Effect of low-level laser therapy on cytokines in gingival crevicular fluid of patients with severe periodontitis / 国际生物医学工程杂志

Objective:To study the effect of low-level laser therapy (LLLT) on cytokines in gingival crevicular fluid of patients with severe periodontitis, and to analyze the effect of LLLT on periodontal inflammation control and bone healing.Methods:A total of 43 teeth from 18 patients with severe periodontitis were selected. There were no differences in age, gender, and periodontal status of all patients enrolled. 20 teeth in the control group received basic periodontal treatment, and 23 teeth in the experimental group were received combination of basic periodontal treatment and LLLT. During the LLLT treatment, the gingival pocket was irradiated by low-level laser for 15 s on days 1, 2, and 7. One and four weeks after treatment, the gingival crevicular fluid the patients in the control group and the experimental group was collected, and the interleukin-1β (IL-1β), transforming growth factor-β (TGF), tumor necrosis factor-α (TNF-α), metal matrix protease-8 (MMP-8), soluble intercellular adhesion molecule-1 (sICAM-1) and periosteal protein levels were determined by enzyme-linked immunosorbent assay (ELISA).Results:After treatments, the levels of IL-1β, TNF-α, MMP-8 and sICAM-1 in the control group and the experimental group decreased, and the levels of TGF-β and periosteal protein increased. The basic periodontal treatment combined with LLLT had better therapeutic effect. One week after treatments, the differences in the levels of IL-1β and TNF-α between the two groups were statistically significant (all P<0.05). Four weeks after treatments, the differences in the levels of IL-1β, TNF-α, TGF-β, MMP-8 and sICAM-1 between the two groups were statistically significant (all P<0.05). Conclusions:LLLT treatment can effectively reduce the levels of IL-1β, TNF-α and MMP-8 in the gingival crevicular fluid of patients with severe periodontitis in a short period of time. LLLT has a certain effect on the recovery of periodontal tissue, including controlling periodontal inflammation, reducing the expression of sICAM-1 in gingival crevicular fluid, alleviating the symptoms of gum bleeding, promoting the secretion of TGF-β, increasing the level of periosteal protein, and inhibiting the destruction of periodontal tissue.

Clinical effect of low-level laser on severe periodontitis / 国际生物医学工程杂志

Objective:To evaluate the clinical effect of basic periodontal therapy combined with low-level laser irradiation on patients with severe periodontitis.Methods:18 patients with severe periodontitis (a total of 43 teeth samples) were enrolled from the Dental Department of Tianjin First Central Hospital from June 2018 to May 2019. The patients were averagely divided into the control group (20 teeth) and the experimental group (23 teeth) according to the random number table method. All the teeth were firstly received basic periodontal treatment. In the experimental group, the gingival pouch of the teeth was irradiated with low energy laser for 15 s on the 1st, 2nd, and 7th days after the basic periodontal treatment, in which the output power is 80 mw, the energy density is 4 J/cm 2, and irradiated area is 0.28 cm 2. Before the treatment and 1 week and 4 weeks after the treatment, the periodontal probing depth (PD), clinical attachment level (CAL), gingival sulcus bleeding index (SBI) of the teeth of the control group and the experimental group were detected, and the gingival sulcus of the two groups were collected at the same time. The level of basic fibroblast growth factor (b-FGF) in gingival crevicular fluid was detected by enzyme-linked immunosorbent assay (ELISA), and the level of serum hypersensitive C-reactive protein (hs-CRP) was detected by immunoturbidimetry. Results:Before the treatment, there were no significant differences in the age, PD, CAL, SBI, hs-CRP and b-FGF levels of patients with severe periodontitis between the two groups (all P>0.05). One week after the treatment, there was no statistically significant difference in PD, CAL, hs-CRP and b-FGF levels between the two groups (all P>0.05), and the SBI of the experimental group (2.43±0.97) was lower than that of the control group (4.13±0.78), and the difference was statistically significant ( P<0.05). Four weeks after the treatment, the SBI of the experimental group (2.26±0.96) was lower than that of the control group (3.75±0.72) ( P<0.01), there was no statistical difference between the two groups in PD and CAL (all P>0.05), the b-FGF level of the experimental group [(35.28±5.41) pg/30 s] was higher than that of the control group [(33.45±2.37) pg/30 s] ( P<0.05), and the serum hs-CRP level of the experimental group [(3.23±1.73) mg/L] was significantly lower than that of the control group [(5.79±0.63) mg/L] ( P<0.05). Conclusions:Low-level laser therapy can effectively reduce the SBI of severe periodontitis patients in the short term, but it has little significance for reducing PD and improving CAL. At the same time, low-level laser therapy can reduce the serum hs-CRP level of patients and increase the level of b-FGF in gingival sulcus fluid, which is beneficial to the control of severe periodontal inflammation.

