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Objective@#To analyze the changes in coronary arterial microcirculation index and its possible influencing factors in elderly patients with hypertension.@*Methods@#In the retrospective case-control study, 36 elderly patients with hypertension in our hospital from May 2016 to April 2017 and meeting inclusion criteria were recruited as the hypertension group, and 36 matched non-hypertensive elderly patients during the same time were considered as the non-hypertensive group.Patients in the two groups underwent coronary angiography and percutaneous coronary function tests for the coronary heart disease symptoms such as chest pain and chest tightness.The changes of coronary microcirculation index were observed, and its possible influencing factors were analyzed in combination with other test results.@*Results@#The low-density lipoprotein level was lower, and plasma viscosity, erythrocyte sedimentation rate, serum high-sensitivity C-reactive protein and 24-h urinary protein were higher in the hypertension group than in the non-hypertensive group(P<0.05). The parameters were higher in the hypertension group versus in control group as follows: Left ventricular ejection fractions(LVEF), [(74.82±6.71 )% vs.(71.56±4.93 )%], Left ventricular mass[(189.72±33.40)g vs.(174.38±29.11)g], Left ventricular mass index[(101.70±16.25)g/cm2 vs.(89.68±10.46)g/cm2](t=2.077, 3.731 and 4.676, P<0.05 or P<0.01), and the proportion of patients with decreased LVEF(52.8%, 19 cases vs.27.8% 10 cases, χ2=4.676). Multivariate logistic regression analysis showed that low-density lipoprotein(OR=2.887, 95%CI: 1.754~8.193, P=0.035), plasma viscosity(OR=4.075, 95%CI: 1.629~7.386, P=0.001), erythrocyte sedimentation rate(OR=3.534, 95%CI: 1.293~6.730, P=0.001), serum high-sensitivity C-reactive protein(OR=2.821, 95%CI: 1.546~7.386, P=0.001), 24-h urinary protein(OR=3.712, 95%CI: 1.683~7.954, P=0.026), left ventricular mass index(OR=3.254, 95%CI: 1.286~6.735, P=0.004)and reduced left ventricular diastolic function(OR=5.069, 95%CI: 1.386~7.254, P=0.007)were the risk factors for abnormal changes in coronary microcirculation resistance index.@*Conclusions@#Elderly hypertensive patients have abnormal coronary microcirculation resistance index, and the intervention should be strengthened from the aspects of blood lipid, plasma viscosity, , inflammation, renal function and cardiac function, so as to improve coronary function and reduce adverse cardiovascular events.
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Objective To analyze the changes in coronary arterial microcirculation index and its possible influencing factors in elderly patients with hypertension.Methods In the retrospective case-control study,36 elderly patients with hypertension in our hospital from May 2016 to April 2017 and meeting inclusion criteria were recruited as the hypertension group,and 36 matched nonhypertensive elderly patients during the same time were considered as the non-hypertensive group.Patients in the two groups underwent coronary angiography and percutaneous coronary function tests for the coronary heart disease symptoms such as chest pain and chest tightness.The changes of coronary microcirculation index were observed,and its possible influencing factors were analyzed in combination with other test results.Results The low-density lipoprotein level was lower,and plasma viscosity,erythrocyte sedimentation rate,serum high-sensitivity C-reactive protein and 24-h urinary protein were higher in the hypertension group than in the non-hypertensive group(P＜0.05).The parameters were higher in the hypertension group versus in control group as follows:Left ventricular ejection fractions (LVEF),[(74.82 ±-6.71) ％ vs.(71.56 ±-4.93) ％],Left ventricular mass[(189.72 ± 33.40) g vs.(174.38 ± 29.11) g],Left ventricular mass index [(101.70 ± 16.25)g/cm2 vs.(89.68 ± 10.46) g/cm2] (t =2.077,3.731 and 4.676,P ＜0.05 or P ＜0.01),and the proportion of patients with decreased LVEF (52.8％,19 cases vs.27.8％ 10 cases,x2 =4.676).Multivariate logistic regression analysis showed that low-density lipoprotein(OR =2.887,95 ％ CI:1.754～ 8.193,P =0.035),plasma viscosity(OR =4.075,95％ CI:1.629 ～ 7.386,P =0.001),erythrocyte sedimentation rate (OR =3.534,95％ CI:1.293 ～ 6.730,P =0.001),serum highsensitivity C-reactive protein (OR =2.821,95 ％ CI:1.546 ～ 7.386,P =0.001),24-h urinary protein (OR=3.712,95％CI:1.683～7.954,P =0.026),left ventricular mass index(OR =3.254,95％CI:1.286 ～ 6.735,P =0.004)and reduced left ventricular diastolic function(OR =5.069,95 ％ CI:1.386 ～7.254,P=0.007)were the risk factors for abnormal changes in coronary microcirculation resistance index.Conclusions Elderly hypertensive patients have abnormal coronary microcirculation resistance index,and the intervention should be strengthened from the aspects of blood lipid,plasma viscosity,,inflammation,renal function and cardiac function,so as to improve coronary function and reduce adverse cardiovascular events.
