RESUMO
Activation of inflammatory responses regulates the transmission of pain pathways through an integrated network in the peripheral and central nervous systems. The immunopotentiator thymosin alpha-1 (Tα1) has recently been reported to have anti-inflammatory and neuroprotective functions in rodents. However, how Tα1 affects inflammatory pain remains unclear. In the present study, intraperitoneal injection of Tα1 attenuated complete Freund's adjuvant (CFA)-induced pain hypersensitivity, and decreased the up-regulation of pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6) in inflamed skin and the spinal cord. We found that CFA-induced peripheral inflammation evoked strong microglial activation, but the effect was reversed by Tα1. Notably, Tα1 reversed the CFA-induced up-regulation of vesicular glutamate transporter (VGLUT) and down-regulated the vesicular γ-aminobutyric acid transporter (VGAT) in the spinal cord. Taken together, these results suggest that Tα1 plays a therapeutic role in inflammatory pain and in the modulation of microglia-induced pro-inflammatory cytokine production in addition to mediation of VGLUT and VGAT expression in the spinal cord.
RESUMO
Objective To study the relationship between the cerebrospinal fluid (CSF) volume and intracranial measurement line in normal Chinese by useing CT data and computer technology.Methods By CT scan,to measure CSF volume and third ventricle maximum width,suprasellar cistern minimus width,lateral ventricle width in hypothalamic layer,caudate nucleus pitch and other index by age and gender in 178 normal adults.Results There were significant difference between male and female CSF volume in the groups of 14-20 years(23 cases),31-40 years(33 cases),41-50 years(32 cases) and ＞ 60 years (13 cases)(P＜ 0.05 or ＜ 0.01).The CSF volume between male and female had significant difference (t =3.302,P =0.001).The CSF volume and third ventricle maximum width had significant correlation (r =0.685).Conclusion The third ventricle maximum width is the most closely with CSF volume.
RESUMO
We investigated the transesterification of crude cottonseed oil with methyl acetate to biodiesel, by using Lipozyme TL IM and Novozym 435 as catalysts. Results showed that the biodiesel yield significantly increased with the addition of methanol into the reaction system, and the highest biodiesel yield of 91.83% was achieved with the optimum conditions as follows: n-hexane as solvent, molar ratio of methyl acetate to oil 9:1, 3% methanol based on the oil mass to inhibit the creation of acetic acid, 10% Lipozyme TL IM and 5% Novozym 435 as catalyst based on the oil mass, reaction temperature 55 degrees C and reaction time 8 h. Additionally, we explored the kinetics of lipase-catalyzed crude cottonseed oil to biodiesel, and proposed a kinetic model.