Comparison of the effect of interlocking minimally invasive percutaneous plating and interlocking intramedul-lary nailing in the treatment of tibial fractures of the lower tibia / 中国基层医药

Objective To observe the effect of interlocking minimally invasive percutaneous plating and interlocking intramedullary nailing in treating fractures of the lower tibia,thus to guide clinical treatment.Methods A retrospective analysis of 70 patients with tibial fractures of the lower tibia were made.All the patients were divided into interlocking minimally invasive percutaneous plating group (A group)and interlocking intramedullary nailing group (B group),35 cases in each group.The operative time,blood loss,fracture healing time,hospitalization time were compared,the ankle score after 1 month,3 months,6 months,9 months,12 months were recorded,the complica-tions(loose,broken nails,deep infection,superficial infection,delayed union,malunion,joint pain)were observed,the Johner Wruh method was used to evaluate the clinical efficacy of postoperative tibial functional recovery.Results The operative time of A group and B group were (130.2 ±40.1)min and (96.4 ±30.5)min,A group was signifi-cantly longer than B group (t =3.853,P 0.05).There were no differ-ences between A group and B group in the ankle score after 1 month,3 months,6 months,9 months,12 months(t =0.855,1.315,1.527,0.787,0.885,both P >0.05).The clinical efficacy of tibia functional recovery in A group and B group were 97.14% and 94.29%,the difference was not statistically significant (χ2 =0.354,P >0.05).The inci-dence rates of loose,broken nails,deep infection,superficial infection,delayed healing in A group and B group had no difference (χ2 =0,0.348,1.014,3.134,0.348,all P >0.05),the incidence rates of malunion and joint pain in A group and B group were 2.86% and 20%,25.71% and 48.57%,which of A group were significantly lower than B group,the differences were statistically significant (χ2 =5.081,3.916,all P <0.05).Conclusion Interlocking min-imally invasive percutaneous plating and interlocking intramedullary nailing have exact effect in treatment of tibial fractures of the lower tibia,the effect of both methods has no difference,but interlocking minimally invasive percutane-ous plating has lower rate of malunion and knee pain,and suits for fracture patients with good soft tissue condition,but interlocking intramedullary nailing suits for fracture patients with poor soft tissue condition,fracture patients with good soft tissue condition and simple fracture,should master surgical indications of both surgery.

Construction of Hi FGF2 eukaryotic expression plasmids and its over-expression induced cell apoptosis / 中国药理学通报

Aim To construct eukaryotic expressing plasmid of hi FGF2 ( high molecular weight isoform fi-broblast growth factor-2,hi FGF2) gene and to investi-gate its effect on apoptosis after its overexpression in HEK293 cells. Methods The DNA template primer was designed and synthesized. The pDsRed1-N1 plas-mids were digested by the restriction enzymes of Nhel and Hind III. The hi FGF2 was ligated with linearized pDsRed1-N1 by T4 DNA Ligase. The recombinant plasmid was identified by endonuclease digestion and sequenced. The recombinant hi FGF2 plasmid was transient transfected into HEK293 cells by Lipofectami-neTM 2000 Reagent. The transfection efficiency was de-tected by fluorescence inversion microscope. The cell apoptosis was detected by Annexin V-FITC/PI apopto-sis detection kit with flow cytometry analysis. Results The pDsRed1-N1 eukaryotic expression vector was consistent with the design. The recombinant hi FGF2 plasmid was transfected in HEK293 cells. The trans-fection rate was more than 70%. The FITC/PI dyeing rate in hi-FGF2 over-expression HEK297 cells was a-bout ( 29. 12 ± 2. 81 )%. Conclusions pDsRed1-N1 eukaryotic expression vector is successfully constructed and transfected into HEK293 cells. Over-expression of hi FGF2 induces cell apoptosis.

Protease-activated receptor 1 challenges human lung epithelial cells to produce MCP-1 / 第三军医大学学报

Objective To investigate the actions of PAR1 agonists and thrombin on the secretion of monocyte chemoattractant protein (MCP)-1 from human lung epithelial cells. Methods A549 cells were cultured in a 12-well culture plate. The challenge was performed by addition of various concentrations of PAR1 agonist peptides SFLLR and its reverse peptides RLLFS, thrombin or thrombin inhibitor named hirudin into each well, respectively. After 2 h or 16 h, the reactions were terminated by removal of the supernatant from each well. A sandwich ELISA was used to determine the levels of MCP-1 in supernatants. Results Following 16 h incubation, SFLLR could induce concentration-dependent secretion of MCP-1. The maximum release of MCP-1 was nearly 12-fold more than baseline release. The reverse PAR1 agonists had little effects on MCP-1 release. Thrombin could induce concentration-dependent secretion of MCP-1. As low as 3 000 U/L thrombin could induce MCP-1 release from epithelial cells, and the maximum of accumulated release of MCP-1 was observed with 10 000 U/L thrombin, which was 5-fold more than baseline release. Thrombin inhibitor hirudin could inhibit thrombin induced secretion of MCP-1. The time course showed that the actions of PAR1 agonist peptides SFLLR and thrombin initiated at 2 h and reached their peak at 16 h. Conclusion PAR1 agonist peptides and thrombin are potent secretogogue of MCP-1 release from cultured human lung epithelial cells, and PAR1 antagonists and thrombin inhibitor may possess the ability to inhibit airway inflammation.