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Objective To observe the damage of various organs of rats with exertional heatstroke (EHS), and to investigate the protective effect of oral rehydration salts Ⅲ (ORSⅢ) on multi-organ function in rats with EHS. Methods Fifty-one male Sprague-Dawley (SD) rats were randomly divided into four groups by random digit table: normal control group (n = 13), EHS group (n = 13), EHS+water group (n = 12), and EHS+ORSⅢ group (n = 13). All rats in the EHS groups received adaptive training for 7 days before the experiment. On the 8th day, the rats of EHS+water and EHS+ORSⅢ groups were orally given 20 mL/kg water or ORSⅢ 30 minutes before the experiment. No pretreatment was performed in the EHS group. EHS model was reproduced by forcing rats to run under hot environment. The rats which refused to exercise and which core temperature > 40.5 ℃ were considered as the onset of EHS. The rats in the normal control group were exposed to room temperature (25±2) ℃ and humidity (50±5)% without any treatment. Six hours later, blood of inferior vena cava was collected, and the levels of serum MB isoenzyme of creatine kinase (CK-MB), lactate dehydrogenase (LDH), alanine transaminase (ALT), aspartate transaminase (AST), serum creatinine (SCr), blood urea nitrogen (BUN), serum potassium, serum sodium and serum chloride were determined by automatic chemical analyzer. Serum intestinal fatty acid-binding protein (I-FABP) was determined by enzyme linked immunosorbent assay (ELISA). Results The levels of LDH, ALT, AST, BUN, serum sodium and serum chloride in the EHS group were significantly higher than those in the normal control group [LDH (U/L): 1 220±427 vs. 837±485, ALT (U/L): 138 (97, 164) vs. 37 (33, 42), AST (U/L): 409 (380, 566) vs. 86 (78, 104), BUN (mmol/L): 11.7 (9.6, 13.2) vs. 5.9 (5.5, 6.1), serum sodium (mmol/L): 148.0 (143.5, 154.5) vs. 139.0 (138.0, 140.5), serum chloride (mmol/L): 100.9±2.3 vs. 97.3±1.4, all P < 0.05], but no significant difference in CK-MB, SCr or serum potassium could be found [CK-MB (U/L): 1 280±373 vs. 1 379±480, SCr (μmol/L): 38.2±7.5 vs. 35.5±6.3, serum potassium (mmol/L): 5.5 (4.4, 6.2) vs. 4.7 (4.4, 4.9), all P > 0.05]. In the EHS+ORSⅢ group, only serum potassium level was significantly lower than that in the EHS group [mmol/L: 4.0 (3.7, 4.4) vs. 5.5 (4.4, 6.2), P < 0.01], while no significant difference in other parameters was found between the EHS+ORSⅢ group and the EHS group as well as the EHS+water group. Serum I-FABP level in the EHS group was significantly higher than that in the normal control group [μg/L: 36.90 (29.10, 45.00) vs. 11.39 (0.31, 20.80), P < 0.01]. Serum I-FABP level in the EHS+water and EHS+ORSⅢ groups were notably lower than that in the EHS group [μg/L:24.19 (20.00, 28.36), 0.31 (0.31, 5.58) vs. 36.90 (29.10, 45.00), both P < 0.01], additionally, I-FABP level was much lower in the EHS+ORSⅢ group (P < 0.01). Conclusions EHS could lead to liver, intestinal barrier dysfunction and electrolyte disturbance. Pre-treatment of ORSⅢ could alleviate the intestinal dysfunction and electrolyte disorder caused by EHS in rats. It can lower the serum potassium to some extent. However, ORSⅢ failed to protect liver from EHS.
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Objective To summarize surgery preparation and nursery care for free intemational cataract surgery in the Islamic Republic of Mauritania and provide reference for eye care in future international cataract program.Method Prepare staff and sufficient material before surgery appropriately,pay sufficient attention to infection prevention during surgery,disseminate antibiotic eye-drop to the patient and arrange for regular follow-up after operation.Result Toally 200 cataracted surgeries were performed without severe eye and systemic complications,vision improved for all the patients.Conclusion It is critical to make sufficient staff planning and materials preparation for international cataract surgery program,meanwhile,careful nurse eye care is indispensable for the success of surgeries.
