MiR-135b promotes proliferation of endometrial carcinoma cells by targeting FOXO1 / 南方医科大学学报

<p><b>OBJECTIVE</b>To explore the expression of miR-135b in endometrial carcinoma and the mechanism by which miR-135b promotes the proliferation of endometrial cancer cells.</p><p><b>METHODS</b>The expressions of miR-135b and FOXO1 were using RT-PCR detected in 22 fresh endometrial cancer tissues and paired adjacent tissues and also in endometrial cancer cell lines JEC, Ishikawa, HEC-1-B, and RL-952. The RL-952 and Ishikawa cell lines were transfected with miR-135b mimics or inhibitors, and the changes in their proliferative activity were detected with MTT assay; the expressions of FOXO1 mRNA and protein were detected by RT-PCR and Western blotting, respectively.</p><p><b>RESULTS</b>The expression of miRNA135b was significantly up-regulated and FOXO1 expression was down-regulated in endometrial carcinoma tissues as compared with the adjacent tissues (P<0.05). The mRNA expression of miR-135b was negatively correlated with the expression of FOXO1 in endometrial carcinoma. In RL-952 and Ishikawa cell lines, transfection with miR-135b mimics obviously promoted the cell proliferation (P<0.05). Up-regulation of miR-135b significantly decreased the expressions of FOXO1 protein and mRNA (P<0.05), and down- regulation of miR-135b increased FOXO1 expressions (P<0.05).</p><p><b>CONCLUSIONS</b>MiR-135b plays an important role in the occurrence and development of endometrial carcinoma partially by regulating its target gene FOXO1.</p>

Tip and torque changes in maxillary buccal segment after orthodontic treatment: a three-dimensional study / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To investigate three-dimensional imaging registration and superimposition techniques in measuring the tip and torque change of upper canine, premolar and first molar after orthodontic treatment.</p><p><b>METHODS</b>Twenty-eight subjects (14 extraction cases and 14 non-extraction cases) with full records were randomly selected from the Department of Orthodontics, Peking University School and Hospital of Stomatology. The pre-and post-treatment upper dental casts were digitized with three-dimensional spot laser scanner and superimposed with reverse engineering software. The facial axis of the clinical crown (FACC) was transferred from post-treatment teeth to the pre-treatment teeth using three-dimensional imaging registration. The occlusal plane was constructed on the post-treatment upper digital cast and the tip and torque values were measured.</p><p><b>RESULTS</b>In the non-extraction group, the tip of the second premolar decreased by 1.5° (P < 0.05). The buccal crown torque of the first and second premolars increased by 5.1° and 4.2° (P < 0.05), respectively. In the extraction group, the lingual crown torque of upper canine increased by 3.8° (P < 0.05).</p><p><b>CONCLUSIONS</b>Non-extraction orthodontic treatment tended to tip the upper second premolar distally and increased the buccal crown torque of the upper premolars while extraction treatment increased the lingual crown torque of the upper canine.</p>

Association of transforming growth factor-alpha gene polymorphism and environment factors with nonsyndromic cleft lip with or without cleft palate in Han nationality / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To study the association of transforming growth factor-alpha (TGF-alpha gene polymorphism and environment factors with nonsyndromic cleft lip with or without cleft palate (NSCLP) in Han nationality.</p><p><b>METHODS</b>Data related to infection, drug intake and folic acid supplement during pregnancy were gained through investigation of mothers. Polymerase chain reaction combined with restrict enzyme digestion was used to detect the target gene variation in 199 patients with NSCL/P and 203 healthy controls. Analysis was carried on the genotype and infection,drug intake and folic acid supplement.</p><p><b>RESULTS</b>The C2 allele frequency in patients with NSCL/P was significantly higher than that in healthy controls. There was a significant increase of patients with NSCL/P in pregnant women exposed to infection, drug intake and folic acid deficiency. There was an interaction between C1C2 genetype and infection, drug intake and folic acid supplement.</p><p><b>CONCLUSION</b>TGF-alpha gene polymorphism is associated with NSCL/P. Infection, drug intake and folic acid supplement during pregnancy were associated with the occurrence of NSCL/P. Individuals containing C2 allele were more sensitive to infection, drug intake and folic acid deficiency.</p>