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Objective:To study expressions of netrin-1 and its receptor UNC5C in female precocious puberty rat hypothalamus, and explore its effect on precocious puberty process.Methods:Forty female one-week-old SD rats were randomly divided into four groups: experimental group A (precocious puberty early youth), experimental group B (precocious puberty medium youth), group A (normal pre-puberty), group B (normal early youth) with 10 rats in each group. Precocious puberty experimental rats were induced with Danazol and rats in control group were injected with saline. Uterus and ovaries were removed, specimens were weighed, uterus index and ovarian index were calculated, and amount of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) were detected from the blood by ELISA. Real-time PCR was used to detect netrin-1 and its receptor UNC5C, as well as hypothalamic gonadotropin-releasing hormone (GnRH) mRNA expression in hypothalamus tissues; and then, a co-immunoprecipitation study of interactions between netrin-1 and its receptor UNC5C was carried out.Results: Relative target gene expression levels of control group A, control group B, experimental group A, and experimental group B (withβ-actin as an internal control for normalization) were as follows: Netrin-1: 3.5±0.9, 5.4±0.7, 4.9±1.0, 5.3±0.3; UNC5C: 0.8±0.04, 1.7±0.2, 1.82±0.23, 1.58±0.4; GnRH: 1.2±0.3, 2.7±0.3, 2.4±0.7, 3.2±0.4.Conclusions:LH and FSH concentrations, netrin-1 and its receptor expression are increased in precocious puberty animal models.
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Objective To observe effects of tripterygium glycosides combined with Xiangdan injection in henoch schonlein purpura nephritis (HSPN)and influence of the clotting mechanism,providing reference for clinical diagnosis and treatment.Methods 76 cases of children with allergic purpura nephritis were randomly divided into control group(n=38)and observation group(n=38)according to the random number table.Control group was given Xiangdan injection treatment.Observation group was given Xiangdan injection treatment combined with tripterygium glycoside.12 weeks was considered as a course of treatment. Results After treatment,the total efficiency rate in treatment group(94.74%)was significantly higher than control group(68.42%),which was statistically different(P <0.05).Before treatment,prothrombin time(PT),fibrinogen(FIB),activated partial thromboplastin time(APPT),platelet(PLT)levels were not significantly different between two groups;FIB and PLT levels in the control group were significantly lower than before treatment(P<0.05 ),PT and APPT had no statistically significant difference before and after treatment.After treatment in observation group,FIB and PLT levels were significantly lower than before treatment(P<0.05 ),PT level was significantly higher compared with before treatment(P<0.05 ),APTT level had no difference before and after treatment.After treatment,PT in treatment group was significantly higher than the control group(P<0.05 ),PLT in treatment group were significantly lower than the control group (P <0.05 ),FIB and APTT had no statistical difference.There was no adverse reaction in two groups after treatment.Conclusion Tripterygium glycosides combined with Xiangdan injection treatment for HSPN has significant effect,which affect the children clotting mechanism to improve the PT,FIB,PLT levels.It is safe and valuable in clinical research.