Incidence of unintended pregnancy within 2 years after delivery and its influencing factors in China / 中华妇产科杂志

Objective:To investigate the present situation of unintended pregnancy within two years postpartum and its influencing factors in China.Methods:Participants who delivered a live birth at 60 hospitals in 15 provinces in the eastern, central and western regions of China during July 2015 to June 2016 were interviewed by using structured questionnaire. Information on occurrence of unintended pregnancy within 2 years after delivery, postpartum contraceptive use, sexual resumption, breastfeeding, and women′s socio-demographic characteristics, and so on, were collected. Life-table analysis, cluster log-rank tests and a 2-level Cox regression model were used for data analysis.Results:A total of 18 045 postpartum women were investigated. The cumulative 1- and 2-year unintended pregnancy rates after delivery were 5.3% (95% CI: 4.5%－6.1%) and 13.1% (95% CI: 11.3%－14.8%), respectively. Cox regression model analysis showed that the risk of unintended pregnancy within 2 years postpartum were increased in younger women, ethnic minorities, women with abortion history, and those who had a vaginal delivery with short lactation time and late postpartum contraceptive initiation (all P<0.01). The risk of postpartum unintended pregnancy was not associated with geographic regions and hospitals where women gave a birth (all P>0.05). Conclusions:In China, the risk of unintended pregnancy within 2 years after delivery is relatively high. Service institutions and service providers should improve the quality of postpartum family planning services, promote the use of high effect contraceptive methods, and educate women to use a method at the time of their sexual resumption or even before.

Hypothermic machine perfusion promotes the regeneration of isolated liver / 中华器官移植杂志

Objective To study the effects of different preservation methods on the isolated liver injury and regeneration.Methods Twelve Sprague-Dawley rats were randomly assigned to two groups:static cold storage (SCS) and hypothermic machine perfusion (HMP) (n =6 each).Histidinetryptophan-ketoglutarate solution (HTK) was used as preservation solution.The graft was preserved in HTK (4 C) for 6 h in SCS group,and that was perfused using HTK in HMP group.Liver tissue was obtained and fixed in 10％ neutral formalin for immunohistochemistry.The fresh liver tissue was collected and stored in-80℃ for RT-PCR and Western blotting analyses.Results Compared to SCS,HMP improved liver regeneration ability in terms of PCNA and ki67 detection and promoted cell proliferation in PCR levels (Cdc25A,cdk1,cdk6) and Western blotting levels (Cyelin D1,Cyclin E1).Conclusion HMP is superior to SCS in liver regeneration,and expected to be the ideal method for preservation of donor liver.

Hypothermic machine perfusion promotes the regeneration of isolated liver / 中华器官移植杂志

Objective To study the effects of different preservation methods on the isolated liver injury and regeneration.Methods Twelve Sprague-Dawley rats were randomly assigned to two groups:static cold storage (SCS) and hypothermic machine perfusion (HMP) (n =6 each).Histidinetryptophan-ketoglutarate solution (HTK) was used as preservation solution.The graft was preserved in HTK (4 C) for 6 h in SCS group,and that was perfused using HTK in HMP group.Liver tissue was obtained and fixed in 10％ neutral formalin for immunohistochemistry.The fresh liver tissue was collected and stored in-80℃ for RT-PCR and Western blotting analyses.Results Compared to SCS,HMP improved liver regeneration ability in terms of PCNA and ki67 detection and promoted cell proliferation in PCR levels (Cdc25A,cdk1,cdk6) and Western blotting levels (Cyelin D1,Cyclin E1).Conclusion HMP is superior to SCS in liver regeneration,and expected to be the ideal method for preservation of donor liver.

Prevalence of Panton-Valentine leukocidin genes and antimicrobic resistance in Staphylococcus aureus isolates from outpatients with skin and soft-tissue infections in Wuhan city / 中华皮肤科杂志

Objective ToestimatetheprevalenceofPanton-Valentineleukocidin (PVL)genes and antimicrobial resistance in methicillin-sensitive Staphylococcus aureus (MSSA)isolateds from outpatients with skin and soft-tissue infections (SSTIs)in Wuhan city. Methods A total of 182 MSSA isolates were collected from outpatients with SSTIs in 5 different hospitals in Wuhan city between 2011 and 2013. The Kirby-Bauer′s disk diffusion method was used to evaluate antimicrobial susceptibility of the MSSA isolates, and multiplex PCR was performed to detect mecA and PVL genes in these isolates. Results Of the 182 MSSA isolates, 65 (35.71%)carried PVL genes. The positive rate of PVL genes was significantly different among patients with different diseases (χ2 = 49.76, P = 0.00), and relatively higher in patients with furuncles/carbuncles(7/7), folliculitis(3/3), abscesses(55.53%, 30/57)or impetigo(2/4). The age of patients with PVL-positive MSSA infection was significantly younger than that with PVL-negative MSSA infection (35.40 ± 19.31 years vs. 43.21 ± 20.75 years,t = 2.50, P = 0.01). Among 65 PVL-positive MSSA isolates, the rate of resistance to clindamycin was highest (87.69%), followed by that to penicillin(53.85%)and erythromycin(41.54%). The frequency of resistance to clindamycin was highest in 117 PVL-negative MSSA isolates, followed by that to penicillin (20.51%)and ampicillin (12.82%). Furthermore, there was a significant increase in the rate of resistance to penicillin(χ2 = 21.19), ampicillin(χ2 = 97.97), doxycycline(χ2 =11.61), ciprofloxacin(χ 2 = 8.07), erythromycin(χ 2 = 25.04)and gentamicin(χ 2 = 10.86)in PVL-positive MSSA isolates compared with PVL-negative MSSA isolates (all P < 0.05). Conclusions MSSA isolates from outpatients with SSTIs in Wuhan city are resistant to most β-lactam antibiotics. Flucloxacillin, compound sulfamethoxazole tablets or doxycycline is recommended for empirical treatment of PVL-positive MSSA infections.

