A case of podocyte infolding glomerulopathy / 中华肾脏病杂志

Podocyte infolding glomerulopathy (PIG) is a pathologic type of podocyte glomerulopathy reported recently. The characteristic is that the ultrastructure related to podocytes, such as microspheres and microtubules, are folded into the glomerular basement membrane (GBM) under electron microscope. At present, there are few reports about this disease at home and abroad, and most of them are concentrated in Japan. The clinical characteristics and pathogenesis of PIG are still unclear. In this paper, we report a case of clinical manifestations of nephrotic syndrome, renal biopsy indicated PIG, after the treatment of glucocorticoid, hydroxychloroquine and tacrolimus, the patient's clinical symptoms were relieved and urinary protein decreased.

Effective dose of esketamine for prevention on propofol injection pain in painless abortion / 中国临床药理学与治疗学

AIM: To investigate the effective dose of esketamine for prevention on propofol injection pain in painless abortion. METHODS: From November 2021 to December 2021, thirty patients undergoing painless abortion, aged 20 to 40 years old, ASA physical status or Ⅱ, BMI 19-26 kg/m

Syphilis infection status among voluntary blood donors in Nanyang area and preventing strategy of blood transfusion transmitted syphilis / 中国输血杂志

【Objective】 To analyze the infection status of syphilis among voluntary blood donors in Nanyang area, aimed to provide references for the recruitment of blood donors and ensure blood safety. 【Methods】 Enzyme-linked immunosorbend test(ELISA method) was used to screen the blood samples of 45 6981 voluntary blood donors from 2013 to December 2020, and the reactive blood samples of TP antibody were confirmed by treponema pallidum particle agglutinaation(TPPA method), the syphilis infection status in different population was compared and analyzed. 【Results】 Among 456 981 blood samples, 1 088 were reactive for anti-TP by ELISA method, and 714 were positive for syphilis(714/456 981, 0.15%); the total positive rate and positive rate of male and female in voluntary blood donors decreased year by year; female donors(347/180 987, 0.192%), first-time donors(613/324 188, 0.189%), whole blood donors(708/433 432, 0.163%) and married donors(668/399 899, 0.167%) had a significantly higher syphilis seroprevalence than male donors(367/275 994, 0.133%), repeated donors(101/132 793, 0.076%), apheresis platelet donors(6/23 549, 0.025%) and unmarried donors(46/57 082, 0.080%), respectively, and the differences between two groups were statistically significant (P<0.05); the positive rate in donors with junior high school and below was significantly higher than other groups; the positive rate in donors of 35~44 years old was higher than other age groups, with statistically significant difference(P<0.05); syphilis seroprevalence among different occupational groups was statistically significant (P<0.05); Among 714 donors, co-infection with other viruses were noted in 40 cases(40/714, 5.60%), and only TP infection in 674 cases(674/714, 94.40%). 【Conclusion】 The TP seroprevalence among voluntary blood donors in Nanyang area showed a downward trend from 2013 to 2020, the majority of TP seropositive population are first-time donors, married donors, whole blood donors, donors with lower academic qualifications, and farmers/individual donors. Strengthening the consultation towards high-risk blood donors before blood donation, and recruitment of blood donors from low-risk groups can reduce / avoid the transmission of syphilis through blood transfusion and ensure the safety of blood transfusion.

Efficacy of ultrasound-prepositioned four-point method for right internal jugular vein catheterization in parturients at high risk of bleeding / 中华麻醉学杂志

Objective To evaluate the efficacy of ultrasound-prepositioned four-point method for right internal jugular vein ( IJV) catheterization in the parturients at high risk of bleeding. Methods Eighty American Society of Anesthesiologists physical status Ⅰ or Ⅱparturients diagnosed as having perni-cious placenta previa, aged 25-38 yr, weighing 60-90 kg, scheduled for elective cesarean section under general anesthesia, were divided into 2 groups ( n=40 each) using a random number table method: real-time ultrasonic guidance group ( group UG ) and ultrasound-prepositioned four-point method group ( group UF) . In group UG, the right IJV catheterization was performed under real-time ultrasound guidance: mov-ing the ultrasonic probe to make the mid-line of the cross section image of the right IJV overlap with the mid-line of the ultrasonic display screen, and the intersection of the mid-line of the IJV and the horizontal line of the annular cartilage was selected as the puncture point. In group UF, the right IJV catheterization was per-formed by using ultrasound-prepositioned four-point method as follows: moving the ultrasonic probe to make the midline of the right IJV cross-sectional images overlap with the midline of the ultrasound display screen, and marking the skin where the midpoint of the probe's long-axis was located as point A;transversely mov-ing the probe to the inside so that the tangent line of its inner edge overlapped with the midline of the ultra-sonic display screen, marking the skin where the midpoint of the probe's long-axis was located as point B ( the puncture site);at the proximal cardiac end of the right IJV, making point C at 2 cm from point B, and making point D ( the indicator point, direction of the puncture needle) at 2 cm from point A. After lo-cal infiltration anesthesia was performed in point B, the puncture needle was inserted towards the point D. The success rate of puncture, success rate of catheterization, catheterization operation time and complica-tions such as hematoma, intravascular catheter insertion or hemopneumothorax were recorded. Results The success rate of total catheterization was 100％ in two groups. Compared with group UG, the catheteriza-tion operation time was significantly shortened ( P<0. 01) , and no significant change was found in the suc-cess rate of puncture at first attempt, success rate of catheterization at first attempt or intravascular catheter insertion in group UF ( P>0. 05) . Conclusion Compared with real-time ultrasound guidance, ultrasound-prepositioned four-point method produces better efficacy when used for the right IJV catheterization in the parturients at high risk of bleeding.

