RESUMO
OBJECTIVE: To study the effects of Tongbi Mixture 2, a compound traditional Chinese herbal medicine for treating rheumatoid arthritis (RA), on immunoregulation of T lymphocytes in rats with collagen-induced arthritis (CIA). METHODS: Forty Wistar rats were randomly divided into normal control group, untreated group, Tongbi Mixture 2-treated group, methotrexate (MTX)-treated group and Tripterygium wilfordii polyglycoside (TWP)-treated group. Except for the rats of the normal control group (injection with normal saline), rats of the other four groups were subcutaneouly multipoint-injected with collagen protein II to induce CIA. The rats were treated with normal saline, Tongbi Mixture 2, MTX tablets and TWP tablets respectively for 36 days. The expressions of CD28 and CD152 were detected by flow cytometry, while the content of tumor necrosis factor-alpha (TNF-alpha) was analyzed by enzyme-linked immunosorbent assay. RESULTS: The expression of CD28 among the three drug treated groups had no statistical difference (P>0.05), while significantly higher than that of the normal control group (P0.05). There were no statistical differences in content of TNF-alpha between the drug treated groups and the normal control group (P>0.05), but the content of TNF-alpha of the drug treated groups was lower than that of the untreated group (P<0.05 or P<0.01). CONCLUSION: Tongbi Mixture 2 can inhibit T lymphocytes through down-regulating the expressions of CD28 and CD152 and the content of TNF-alpha, which may be the major mechanisms in treating RA.
RESUMO
Objective To observe the therapeutic effect of Yingqiling for Graves disease(GD)and to explore its therapeutic mechanism.Methods Eighty GD patients were equally randomized into two groups.The treatment group received oral use of Yingqiling Tablets(mainly composed of Radix Pseudostellafiae,Radix Astragali,Radix Ophiopogonis,Fructus Schisandrae Chinensis,Spica Prunellae,Semen Ziziphi Spinosae,Concha Ostreae, Bulbus Fritillariae Thunbergii,Radix Ranunculi Ternati,Radix Scrophulariae,Radix Paeoniae Rubra and Thiamazole 1 mg),and the control group received Thiamazole Tablets.After treatment for 2 months,the therapeutic effect was evaluated and the changes of serum levels of thyroglobulin antibodies(TGAb),thyroid peroxidase antibody(TPO-Ab),and the apoptosis-associated factors of soluble Fas(sFas)and bcl-2 were observed before and after treatment.Results Twenty-one patients were clinically effective,13 markedly effective,6 effective,0 ineffective,and the total effective rate was 100% in the treatment group;14 patients were clinically effective,13 markedly effective,7 effective,6 ineffective,and the total effective rate was 85.0% in the control group.The effect in the treatment group was superior to the control group(P