RESUMO
Indole-3-carbinol(I3C),an important anticancer compound found in broccoli,has attracted considerable attention.The rapid extraction and accurate analysis of I3C in the pharmaceutical industry in broccoli is challenging as I3C is unstable at low pH and high temperature.In this study,a rapid,accurate,and low-cost ultrasound-assisted dispersive-filter extraction(UADFE)technique based on poly(deep eutectic solvent)-graphene oxide(PDES-GO)adsorbent was developed for the isolation and analysis of I3C in broccoli for the first time.PDES-GO with multiple adsorption interactions and a fast mass transfer rate was synthesized to accelerate adsorption and desorption.UADFE was developed by combining dispersive solid-phase extraction(DSPE)and filter solid-phase extraction(FSPE)to realize rapid extraction and separation.Based on the above two strategies,the proposed PDES-GO-UADFE method coupled with high-performance liquid chromatography(HPLC)allowed the rapid(15-16 min),accurate(84.3％-96.4％),and low-cost(adsorbent:3.00 mg)analysis of I3C in broccoli and was superior to solid-phase extraction,DSPE,and FSPE methods.The proposed method showed remarkable linearity(r=0.9998;range:0.0840-48.0 μg/g),low limit of quantification(0.0840 μg/g),and high precision(relative standard deviation≤5.6％).Therefore,the PDES-GO-UADFE-HPLC method shows significant potential in the field of pharmaceutical analysis for the separation and analysis of anti-cancer compounds in complex plant samples.
RESUMO
Objective To develop an automatic chest X-ray protective device to decrease the harm to the patient during Xray examination.Methods Mobile lead plate was involved in to protect the non-target organs and tissues.The device had the frame made of 304 stainless steel,and the core protective part of lead plate with 5 mm-thickness,whose components included noiseless motor,transmission system and lithium battery.Results The device decreased the exposure field and radiation dosage,and thus contributed to the patient protection effectively.Conclusion The device reduces the exposure dosage greatly,and is of great value for enhancing efficacy.
RESUMO
Objective To discuss the diagnostic value of Matrix metalloproteinase-9 (MMP-9) and Epidermal growth factor (EGF) in uterine myoma.Methods Seventy-three patients with uterine leiomyoma treated by surgery as treatment group,and 65 healthy women were selected as control group.The levels of MMP9 and EGF in both groups were detected by enzyme-linked immunosorbent assay method.Results The levels of MMP-9and EGF in treatment group were significantly higher than those of control group((1.72±0.36) mg/L vs.(1.28±0.25) mg/L, (342.16± 128.65) ng/L vs.(209.84± 100.34) ng/L;t =8.241,6.678;P ＜0.01).There were no relationships between the levels of MMP-9 and EGF with maximum diameter of uterine myoma (P ＞0.05).Conclusion Serum levels of MMP-9 and EGF have some clinical value in diagnosing uterine leiomyoma.
RESUMO
Directive 2004/24/EC of the European Parliament and the Council entered into force on April 30th, 2004. After 7 years, there is no Chinese medicine to be registered successfully in European market as traditional herbal medicinal products. The thesis gives some ideas to tackle this problem. Procedure for the Preparation of Community Monographs for Traditional Herbal Medicinal Products (EMEA/HMPC/182320/2005) published by European Medicines Agency is an important guidance for traditional herbal products to enter European Community monographs. The thesis introduces and details the procedure as well as gives feasible suggestions about the procedure. It suggests that Chinese medicines enter European Community monographs first, and then apply the registration according to the directive 2004/24/EC. This is an easier access to European market.
RESUMO
<p><b>OBJECTIVE</b>To investigate DPPH radical scavenging activities of 31 flavonoids and phenolic acids and 10 extracts of Chinese materia medica.</p><p><b>METHOD</b>The antioxidant activities of the above samples were evaluated by a DPPH method, the half-wave oxidation potentials (E1/2) of the 31 compounds were determined by an HPLC-CoulArray method, at the same time, phenolic contents of the the total compounds in the 10 extracts of Chinese materia medica were analyzed by Folin-Ciocalteu method.</p><p><b>RESULT</b>The 31 compounds showed a 50% inhibition of DPPH radical in the concentration range of 6.7-3 500 micromol x L(-1), in which (-) -EGCg demonstrated the strongest activity with the IC50 value of 6.7 micromol x L(-1). The E1/2 of 31 compounds spanned a wide potential range of more than 0.6 V. Myricetin had the lowest E1/2 value (20 mV) whereas apigenin and vitexin-2"-O-rhamnoside had the highest E1/2 value (620 mV). Among the 10 herb extracts, having 82% phenolic acid, tea extract showed the strongest DPPH radical scavenging activity with the IC50 value of 0.011 7 mg x mL(-1) whereas safflower demonstrated the weakest DPPH radical scavenging activity with the IC50 value of 1.250 mg x mL(-1), in which only 7% phenolic acids was tested.</p><p><b>CONCLUSION</b>The DPPH radical scavenging activities of the 31 compounds were founded to be related to their chemical structures, such as the number and position of hydroxyl groups. And a qualitative relationship was found between DPPH radical scavenging activities and E1/2 values of the 31 compounds, the lower the E1/2 values, the higher the DPPH radical scavenging activities. A quantitative relationship was obtained to describe the DPPH radical scavenging activity of the herb extracts: Y = 7.779X(-0.48), r = 0.929 5, where Y stands for the concentration for 50% inhibition of DPPH radical, and X stands for the concentration of total phenolic compaunds, namely the extracts with higher content of flavonoids and phenolic acid exhibited the stronger DPPH radical scavenging activity.</p>