the treatment efficacy.</p>