Study on Chemical Constituents of Dioscorea opposita Thunb / 世界科学技术-中医药现代化

This article was aimed to study the chemical constituents of Dioscorea opposita Thunb.The chemical constituents were isolated and purified by Diaion HP-20,Toyopearl HW-40,Sephadex LH-20,MCI Gel CHP-20,silica gel column chromatography and preparative HPLC,TLC,purification and isolation from Dioscorea opposita Thunb.The structures of isolated compounds were identified by thc physicochemical properties and spectral analysis.The result showed that 14 compounds were isolated from Dioscorea opposita Thunb.The chemical structures were elucidated as L-Tryptophane (1),Seguinosides F (2),1-methoxycarbonyl-β-carboline (3),Helichrysin A (4),Bungein A (5),Hydroquinone (6),Zarzissine (7),Cyclo-(Pro-Thr) (8),4-Hydroxy-3-methoxybenzyl alcohol (9),pyridine-3-carboxamide (10),Arbutin (11),Methyl syringate 4-O-β-D-glucopyranoside (12),L-Phenylalanine (13),1,2-benzenedicarboxylic acid,1,2-bis[2-(2-hydroxyethoxy) ethyl] ester (14).It was concluded that chemical compounds 1-14 were isolated for the first time from Dioscorea opposita Thunb.

Effect of WeChat on compliance of COPD patients after discharge / 现代临床护理

Objective To investigate the influence of WeChat on compliance of chronic obstructive drsease(COPD)patients after discharge.Methods Eighty COPD patients were divided into observation group and control group in equal number.The former received health education by WeChat, while the control group regular revisits.The two groups were compared in terms of the compliance in mediation and dieting as well as readmission.Result The rate of compliance in mediation and dieting in the observation group was significantly higher than that in the control and the rate of readmission significantly lower than that in the control group(P<0.05). Conclusion Health education by way of WeChat for COPD patients can enhance their compliance in medication and consolidate the curative effect.

Study on correlation between ITS sequence of Arctium lappa and quality of Fructus Arctii / 中国中药杂志

<p><b>OBJECTIVE</b>To study the correlation between ITS sequence of Arctium lappa and Fructus Arctii quality of different origin.</p><p><b>METHOD</b>The samples of Fructu arctii materials were collected from 26 different producing areas. Their ITS sequence were determined after polymerase chain reaction (PCR) and quality were evaluated through the determination of arctiin content by HPLC. Genetic diversity, genotype and correlation were analyzed by ClustalX (1.81), Mage 4.0, SPSS 13.0 statistical software.</p><p><b>RESULT</b>ITS sequence of A. was obtained from 26 samples, and was registered in the GenBank. Corresponding arctiin content of Fructus arctii and 1000-grain weight were determined. A. lappa genotype correlated with Fructus arctii quality by statistical analysis.</p><p><b>CONCLUSION</b>The research provided a foundation for revealing the molecular mechanism of Fructus arctii geoherbs.</p>

Identification of traditional medicine "Fructus Arctii" by nuclear ribosomal DNA ITS sequences / 中国中药杂志

<p><b>OBJECTIVE</b>To identify the traditional medicine Fructus Arctii from its adulterants by ITS.</p><p><b>METHOD</b>Twenty-six samples of the different Fructus Arctii materials and 10 samples of the adulterants of the fruits of A. tomentosum, Onopordum acantium, Silybum marianum, and Amorpha fruticosa were collected. The total DNA of the samples were extracted, amplified cloned and sequenced.</p><p><b>RESULT</b>ITS sequences were obtained from 26 samples respectively, there were Fructus Arctii 641 bp, A. tomentosum 641 bp, Onopordum acantium 639 bp, Silybum marianum 630-631 bp, Amorpha fruticosa 625-626 bp, which were registered in the GenBank. The similarity in ITS sequences between Fructus Arctii and the adulterants was less than 95%. In contrast, the similarity between any pair of Fructus Arctii was greater than 99%. The similarity was 98.29%-99.22% between Fructus Arctii and A. tomentosum. Phylogeny tree reconstruction using UPGMA analysis based on ITS nucleotide sequences can effectively distinguish Fructus Arctii from adulterants.</p><p><b>CONCLUSION</b>ITS sequences can be used as a reliable molecular marker for the identification of Fructus Arctii.</p>