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Objective Comprehensively considering the effectiveness and safety of massage, a method for evaluating the pros and cons of oscillation excitation and pulse excitation for lumbar oblique-pulling manipulation was proposed, and lumbar oblique-pulling manipulation under oscillation excitation was optimized. Methods A multi-rigid body biomechanical model of thoracolumbar spine was established. The manipulation force was used as the input force of the model. Using MATLAB/Simulink, variation of the displacement and acceleration of each lumbar segment with time was simulated. For the optimization of lumbar massage manipulation, the core elements of massage force, namely, frequency (f) and operand (n) were changed, and then the maximum relative displacement and maximum acceleration of each lumbar segment were compared. A new index z was proposed to comprehensively evaluate effectiveness and safety of the manipulation. Results The maximum relative displacement of each lumbar segment was almost equal when lumbar oblique-pulling manipulation under two kinds of excitation was applied. For lumbar oblique-pulling manipulation, the maximum acceleration of each lumbar segment under oscillation excitation was significantly smaller than that under pulse excitation. When the frequency of massage was 1-2.5 Hz, the overall effect of massage was better, and the overall effect had no relation with the operands, and the force of massage lasted for one operand; when the frequency of message was 3.33 Hz and the operands were more than 5, the massage had the best effect, meanwhile the strength of each lumbar segment was relatively large; when the frequency of massage exceeded 5 Hz, and the overall performance of massage was not good. Conclusions Lumbar oblique-pulling manipulation under oscillation excitation is safer than that under pulse excitation. The research findings provide doctors with a reasonable range of operating parameters for lumbar oblique-pulling manipulation under pulse excitation.
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To investigate the detection threshold of Treponema pallidum specific antibody method by chemiluminescent immunoassay (CLIA) in Siemens ADVIA Centaur XP for Syphilis serological test, and compare with the results derived from CMIA, TP-WB and TPPA method. The result can serve as reference for the application of CLIA. In total 30 887 samples screened by Treponema pallidum specific antibody method were collected by Abbott architect i2000 CMIA from July 2018 to July 2019 in Yanda Hospital of Hebei Province. We selected 153 patients with the ratio of sample absorbance to critical value (S/CO) of 1-9 by CMIA screening of Treponema pallidum specific antibody as the research objects. The reverse sequence of syphilis serological detection was adopted, and TP-WB and TPPA were used as the confirmation methods respectively. MedCalc software was used to analyze the results of ROC curve, and the cut-off value was obtained. Chi square test was used to test the difference significance of counting data. The detection results of Treponema pallidum specific antibody in the same batch of serum samples were unequal by different methods. There was no significant difference between CLIA method and TPPA method, but significant difference between CLIA method with TP-WB method and CMIA method was found. TPPA test results and TP-WB test results were taken as gold standards, ROC curve analysis showed that the best diagnostic cutoff value of CLIA method was 4.01 and 16.06, respectively, and the area under the curve was 0.961 and 0.838. The suggested cutoff value of CLIA method is quite different when using different syphilis serological test methods as the gold standard, Therefore, when the S/CO value determined by CLIA is between 1.00 to 16.06, TP-WB method should be recommended as the first choice in laboratory serological test for recheck and confirmation to avoid clinical misdiagnosis.
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A novel method for quantification of astragaloside IV in Radix Astragli was developed by using online SPE liquid chromatography coupled with corona charged aerosol detection( CAD) . The sample solution was loaded into Acclaim Polar AdvantageⅡC18(50 mm×4. 6 mm, 3 μm) which was selected as online SPE column. Then the cleaning process was done by using the Right one of dual gradient pumps with Methanol-water as mobile phase. Acclaim C18(150 mm×4. 6 mm, 5 μm) was selected as analytical column with acetonitrile-water as mobile phase at a flow rate of 1. 0 mL/min. The eluate containing the target from SPE column was transferred into the analytical column by heart-cutting mode. The temperature of nebulizer of corona CAD was set at 30 ℃ and the nitrogen pressure was 241. 3 kPa. The baseline separation of astragaloside IV from matrix components has been achieved. There was a good linear correlation in the range of 4 . 0-80 mg/L for astragaloside IV and the correlation coefficient was 0 . 9998 . The standard addition average recovery of astragaloside IV in Radix Astragli was 97 . 6%. It has been validated that astragaloside IV in Radix Astragli and its preparations can be quantified rapidly and accurately with this method.
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Objective To observe the relationship between thyroid hormone levels and reduced pulmonary function in elderly male patients with chronic obstructive pulmonary disease(COPD).Methods One hundred and ninety-five cases of elderly male patients with COPD hospitalized from Jan 2006 to Feb 2010 were enrolled.Thyroid function and lung function(FEV1(forced expiratory volume in 1 second),FEV1/FVC(forced vital capacity))were examined routinely.Pulmonary function was compared between normal group(with normal thyroid function)and lower group(with thyroid hypofunction),thyroid function was also compared in patients with different grade levels(mild,moderate,severe,very severe)of pulmonary function.Results FEV1 and FEV1/FVC in thyroid hypofunction group were significantly decreased compared to the normal group(t =3.582,2.981,respectively,P ＜ 0.01).There was significant difference on T3,FT3 in patients with different grade levels of pulmonary function(F =1.959,1.873,respectively,P ＜ 0.05),among them,T3,FT3 were significantly different between mild group and severe group(P ＜ 0.05),but there was no significant difference in other groups(P ＞0.05).T4、FT4 、TSH displayed no significant difference in all groups(P ＞ 0.05).Conclusion Pulmonary function decreased obviously in elderly old male patients with COPD combined with thyroid hypofunction,and the lower level of thyroid hormone,the worse of pulmonary function.
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<p><b>OBJECTIVE</b>To establish a simple extraction, isolation and purification method for ginkgolide B from ginkgo leaf.</p><p><b>METHOD</b>The optimum conditions of extraction, isolation and purification were studied by taking the transfer rate of ginkgolide B as index.</p><p><b>RESULT</b>Ginkgo leaf was extracted with 70% ethanol for three times, the extracts were concentrated to remove ethanol and diluted by water till the crude drug density reached 0.1 g x mL(-1). The dilution was adsorbed with HPD-450 macroporous resin. The impurities were eluted with 20% ethanol and ginkgolide B was eluted with 80% ethanol. Then the 80% ethanol eluant was concentrated and crystallized. Finally the crude crystals were recrystallized with isopropanol. The purity of the ginkgolide B recrystallization was 95%.</p><p><b>CONCLUSION</b>The process was stable and easy to operate, which was suited to industrialized production.</p>
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Fracionamento Químico , Métodos , Cromatografia Líquida de Alta Pressão , Métodos , Medicamentos de Ervas Chinesas , Ginkgo biloba , Química , Ginkgolídeos , Lactonas , Folhas de Planta , QuímicaRESUMO
AIM: To study the effect of decreasing blood pressure on mechanical and heat pain sensitivity in spontaneously hypertensive rats.METHODS: 15 SHRs were intramuscularly given reserpine(1(?g?kg~(-1)?d~(-1))) on day 4-11.Blood pressure,paw withdrawal mechanical threshold(PWMT) and paw withdrawal thermal latency(PWTL) were measured every day.RESULTS: After the therapy of reserpine in spontaneously hypertensive rats,blood pressure decreased by 50 mmHg,mechanical and heat pain threshold were both decreased(P