Effect of low le vel laser treatment combined with periodontal initial therapy on IL-8, b-FGF and IL-1βcontent in gingival crevicular fluid on peri-implantitis / 国际生物医学工程杂志

Objective To investigate the effect of low level laser treatment combined with periodontal therapy on interleukin-8 (IL-8), basic fibrobast growth factor (b-FGF) and interleukin-1β (IL-1β) level in gingival crevicular fluid on peri-implantitis , so as to evaluate the curative effect . Methods A total of 38 patients (50 implants) with early peri-implantitis were randomly divided into experimental group and control group, with 19 patients (25 implants) in each group. The patients in the experimental group were underwent level laser treatment combined with periodontal initial, and in the control group were only treated with periodontal initial. The implant gingival crevicular fluid was collected before the treatment (baseline), and 4 weeks and 12 weeks after the treatment. The levels of IL-8, b-FGF and IL-1βwere determined by enzyme-linked immunosorbent assay (ELISA). The SPSS19.0 software was used for the statistically analysis. Results The levels of IL-8, b-FGF and IL-1β of the experimental group and the control group were both improved at 4 weeks and 12 weeks after treatment comparing with that at the baseline, and the differences were statistically significant (all P<0.01). The differences in IL-8 and b-FGF levels in the gingival crevicular fluid were statistically significant at different time points for each group (all P<0.01). For the two groups, the differences in IL-1βwere not statistically significant at 4 weeks and 12 weeks after the treatment (all P>0.05). The levels of IL-8 and IL-1βof the experimental group were lower than those of the control group at 4 weeks after treatment, and the differences were statistically significant (all P<0.05). The level of b-FGF of the experimental group was higher than that of the control group at 12 weeks after the treatment, and the difference was statistically significant (P<0.05). Conclusions Low-energy laser can affect the level of cytokines in the gingival crevicular fluid on peri-implantitis. The combination of low level laser treatment and periodontal initial therapy is effective for treating peri-implantitis.

Effect of low energy laser therapy on peri-implantitis in clinical patients / 国际生物医学工程杂志

Objective To study the effectiveness of basic therapy combined with low energy laser irradiation therapy to the patients with peri-implantitis, and to clarify the effect of laser irradiation in the treatment process. Methods A total of 38 patients (50 implants) who were diagnosed with early peri-implantitis from September 2017 to March 2018 in the Department of Stomatology, Tianjin First Central Hospital were randomly divided into the laser treatment group (n=25) and the conventional treatment group (n=25). The control group received basic treatment, and the laser treatment group received basic treatment combined with low energy laser irradiation (808 nm GaAlAs semiconductor continuous laser, average output power 80 mw, energy density 4 J/cm2, facula area 0.28 cm2, 15 seconds irradiation for each implant bag). The GIgingival index (GI), plaque index (PLI), sulcus bleeding index (SBI) and probe depth (PD) were examined before and 2 weeks and 8 weeks after the treatment. Results At 2 weeks and 8 weeks after the treatments, the indexes of the two groups were both improved compared with the baseline, and the differences were statistically significant (all P<0.05). Compared with the control group, the GI was significantly decreased (P=0.018) at 2 weeks after the treatment, and the GI, PD, and SBI were significantly decreased at 8 weeks after the treatment (P<0.001, P=0.018, and P<0.001, respectively). Conclusions At an energy density of 4 J/cm2, low energy laser irradiation at a wavelength of 808 nm can accelerate tissue healing of peri-implant inflammation after basic treatments.

Clinical observation of preservation of inclined fractured permanent molars by transverse fixation with screw post / 天津医药

Objective To explore the effect of preservation of inclined fractured permanent molars by transverse fixation with screw post,and provide a practical method for reserving the inclined fractured permanent molars.Methods Clinical data of 50 patients with inclined fractured permanent molars by transverse fixation with screw post in the Department of Stomatology of Tianjin First Central Hospital between January 2010 to December 2011 were reviewed retrospectively.After fixing the crown with steel-wire binding and root canal treatment,the molars were drilled a hole buccolingually and placed screw post.The flowable light-curing composite resin was used to seal the crack and bond screw post to prevent the relative movement.All treated molars were restored with full crown.All patients were followed up for 10-60 months.The therapeutic effects were observed.Results After 5 year-follow-up,41 cases were cured completely,6 cases were cured clinically,and 3 cases failed treatment.Conclusion The transverse fixation with screw post and bonding technique can preserve the inclined fractured molars to gain chewing function and avoid or reduce the extraction of affected teeth,which provides a better restoration effect.