Research of biological effect of different dose of satereotactic body radiation therapy on hepatoma Hep G2 ceils / 中国医师杂志

Objective To study the biological effect of different dose of satereotactic body radiation therapy (SBRT) on hepatoma Hep G2 Cells.Methods Hep G2 hepatumor cells vitality of 0,2,4,6,8,and 10 Gy doses of SBRT were determined by methyl thiazolyl tetrazolium (MTT) method.The cell colony formation rate and the cell survival fraction were detected.The cell apoptosis was detected by flow cytometry.The expressions of cyclin D1,Bax,Bcl-2,and caspase-3 protein were detected by Western blot.Results Hep G2 cells vitality was obvious inhibitory effect in different doses of SBRT,increasing with the SBRT dose,the cell vitality inhibition [2 Gy group (4.29 ± 0.63) ％,4 Gy group (19.37 ± 4.54) ％,6 Gy group (41.35 ±4.51)％,8 Gy group (86.34 ±5.68)％,10 Gy group (97.24 ±5.41)％] was increased,compared to 0 Gy group [(2.94 ± 0.52) ％],the difference was statistically significant (all P ＜ 0.01 or P ＜0.05).The cell colony formation rate and survival score in different doses of SBRT were statistically lower than the control group,increasing with the SBRT dose,the cell colony formation rate [4 Gy group (83.15 ± 4.71),6 Gy group (65.23 ± 5.47),8 Gy group (41.28 ± 5.36),10 Gy group (8.14 ± 5.22)] and survival score [2 Gy group (95.49 ±3.21),4 Gy group (84.23 ±3.45),6 Gy group (66.08 ± 3.71),8 Gy group (41.82 ± 3.82),10 Gy group (8.25 ± 3.56)] were decreased,compared to 0 Gy group [(98.72 ± 1.23),(100.00 ±0.00)],the differences were statistically significant (all P ＜0.01 or P ＜ 0.05).The cell apoptosis in different doses of SBRT was increased,increasing with the SBRT dose,the Hep G2 cells apoptosis [4 Gy group (13.67 ±4.62),6 Gy group (28.71 ±5.13),8 Gy group (54.16 ±5.24),10 Gy group (76.31 ± 5.17)] were significantly increased,compared to 0 Gy group (1.35 ±0.46),the difference was statistically significant (all P ＜ 0.01).The expressions of cyclinD1,Bax,Bcl-2,and caspase-3 protein in different doses of SBRT were decreased,increasing with the SBRT dose,the cyclin D1 [6 Gy group (1.94 ± 0.57),8 Gy group (1.38 ± 0.41),10 Gy group (0.52 ± 0.34)],Bax[6 Gy group (2.67 ±0.59),8 Gy group (1.73 ±0.45),10 Gy group (0.63 ±0.37)],Bcl-2 [4 Gy group (1.78 ±0.41),6 Gy group (1.03 ±0.35),8 Gy group (0.76 ±0.28),10 Gy group (0.38 ± 0.21)],Caspase-3 [6 Gy group (0.76 ± 0.31),8 Gy group (0.42 ± 0.24),and 10 Gy group (0.24 ± 0.21)] were obviously decreased,compared to 0 Gy group [(3.41 ± 0.75),(4.36 ± 0.87),(2.57 ± 0.46),(1.67 ± 0.55)],the difference was statistically significant (all P ＜ 0.05).Conclusions Different dose of SBRT can reduce Hep G2 cell vitality,enhance radiation sensitivity,have a dose dependent,maybe related to promote cell apoptosis and inhibit the proliferation of protein expression.