EFFECTS OF NURSING INTERVENTION ON THE REHABILITATION OF PATIENTS WITH BENIGN PAROXYSMAL POSITIONAL VERTIGO / 现代医院

Objective To investigate the effects of nursing intervention on the rehabilitation of patients with benign paroxysmal positional vertigo (BPPV).Methods 64 BPPV patients were randomly divided into control group and observation group, with 32 cases in each group.The control group received otorhinolaryngology routine nursing, while the observation group was managed with continuously systemic nursing interventions, including manipulative re-duction, psychological nursing intervention, health education, head nursing, follow -up and family nursing interven-tion.The Hamilton Anxiety Scale (HAMA) and Hamilton Depression Rating Scale (HDRS) were applied to evaluate the intervention effects.Results Cure rate of the observation group was significantly higher than that of the control group, and recurrence rate of the observation group was obviously lower than that of the control group(p <0.05).The anxiety and depression assessment of the observation group was significantly lower than that of the control group, with statistical difference (p <0.05).Conclusion Systemic nursing intervention can effectively improve the rehabilita-tion of patients with BPPV, and reduce recurrence rate.

Clinical distribution and antimicrobial resistance of Acinetobacter bau-mannii isolated from patients in consecutive three years / 中国感染控制杂志

Objective To analyze the clinical distribution and antimicrobial resistance of Acinetobacter baumannii (A.baumannii)from a hospital,so as to guide the rational use of antimicrobial agents and provide scientific basis for the prevention and control of healthcare-associated infection(HAI).Methods Distribution and antimicrobial susceptibility testing results of A.baumannii isolated from patients between January 2011 and December 2013 were analyzed retrospectively.Results A total of 339 A.baumannii isolates were isolated within three years,291 iso-lates (85.84%)were from sputum,followed by urine (n=29,8.55%),secretion(n=12,3.54%)and blood (n=3,0.89%).The main distribution departments were intensive care unit(ICU,n=149,43.95%),department of re-spiratory medicine (n=46,13.57%),department of pediatrics (n =32,9.44%)and oncology (n =22,6.49%). The resistant rate of A.baumannii to tobramycin and imipenem were 11 .11 %-49.47%,the resistant rates to the other antimicrobial agents were relatively high;antimicrobial resistant rate of 2012 and 2013 were obviously lower than 2011 .Conclusion A.baumannii from this hospital are mainly from sputum specimens,and mainly distribute in ICU and department of respiratory medicine,antimicrobial resistant rate is high,but there is a decreasing tenden-cy in recent years.

Preparation and physicochemical characterization of hydrogel microspheres for embolization / 北京大学学报(医学版)

Objective:To develop and characterize the hydrogel microspheres for embolization.Methods:N-[tris(hydroxymethyl)methyl] acrylamide-gelatin microspheres(TGMs)were prepared by an inverse suspension polymerization approach.Effects of materials on size,water absorption rate and elasticity of the microspheres were investigated.The materials which were included consisted of gelatin in the range of 10.0-100.0 g/L,N-[tris(hydroxymethyl)methyl]acrylamide in the range of 33.3-200 g/L,cross-linking agent N,N'-methylene-bis-acrylamide in the range of 3.3-10.0 g/L,surfactant Span 80 in the range of 0.5-1.8 g/L,and initiator ammonium persulfate in the range of 1.0-5.0 g/L.The appearance of TGMs was observed under microscope.TGMs were analyzed by infrared spectrum(IR).Results:The TGMs were round with smooth surface in view of photograph of microscope.The average diameter of TGMs was increased with the increase of gelatin,monomer or cross-linking agent concentrations but decreased with the increase of surfactant or initiator Concentration.The water adsorption rate of the microspheres was decreased with the increase of gelatin or cross-linking agent concentration but not affected by surfactant concentration.The elasticity of TGMs was increased with the increase of monomer or cross-linking agent concentration,decreased with the increase of gelatin concentration,but not affected by surfactant or initiator concentration.All factors above considered,the final prepared TGMs consisted of 10 g/L gelatin,100.0 g/L monomer,6.7 g/L cross-linking agent,0.9 g/L surfactant,and 3.0 g/L initiator.The average diameter of TGMs obtained was about 700.0 ?m.The water adsorption rate and the elasticity in accordance with the maximum diameter of the microspheres passed through a microcatheter of TGMs were 12.4(g/g),and 1 600.0 ?m,respectively.The results of IR spectra confirmed the polymerization of monomer,resulting in N-[tris(hydroxymethyl)methyl]acrylamide-gelatin microspheres.Conclusion:The developed TGMs seemed to be suitable for clinical embolization according to the surface,average diameter,elastic and hydrophilic property of TGMs.