RESUMO
The responsibility of root is absorbing water and nutrients, it is an important plant tissue, but easily to be affected by biotic and abiotic stresses, affecting crop growth and yield. The design of a synthetic root-specific promoter provides candidate promoters for the functional analysis and efficient expression of stress-related genes in crop roots. In this study, a synthetic root-specific module (pro-SRS) was designed using tandem four-copies of root specific cis-acting elements (OSE1ROOTNODULE, OSE2ROOTNODULE, SP8BFIBSP8AIB, and ROOTMOTIFAPOX1), and fused with minimal promoter from the CaMV 35S promoter to synthesize an artificially synthetic SRSP promoter. The SRSP promoter was cloned in pCAMBIA2300.1 by replacing CaMV 35S promoter so as to drive GUS expression. The constructs with SRSP promoter were transformed in tobacco by Agrobacterium-mediated method. SRSP promoter conferred root-specific expression in transgenic tobacco plants through Real-time PCR (RT-PCR) analysis and GUS histochemical staining analysis. It is indicated that the repeated arrangement of cis-acting elements can realize the expected function of the promoter. This study laid a theoretical foundation for the rational design of tissue-specific promoters.
Assuntos
Agrobacterium , Genética , Clonagem Molecular , Regulação da Expressão Gênica de Plantas , Raízes de Plantas , Genética , Plantas Geneticamente Modificadas , Regiões Promotoras Genéticas , Genética , Estresse Fisiológico , Nicotiana , Genética , Transformação GenéticaRESUMO
The article analyses the current situation of the unbalanced development of Chinese higher medical education and the reasons for it,and points out that to promote its development,gradation and diversify development of education must be realized.