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International Journal of Oral Science ; (4): 227-232, 2014.
Artigo em Inglês | WPRIM | ID: wpr-358133

RESUMO

The time domain entombment of bacteria by intratubular mineralization following orthograde canal obturation with mineral trioxide aggregate (MTA) was studied by scanning electron microscopy (SEM). Single-rooted human premolars (n=60) were instrumented to an apical size #50/0.06 using ProFile and treated as follows: Group 1 (n=10) was filled with phosphate buffered saline (PBS); Group 2 (n=10) was incubated with Enterococcus faecalis for 3 weeks, and then filled with PBS; Group 3 (n=20) was obturated orthograde with a paste of OrthoMTA (BioMTA, Seoul, Korea) and PBS; and Group 4 (n=20) was incubated with E. faecalis for 3 weeks and then obturated with OrthoMTA-PBS paste. Following their treatments, the coronal openings were sealed with PBS-soaked cotton and intermediate restorative material (IRM), and the roots were then stored in PBS for 1, 2, 4, 8 or 16 weeks. After each incubation period, the roots were split and their dentin/MTA interfaces examined in both longitudinal and horizontal directions by SEM. There appeared to be an increase in intratubular mineralization over time in the OrthoMTA-filled roots (Groups 3 and 4). Furthermore, there was a gradual entombment of bacteria within the dentinal tubules in the E. faecalis inoculated MTA-filled roots (Group 4). Therefore, the orthograde obturation of root canals with OrthoMTA mixed with PBS may create a favorable environment for bacterial entombment by intratubular mineralization.


Assuntos
Humanos , Compostos de Alumínio , Usos Terapêuticos , Calcificação Fisiológica , Fisiologia , Compostos de Cálcio , Usos Terapêuticos , Cristalização , Cavidade Pulpar , Microbiologia , Dentina , Microbiologia , Combinação de Medicamentos , Enterococcus faecalis , Metilmetacrilatos , Usos Terapêuticos , Microscopia Eletrônica de Varredura , Óxidos , Usos Terapêuticos , Materiais Restauradores do Canal Radicular , Usos Terapêuticos , Obturação do Canal Radicular , Métodos , Preparo de Canal Radicular , Silicatos , Usos Terapêuticos , Fatores de Tempo , Cimento de Óxido de Zinco e Eugenol , Usos Terapêuticos
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