Exploring application of WeChat for gestational diabetes mellitus management / 中国实用护理杂志

Objective To researching the effect of gestational diabetes mellitus (GDM) prevention and control management by WeChat consultation on the Internet platform. Methods There were 586 pregnant women who performed prenatal care regularly and accepted glucose tolerance test for GDM at 24 to 28 weeks in our hospital during 2015 and 2016 as research objects. Determination of the observation group:in 2016, the 308 people who match the condition of the study during annual prenatal examination in line, in addition to routine guidance outside the clinic and joined the division to establish a dedicated WeChat group for a comprehensive health management consulting. Determination of the control group:in 2015, the 278 people who match the condition of the study during annual prenatal examination in line were treated as control groups. The two groups of observation indexes included the fasting blood glucose and two-hour postprandial blood glucose control in GDM pregnant women, the weight growth rate of the whole pregnancy, cesarean section rate, birth rate of newborns, neonatal referral rate and so on. Results The rate of fasting blood glucose and two-hour postprandial blood glucose control in the observation group was significantly better than that in the control group (t=2.01-3.11, all P<0.05). According to the pre-pregnancy physical index, the weight growth of the observation group was significantly better than the control group (t=2.12-3.76, all P<0.05).The cesarean section rate, birth rate and neonatal referral rate were 21.10％(65/308), 4.87％(15/308), 7.14％(22/308)in the observation group , and 28.78％(80/278), 10.07％(28/278), 12.23％(34/278)in the control group, there were significant differences (U = 2.10-2.42, all P<0.05). Conclusions Gestational diabetes prevention and control management in the Internet platform for WeChat consultation application effect is remarkable, which can effectively improve the maternal and child outcomes. In the protection of maternal and child health is of great significance.

Effect of low 1evel laser treatment on metabolic control and high-sensitivity C-reactive protein of type 2 diabetes mellitus combined chronic peridontitis patients / 国际生物医学工程杂志

Objective To investigate the effect of low 1evel laser treatment (LLLT) on periodontal clinical index,high-sensitivity C-reactive protein (hs-CRP) and metabolic control in patients of type 2 diabetes mellitus combined chronic peridontitis.Methods Nighty patients with type 2 diabetes combined chronic peridontitis were divided into three groups:initial periodontal therapy (group A),initial therapy combined with LLLT (group B) and control group (group C).The periodontal clinical parameters including periodontal probing depth (PD),clinical attachment loss (CAL),sulcus bleeding index (SBI),hs-CRP,HbA1c and fasting blood glucose (FBG) were tested before and at three months after therapy.Results The periodontal clinical parameters (PD,CAL,SBI) and hsCRP in group A and B improved significantly compared with that of group C after therapy (P＜0.05),with group B had more significant difference (P＜0.01).For the levels of HbA1c and FPG,group A and B showed downtrend,and more obvious outcome was detected in group B (P＜0.05).Conclusions LLLT can effectively improve periodontal status,hs-CRP levels and metabolic control of patients with type 2 diabetes combined with chronic peridontitis,and thus to prevent the occurrence of diabetes complications.

Study on the effect of low level laser in the treatment of periodontal disease / 国际生物医学工程杂志

Objective The purpose of this study was to explore the efficacy of low level laser treatment (LLLT) combined with initial periodontal therapy in treating chronic periodontits.Methods Eighteen patients with chronic periodontitis were randomly treated in a spit-mouth design with combination of laser (808 nm,80 mW) treatment with scaling and root planing (experimental) or with scaling and root planing alone (contol) after OHI and supragingival cleaning.Clinical parameters of sulcus bleeding index (SBI),periodontal probing depth (PD) and clinical attachment loss (CAL) of both sides were recorded and gingival crevicular fluid samples were collected at baseline and 1,4,12 weeks after the treatment.GCF samples were analyzed for b-FGF,IL-1β,IL-8 level using ELISA assay.Results For the change of SBI and PD,only SBI in the experimental group experienced significant reduction (P＜0.05) at 1 week compared with baseline (P＜0.05),and the reduction in both groups were statistically significant (P＜0.05) at 4 weeks.The same situation occurred while both experimental groups made more significant difference (P＜0.01) at 12 weeks.The reduction of SBI was obvious in both groups when the PD was more than 6 mm.For CAL,the experimental group showed signs of improvement (P＜0.05) at 4 weeks; both groups indicated improvement (P＜0.05),while the experimental was more significant (P＜0.01) at 12 weeks.For the levels of b-FGF,it showed steady rise after treatment in experimental group,while it went up to the baseline after falling in control group.At 12 weeks,there were statistical significance of differences between two groups (P＜0.05).The changes of IL-1β and IL-8,in the experimental group showed better results than that in control group,while the difference between two groups was not significant (P＞0.05).Conclusion LLLI can accelerate the healing of periodontal tissue by reducing the SBI,decreasing the PD,improving the CAL and increasing levels of b-FGF in the GCF of the patients.