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Objective To explore the effect of compound active tea of Lithocarpus litseifolius on uric acid levels and kidney function of mice with hyperuricemia nephropathy and to provide an experimental basis for the development of hyperuricemia nephropathy drugs and functional food.Methods A mouse model of hyperuricemia nephropathy was established by administering potassium oxazinate with adenine.Mice were randomly divided into common,model,positive drug(10 mg/(kg·d))and compound active tea of Lithocarpus litseifolius high-,middle-and low-dose groups(10 g/(kg ·d),3.33 g/(kg·d)and 1.11g/(kg·d),respectively).One hour after the last gavage,urine protein(UP)was measured by CBB method,urea nitrogen(UUN)was measured by urease method.Orbital blood pampling,blood was collected for uric acid(UA)analysis by enzyme ratio method,urea nitrogen(BUN)was measured by urease method.The serum contents of interleukin 6(IL-6)and tumor necrosis factor(TNF-α)were measured by ELISA.Take kidney tissue,levels of urate transporter 1(URAT1)and glucose transporter 9(GLUT9)were measured by quantitative fluorescence,kidney histopathological changes were observed by HE stainning.Results Compared with the control group,the model group's levels of UP,UUN,UA,BUN,IL-6,URAT1,ULUT9 and TNF-α were significantly increased(P＜0.01,P＜0.05),and the renal tissue structure was normal.Compared with the model group,the positive group's levels of UP,UUN,UA,BUN,IL-6 and TNF-α were significantly decreased(P＜0.01,P＜0.05),there was little glomerular atrophy or deformation in the kidneys,kidney tubular dilatation was occasionally seen,but there was no inflammatory cell infiltration.Compared with the model group,the high-dose compound active tea of Lithocarpus litseifolius group's UP,UUN,UA,BUN,IL-6,URAT1,TNF-α and GLUT9 levels were significantly decreased(P＜0.01,P＜0.05).The middle-dose compound active tea of Lithocarpus litseifolius group's UP,UUN,UA content,IL-6,URAT 1,GLUT9,BUN and TNF-αwere significantly decreased(P＜0.01,P＜0.05).The low-dose compound active tea of Lithocarpus litseifolius group's UP,UUN,UA,IL-6,URAT1,BUN,TNF-α and GLUT9 levels were significantly decreased(P＜0.01,P＜0.05).Conclusions Compound active tea of Lithocarpus litseifolius can reduce uric acid in mice with hyperuricemia nephropathy and has a certain protective effect on the kidneys.The mechanism may be related to the inhibition of uric acid reabsorption,and the specific mechanistic details should be further investigated.
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Objective:To investigate the current status of radiodiagnostic and radiotheraputic resources and the frequency of medical radiation exposure in order to provide basic data for reasonably allocating medical resource and standardizing meical exposure.Methods:According to data available from the Jiangsu Radiation Health Information Management Platform in 2020 and the total number of permanent residents from Wuxi Statistical Yearbook 2019, a statistical analysis was carried out of the staff and equipment and the frequency of medical radiation exposure in Wuxi mecical institutions.Results:There were 923 items of radiodiagnostic and radiotheraputic equipment, 2 480 medical radiation workers in Wuxi city in 2020, which were estimated to be 140.03 items of equipment and 376.24 workers per 10 6 population. The frequency of X-ray diagnosis and treatment were 1 137.53 procedures per 10 3 population, including 646.17 per 10 3 population for conventional X-ray diagnosis, 394.11 per 10 3 population for CT scan, 5.34 per 10 3 population for interventional radiology, 0.79 per 10 3 population for radiotherapy and 7.12 per 10 3 population for nuclear medicine. Interventional radiology, radiotherapy and nuclear medicine was mainly concentrated in tertiary hospitals. Conclusions:The number of radiodiagnostic and radiotheraputic resources in Wuxi is above the national and provincial level, although in a unbalanced state. Conventional X-ray diagnosis frequency in Wuxi is higher than that of provincial level, resultsing in higher collective dose. Radiological diagnosis and treatment activities needs a approach to the process of justification.
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Objective To study gross α and gross β radioactivity in environmental samples in Wuxi, China, and perfect the radioactivity data, and to discover possible radioactive contamination in time. Methods The BH1227 low-background α and β measuring instrument was used to determine gross α and gross β radioactivity in sedimentary dust, aerosol, drinking water, and food samples in Wuxi. Results Among environmental samples collected in Wuxi, 2014—2019, the mean gross α and gross β radioactivity levels were 0.01～5.98 Bq/kg and 9.47～547.86 Bq/kg in food samples, respectively, 1.2～22 mBq/L and 0.1～141 mBq/L in water samples, respectively, 39～44790 mBq/m3 and 743.1～101500 mBq/m3 in sedimentary dust samples, respectively, and 50～1125 μBq/m3 and 38～9510 μBq/m3 in aerosol samples, respectively. Conclusion We should strengthen radioactivity monitoring of environmental samples in Wuxi through increasing the scope and frequency of monitoring, to perfect baseline radioactivity data of Wuxi.