Real-time detection of mast cell degranulation in anaphylactoid reaction / 中国中药杂志

<p><b>OBJECTIVE</b>To establish a new, real time, dynamic and direct optical detection method for mast cell degranulation caused by anaphylactoid reaction.</p><p><b>METHOD</b>A CD63-GFP plasmid was constructed and introduced steadily into rat basophilic leukemia (RBL-2H3) cells. The movements of CD63-GFP, which was located on both the granule membranes and the plasma membranes of RBL cells stimulated by Compound 48/80, were studied by confocal laser scanning microscope (CLSM) and total internal reflection fluorescence microscope (TIRFM) both inside and on the surface of living RBL-2H3 cells.</p><p><b>RESULT</b>Before antigen stimulation, most granules with CD63-GFP hardly moved in RBL cells. However, after antigen stimulation, the granules moved dramatically. They reached the plasma membranes in a few minutes and fused with them instantaneously. The velocity of the granule movement toward the plasma membranes on antigen stimulation was calculated to be 0.05 micron x s(-1).</p><p><b>CONCLUSION</b>Analysis of the movement of each granule provided a new insight into the elementary process of degranulation. The method is rapid, sensitive and reliable, which could be used as a new detection method for anaphylactoid reaction in vitro.</p>

A new evaluation method for tumor cell migration process / 中国药理学通报

Aim To establish a new,multi-parameter,real time and qualitative cell migration evaluation method.Methods Lung cancer cell line A549 was cultured on the glass bottom dish.After treated with different dosages of berberine or Rg3 for 24 hours,several scratching lines at the same dimension were made and observed by Living Cell Working Station.8 observation areas were selected randomly and imaged continuously for 24 hours.Transferred Area(TA),Transferred Distance(TD),Single Cell Transferred Speed(SCTS)and the Cell Division Number among defined area(CDN)were analyzed after getting sequence images.Results The focus stage and the incubation system were sufficient to keep cell proliferation and made it possible for long term observation.Berberine at 25 μmol·L~(-1) and 12.5 μmol·L~(-1) could inhibit the migration of A549(P<0.01).The analysis results of TA,TD,SCTS and CDN were basically coincident.Rg3 at 0.1 mmol·L~(-1) could inhibit SCTS and promote CDN in 6,12 and 24 h(P<0.01),while decrease both TA and TD in 24 hs.Conclusion The method is sensitive,rapid and simple to be applied in the research of tumor metastasis,wound healing and inflammatory response with real time,in-situ and multi-parameters.

Laser scanning confocal microscope techonology and its application in biomedical field / 国际中医中药杂志

Here we reviewed the applications of laser scanning confocal microscope in biomedical field. The reformation and improvement based on this technique were also summarized. After analyzing the defects, we highlighted its potential applications in the future.

Changes of Electrolyte in the Children Suffered from Pneumonia / 中国医师杂志

Objective To study the character and clinical significance of electrolyte in the children suffered from different degree pneumonia on different periods. Methods The levels of sodium, calcium, magnesium, potassium, chlorine and phosphorus in the serum of children suffered from mild pneumonia group, severe pneumonia group and control group were tested respectively. The levels of sodium, calcium, magnesium in the serum of children suffered from pneumonia during their acute period and convalescence were tested respectively. The levels of potassium in the serum of children suffered from pneumonia caused by various pathogens were detected too. Those results were analysed. Results The levels of sodium, calcium, magnesium in the serum of children in mild pneumonia group were obviously higher than those in severe pneumonia group(P

CHANGES OF GASTRIC AND INTESTINAL MUCOUS MEMBRANE IN THE RAT MODEL OF "EXPERIMENTAL DEFICIENCY SPLEEN SYNDROME"——A SCANNING ELECTRON MICROSCOPIC STUDY / 解剖学报

The experiment is based on the Traditional Chinese Medicine Theory "Spleen and stomach can be injured or caused functional deficiency by fatigue, starvation or overeating".Wistar rats were used in the experiment and divided into two groups according to their weight. Fatigue, starvation or overeating models were produced by various methods. The results obtained by SEM showed that the gastric and intestinal mucosa were normal in the control group and erosion of mucosa appeared in the experimental group. The erosion was more severe in the heavy weight rats than that of the light weight rats.