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Objective To observe the epithelial mesenchymal transition (EMT) of podocyte induced by high glucose,and to explore the potential protective mechanism of ursolic acid (UA).Methods The podocytes cultured in vitro were divided into four groups:normal group (glucose 5.5 mmol/L),mannitol group (glucose 5.5 mmol/L+mannitol 19.5 mmol/L),high glucose group (glucose 25 mmol/L) and UA group (glucose 25 mmol/L + UA 5 μmol/L).Podocyte morphology changes were observed by inverted phase contract microscope.The expression of zonula occludens-1 (ZO-1) and α-smooth muscle actin (α-SMA) were detected by immunofluorescence.The expressions of β-catenin and glycogen synthesis kinase-3β (GSK3β) were detected by Western blotting.The expressions of Wnt1,Wnt3a,Wnt5a,Wnt5b and GSK3β were detected by real-time PCR.Results Podocytes showed irregular arborization shape in normal glucose and transited to longer cobblestone-like shape as mesenchyme cell by high glucose culture.Compared with normal group,the expression of ZO-1 protein was down-regulated and the expression of α-SMA was up-regulated by high glucose culture (P ＜ 0.05).The expression of Wnt5a mRNA was down-regulated;β-catenin mRNA and protein were up-regulated (P ＜ 0.05);and GSK3β protein was down-regulated by high glucose culture (P ＜ 0.05).Compared with high glucose group,ursolic acid inhibited podocyte EMT,up-regulated the expression of ZO-1 protein,Wnt5a mRNA,GSK3β (P ＜ 0.05),and down-regulated the expressions of α-SMA protein,β-catenin mRNA and protein (P ＜ 0.05).Conclusion Ursolic acid attenuates high glucose induced epithelial mesenchymal transition of podocyte by inhibiting Wnt/β-catenin signaling pathway.
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Objective To explore the influence of the protection motivation theory (PMT) on the self-nursing ability of high-risk diabetic foot (DF) patients. Methods The outpatients in our hospital were selected with high-risk DF between January 2013 and May 2014, randomly divided into the control group and PMT group, 52 cases in each group. Guided under the protection motivation theory, PMT group received a six-month health education and management; and the control group accepted conventional health education of diabetes. Before and after the intervention, some observation indexes of the two groups respectively were evaluated including the ability of diabetic foot self-nursing, foot condition, fasting blood sugar, 2 h postprandial blood glucose. Result After six months, the scores of the self-care ability of diabetic foot and foot condition from the patients of PMT group were higher than that of PMT group before the intervention and that of control group after intervention (P<0.05). Conclusion PMT can help patients with high-risk DF enhance their foot self-care ability, improve their foot condition, control their blood sugar, and prevent the DF onset.
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ObjectiveTo study the diagnostic value of sonographic “Leopard pattern” sign in breast hyperplasia.MethodsTwo hundred and twenty-three female patients in Beijing Friendship Hospital from October 2012 to April 2013 were choosen. All the cases were diagnosed as mammary gland hyperplasia clinically, and their breast ultrasound images show the breast imaging-reporting and data system (BI-RADS) grad was 0 or 1. These patients were divided into 3 groups according to their age, Group A: 20 to 35 age (36 cases), Group B: 36 to 50 age (102 cases), and Group C: order than 50 year-old (85 cases). The pathological diagnosis were obtained by ultrasound guided breast biopsy. With pathological diagnosis as the gold standard and “Leopard sign” as ultrasound diagnostic criteria, the sensitivity, specificity, accuracy, positive predictive value and negative predictive value were calculated. The consistency between the ultrasonic and the pathological diagnosis were analyzed by Kappa statistical test.ResultThe diagnostic consistency test of the total sample of 223 cases: in terms of the ultrasonic Leopard sign” for the diagnosis of breast hyperplasia, the sensitivity was 69% (59/85),the specificity was 46% (64/138), the accuracy was 55% (123/223), the positive predictive value was 44% (59/133), the negative predictive value was 71% (64/90), the consistency was poor (Kappa=0.14,P<0.05). Group A:the sensitivity was 90% (18/20), the specificity was 50% (8/16),the accuracy was 72% (26/36), the positive predictive value was 69% (18/26), the negative predictive value was 80% (8/10), the consistency was good (Kappa=0.42, P<0.05);Group B:the sensitivity was 70% (33/47), the specificity was 49% (27/55),the accuracy was 59% (60/102), the positive predictive value was 54% (33/61), the negative predictive value was 66% (27/41), the consistency was poor (Kappa=0.19,P<0.05); Group C: the sensitivity was 44% (8/18), the specificity was 43% (29/67), the accuracy was 44% (37/85), the positive predictive value was 17% (8/46), the negative predictive value was 74% (29/39), the consistency was poor (Kappa=0.08,P<0.05).Conclusion“Leopard pattern” sign is not suitable as an independent standard in diagnosing breast hyperplasia disease.