Analysis of the biological effects of dose radiotherapy for primary liver cancer patients with stereotactic body portion / 中国基层医药

Objective To study the biological effects of dose radiotherapy for primary liver cancer patients with stereotactic body portion.Methods 80 patients with primary liver cancer were selected.According to a random number table method, all patients were divided into observation group and control group,40 patients in each group.All patients were treated with stereotactic body radiotherapy treatment, the observation group was treated with high dose, while the control group received low-dose.The radiotherapy effects of different doses were analyzed and compared.Results After treatment, the effective rate of the observation group was 77.5%,which of the control group was 55.0%, ,the difference between the two groups was statistically significant (x2=4.528,P=0.033).After treatment for 6 months,1 year and 2 years, the survival rates of the observation group were 77.5%,75.0%,72.5%, which were significantly higher than 55.5%,52.5%,50.0% of the control group (P<0.05).The incidence rate of adverse reactions of the observation group was 42.5%, which was significantly lower than 72.5% of the control group (x2=7.365,P=0.006).Conclusion Stereotactic body radiotherapy treatment with different therapeutic dose has different treatment efficacy, high-dose SBRT treatment has significantly higher efficacy, survival rate and lower adverse reactions, which is worthy of clinical application.

Efficacy and safety of ropivacaine combined with lidocaine in caudal block for pediatric laparoscopic hernia surgery / 中国生化药物杂志

Objective To evaluate the anesthetic effect and safety of ropivacaine combined with lidocaine caudal block in the treatment of pediatric laparoscopic hernia surgery.Methods A total of 70 cases with pediatric hernia surgery in Ningbo Women and Children’s Hospital from May 2014 to October 2015 in this study were divided into control group and experimental group with 35 cases in each group.The patients in the both groups were given routine examination, preoperative anesthesia induction, adopted 7 scalp injection needles to prick into the hiatus sacralis,patients in control group were treated by 1% lidocaine hydrochloride injection mixed liquid 1 mL/kg;patients in the experimental group were treated by 0.25%lidocaine hydrochloride injection and 0.25% ropivacaine hydrochloric injection mixed liquid 1 mL/kg; pediatric hernia surgery, intraoperative continuous pumped into propofol injection 4-6 mL/kg and maintain the mask to the oxygen.The visual analog pain scored( VAS) , visual satisfaction scored ( VSS) and adverse reactions were compared at the end of operation, after operation 0 h,0.5 h,1 h,2 h,4 h,8 h and 24 h.Results The sensory and motor block effective time in the experimental group were later than the control group, the sensory and motor block duration time were shorter than the control group (P<0.05).VAS score of the experimental group was significant lower than control group after operation 2 h ( P<0.05 ) . After operation, postoperative 0.5 h, postoperative 1 h, the VSS score of the experimental group was significant lower than control group ( P<0.05 ) .Lower limbs numbness discomfort duration time of the experimental group was significant lower than control group after operation ( P<0.05 ) . Postoperative agitation rate of the experimental group 8.57% was significant lower than the control group 34.29% (P<0.05).The incidence of adverse reactions of experimental group 5.71% was significant lower than the control group 25.71% ( P<0.05 ) .Conclusion Ropivacaine combined with lidocaine caudal block in the treatment of pediatric hernia surgery has the remarkable effect, with high safety.

Different doses of propofol infusion on serum lipid metabolism and pulmonary function in patients with cervical cancer surgery / 中国生化药物杂志

Objective To investigate effect of different doses of propofol infusion on serum lipid metabolism and pulmonary function in patients with cervical cancer operation.Methods 120 patients with cervical cancer were randomly divided into A, B, C three groups, 40 cases in each group, were treated with laparoscopic cervical cancer radical operation, the 4 mg/(kg· h), 6 mg/(kg· h), 8 mg/(kg· h), and the pulmonary function and lipid profiles were detected.Results Compared with the B and C groups, the recovery time and the the awake time of the patients in B ,C group was shorter (P<0.05).The recovery time and the the awake time of the patients in A group had not significantly different.Compared with before operation, TG, TC and LDL levels were elevated, HDL decreased in theree groups after the operation(P<0.05);compared with the A and B groups, the levels of TC, LDL, TG and were higher, the level of HDL were lower (P<0.05),there were no significant differences in level of TC, HDL, TG and LDL between group A and group B;there was no significant difference between P(A-a) O2 and RI among three groups before surgery, P(A-a) O2 and RI levels were elevated in the three groups after surgery(P<0.05), compared with the A and B groups, P(A-a)O2 and RI levels in group C were lower(P<0.05), there was no significant difference in P(A-a)O2 and RI between group A and group B.Conclusion 4 mg/(kg· h)of propofol can significantly shorten the recovery time and awake time, improve the level of lipid metabolism and pulmonary function.

Development orientation and biocompatibility of dialysis-membrane materials / 中国组织工程研究

Hemodialysis is a major method to treat acute and chronic renal functional failure; while, dialysis-membrane is a major component of dialyzer, determining dialysis effect by the physical and chemical characteristics. In this study, we investigated the development orientation of hemodialysis, application of common membrane materials, and biocompatibility of dialysis-membrane materials; in addition, we also summarized the improvement of dialysis-membrane materials and the effect on hemodialysis during recent years.