RESUMO
Objective To study the correlation between serum cysteine aspartate proteinase 2(CASP2)level and neonatal behavioral neurological assessment(NBNA)score in children with hypoxic-ischemic en-cephalopathy(HIE),and the value of CASP2 in evaluating prognosis.Methods A total of 114 neonates with HIE in this hospital from January 2018 to April 2022 were selected as HIE group.According to the severity of the disease,the neonates were divided into mild group(40 cases),moderate group(52 cases)and severe group(22 cases).According to the prognosis after 6 months of treatment,114 neonates with HIE were divided into poor prognosis group(26 cases)and good prognosis group(88 cases).At the same time,106 healthy neonates born in the hospital were selected as the control group.The NBNA score was recorded,and the serum CASP2 level was detected by enzyme-linked immunosorbent assay.Pearson correlation was used to analyze the corre-lation between serum CASP2 level and NBNA score in HIE children.Receiver operating characteristic(ROC)curve was used to analyze the predictive value of NBNA score and serum CASP2 level for the prognosis of ne-onates with HIE.Results The level of serum CASP2 in HIE group was higher than that in control group,and the NBNA score was lower than that in control group(P<0.05).The level of serum CASP2 in the severe group was higher than that of the moderate group and mild group,and the NBNA score was lower than that of moderate group and mild group(P<0.05).The level of serum CASP2 in the moderate group was higher than that of the mild group,and the NBNA score was lower than that of the mild group(P<0.05).The NBNA score was negatively correlated with the serum level of CASP2 in the children with HIE(r=-0.608,P<0.05).The poor prognosis group had a significantly higher serum level of CASP2 and a significantly lower NBNA score than the good prognosis group(P<0.05).The serum level of CASP2 and NBNA score separate and joint area under the curve of the poor children with HIE prognosis were 0.875(95%CI 0.797-0.952),0.748(95%CI 0.639-0.857),0.927(95%CI 0.873-0.981).Conclusion The level of serum CASP2 increases with the severity of HIE,and is closely related to the NBNA score,which has an important prognostic value.
RESUMO
Objective To explore the role of compound nerve conduit, made of nerve growth factors (NGF) encapsuled by biodegradable core-shell nanofibers through coaxial electrospinning, in regeneration of injured sciatic nerves in rats. Methods The compound nerve conduits were developed from the core-shell structured biodegradable nanofibers with P(LLA-CL) as a shell and BSA/NGF or BSA as a core through coaxial electrospinning. Seventy-two Sprague-Dawley rats were randomly divided into 4 even groups. The middle segments (10 mm) of the sciatic nerve were excised and the defects were repaired with sciatic nerve autograft (group A), with P(LLA-CL) conduit (group B), with PLLA-CL conduit and one injection of NGF (group C), and with P(LLA-CL)/NGF controlled-release conduit (group D), respectively. Morphologic and functional evaluations of nerve regeneration were done by gross observation, sciatic function index, neural electrophysiological examination, resumption rates of triceps weight, histological and ultrastructural observa-tion respectively in 1, 2, 3 months after the operation. Results Three months after the operation, although partial biodegradation and small cracks could be observed, conduits remained intact in outline. Based on the functional and histological observations, nerve regeneration, nerve fibers arrangement, myelination and nerve function reconstruction in the P(LLA-CL)/NGF controlled-release conduit (group D) were similar to those in nerve autograft (group A) and significantly superior to those in groups B and C (P<0.05). Conclusion As the P(LLA-CL)/NGF-controlled release conduit has favorable mechanical properties and biocompatibility, it can effectively promote regeneration of the sciatic nerve in rats.