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Craniofacial malformation was related to the shape and structure of the teeth, jaws and face. Assessment of craniofacial complex in three-dimensional (3D) has long been a focus for many researchers. At present, 3D imaging techniques could reconstruct the craniofacial soft tissue or hard tissue successfully. However, they couldn't reflect the comprehensive structure of craniofacial complex. For the study of the craniofacial complex, combination of 3D imaging techniques provides a promising way toward optimum 3D imaging. The purpose of this study was to reconstruct the 3D images of craniofacial hard and soft tissue based on cephalograms and laser scanning, to found the 3D measurement and orthognathic simulation and forecast system, which may provide a new method for analyzing of craniofacial malformation, and for the quantity control and evaluation of treatment results in the orthodontic treatment, orthopedics and plastic operation.
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Humanos , Face , Processamento de Imagem Assistida por Computador , Imageamento Tridimensional , Lasers , Cirurgia Ortognática , Tomografia Computadorizada por Raios XRESUMO
Biometric recognition methods for human identification have received researchers' attention. Seeing that the prominent characteristics of skull biometrics are not affected by facial expression and illumination, we present a new approach compounded of several features. Firstly, comparing the crania included angle; secondly, sampling the edge as the skull feature and analyzing the skull feature in multi-resolution; lastly, calculating the crania cavity area and regularizing the recognition rules. This method supports human identification in cases not only ante mortem but also postmortem.
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Humanos , Algoritmos , Cefalometria , Métodos , Interpretação de Imagem Assistida por Computador , Processamento de Imagem Assistida por Computador , Métodos , Radiografia , Crânio , Diagnóstico por ImagemRESUMO
Skull recognition is a new method of biometrics recognition. A skull recognition algorithm is presented in this paper by Quadratic rational Bezier curve fitting which accurately describes the feature of skull edge; the experiment results based on skull x-ray image show the correctness of this method.
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Humanos , Algoritmos , Artefatos , Reconhecimento Automatizado de Padrão , Métodos , Intensificação de Imagem Radiográfica , Métodos , Interpretação de Imagem Radiográfica Assistida por Computador , Métodos , Crânio , Diagnóstico por ImagemRESUMO
<p><b>OBJECTIVE</b>To study the influence of heat-induced epitope retrieval (HIER) on endogenous avidin-binding activity (EABA) and to establish an effective way to block EABA in immunohistochemistry.</p><p><b>METHODS</b>Systematically screening EABA in 164 (679 samples) formalin-fixed and paraffin-embedded human tissues including 76 (102 samples) normal tissues and 88 (577 samples) tumor tissues as well as 4 (80 samples) formalin-fixed and paraffin-embedded rat normal tissues using tissue array (tissue chip), HIER, immunohistochemistry and egg white solution blocking. In addition, EABA was also examined in 9 (15 samples) human frozen tissues.</p><p><b>RESULTS</b>(1) EABA was detected in frozen tissues. (2) No staining for EABA was seen in formalin-fixed and paraffin-embedded tissues. (3) EABA was revealed after the tissues treated with microwave HIER. (4) The density of signal for EABA was variable from tissue to tissue and cell to cell. (5) The signals of EABA expressed in scatter or diffuse in tissues and in granular form in cytoplasm. (6) EABA was found in a wide range of epithelial tissues, especially in gland epithelia of normal and tumor tissues. These included kidney, adrenal cortex, liver, C cells of thyroid gland, oxyphil cells of parathyroid, fundal gland of stomach, sebaceous gland of skin, duct of salivary; oncocytoma and papillary adenocarcinoma of kidney and thyroid gland, adenolymphoma of parotid, carcinoma of liver cell, adenocarcinoma of stomach, colon, prostate, gall bladder and endometrium, and so on. (7) EABA was easier revealed by higher pH value buffer (EGTA pH 9.0) than that with lower pH value (EDTA pH 8.0 and citrate pH 6.0). (8) The revealed EABA could be effectively blocked using 20% egg white solution.</p><p><b>CONCLUSIONS</b>HIER could unmask EABA in formalin-fixed and paraffin-embedded tissues. The unmasked EABA present in a wide range of human normal and tumor tissues as well as in rat normal tissues. The EABA could influence routine immunohistochemistry staining when using (strept)avidin-horseradish peroxidase detective system. The egg white solution could effectively block EABA and eliminate the influence of EABA on immunohistochemistry.</p>