Genetic Structure Analysis of Y Chromosome STR and SNP in Population of Changshu / 法医学杂志

Objective To provide a theoretical basis for building a Y chromosome database in specific regions by analyzing the pedigree specific core haplogroup and region specific genetic structure in Changshu. Methods One thousand seven hundred and two samples from unrelated Han male individuals in Changshu were collected. Then 27 Y-STR were genotyped through YfilerTM Plus PCR Amplification Kit, Y-SNP haplogroup of each sample was speculated using Y-Predictor software and some samples were verified by amplification refractory mutation system-polymerase chain reaction （ARMS-PCR）. Results A total of 1 556 haplotypes were found on the 27 Y-STR genetic markers of the 1 702 samples. The haplotype diversity （HD） value was 0.999 827. DYS385 （0.933） had the highest gene diversity （GD） value while DYS438 （0.409） had the lowest. By the Y-Predictor software, all samples were confirmed to be from 162 sub-haplogroups of C, D, N, O, Q and R. Samples were randomly selected to verify the prediction results by the software and the prediction accuracy of Y-Predictor software was as high as 95.74%. Conclusion This study found that 27 Y-STR genetic markers have relatively high polymorphisms in the Changshu population, and have good forensic individual identification and paternity testing ability.

Genetic Structure Analysis of Y-Chromosome STR and SNP in Population of Wujiang Area, Suzhou City / 法医学杂志

Objective To analyze the genetic phenotypes of Y-chromosome STR and SNP in Han male population of Wujiang area, Suzhou City and explore the genetic structure of population of Wujiang area for further examination of regional-specific Y-SNP genetic markers ancestor haplogroups. Methods Blood samples of 472 Wujiang area Han males were randomly collected and genotyped by YfilerTM Plus PCR Amplification Kit. The allele frequencies and haplotype frequencies of each locus were obtained using the direct calculation method. Y-SNP haplogroups of each sample were estimated using Y-Predictor software and verified through experiments by amplification refractory mutation system-polymerase chain reaction （ARMS-PCR）. Results A total of 453 haplotypes were found in the 27 Y-STR genetic markers in 472 Han males of Wujiang area. The haplotype diversity （HD） was 0.997 696 93, among which, the highest gene diversity （GD） value was DYF387S1a/b （GD=0.953 1） and the lowest was DYS438 （GD=0.321 8）. Based on genotyping data of 27 Y-STRs and 472 samples, 132 haplogroups from C, D, N, O and Q, etc downstream Y-SNP haplogroups were estimated and then verified through experiments. Conclusion This study is based on Y-chromosome STR haplotypes, and predicts Y-SNP haplogroups by Y-Predictor software, then uses ARMS-PCR to verify. Y-SNP genetic markers were introduced to achieve precise analysis of the genetic structure of male families in population of three towns in Wujiang area.

Clinical effect of Saccharomyces boulardii powder combined with azithromycin sequential therapy in treatment of children with diarrhea secondary to Mycoplasma pneumoniae pneumonia / 中国当代儿科杂志

<p><b>OBJECTIVE</b>To investigate the clinical effect of Saccharomyces boulardii powder combined with azithromycin sequential therapy in the treatment of children with diarrhea secondary to Mycoplasma pneumoniae pneumonia.</p><p><b>METHODS</b>A total of 88 children with diarrhea secondary to Mycoplasma pneumoniae pneumonia between June 2015 and March 2017 were divided into control group and study group using a random number table, with 44 children in each group. The children in the control group were given routine treatment combined with azithromycin sequential therapy, and those in the study group were given oral Saccharomyces boulardii powder in addition to the treatment in the control group until the end of azithromycin sequential therapy. After the treatment ended, the two groups were compared in terms of time to improvement of clinical symptoms, length of hospital stay, clinical outcome, defecation frequency before and after treatment, condition of intestinal dysbacteriosis, and incidence of adverse events.</p><p><b>RESULTS</b>Compared with the control group, the study group had significantly shorter time to improvement of clinical symptoms and length of hospital stay (P<0.05). The study group had a significantly higher response rate than the control group (P<0.05). On days 3 and 5 of treatment, the study group had a significant reduction in defecation frequency compared with the control group (P<0.05). The study group had a significantly lower rate of intestinal dysbacteriosis than the control group (P<0.05). There was no significant difference in the incidence of adverse events between the two groups (P>0.05).</p><p><b>CONCLUSIONS</b>In the treatment of children with diarrhea secondary to Mycoplasma pneumoniae pneumonia, Saccharomyces boulardii powder combined with azithromycin sequential therapy can improve clinical symptoms, shorten the length of hospital stay, reduce defecation frequency and the incidence of intestinal dysbacteriosis, and improve clinical outcomes, and does not increase the risk of adverse events.</p>

A comparative study on the mechanical properties of flexible denture materials containing different fluoride concentrations / 中国组织工程研究

BACKGROUND: Long-term use of flexible denture prosthesis will increase the incidence of caries and periodontal disease, and partial denture stomatitis. The addition of fluoride in the prosthesis material can prevent and reduce the incidence of these diseases. Currently, increasing research addresses the addition of fluoride into dental materials at home and abroad, but little is reported on the addition of sodium monofluorophosphate (Na2(PO3)F into flexible denture materials and on the relevant mechanical performance.OBJECTIVE: To evaluate the mechanical properties of flexible denture materials containing different fluoride concentrations.METHODS: Totally 180 elastic denture samples were made at the three different sizes of 80 mm×51 mm×1 mm (n=60), 20 mm×5 mm×5 mm (n=60), 25 mm×2 mm×2 mm (n=60). Then, elastic denture samples containing Na2(PO3)F 0 (control), 3%, 5%, 10%, 15%, 20% were prepared in each size groups. The tensile strength, flexural strength and Shore A hardness of the samples were tested.RESULTS AND CONCLUSION: When the mass ratio of fluoride was up to 10%, the tensile strength and flexural strength reduced a little (P > 0.05). When the mass ratio of fluoride was up to 15%, the tensile strength and flexural strength were significantly lower than those of non-fluoride samples (P < 0.05). When the mass ratio of fluoride was up to 20%, the tensile strength, flexural strength and Shore A hardness were significantly lower than those of non-fluoride samples (P < 0.05). To conclude, Na2(PO3)F content has a significant effect on the tensile strength, flexural strength and Shore A hardness, and 10% is the best proportion of Na2(PO3)F. The mechanical properties of fluoride-containing flexible denture materials will be changed if the mass ratio of fluoride is higher than 10%.

A comparative study on the mechanical properties of flexible denture materials containing different fluoride concentrations / 中国组织工程研究

BACKGROUND: Long-term use of flexible denture prosthesis will increase the incidence of caries and periodontal disease, and partial denture stomatitis. The addition of fluoride in the prosthesis material can prevent and reduce the incidence of these diseases. Currently, increasing research addresses the addition of fluoride into dental materials at home and abroad, but little is reported on the addition of sodium monofluorophosphate (Na2(PO3)F into flexible denture materials and on the relevant mechanical performance.OBJECTIVE: To evaluate the mechanical properties of flexible denture materials containing different fluoride concentrations.METHODS: Totally 180 elastic denture samples were made at the three different sizes of 80 mm×51 mm×1 mm (n=60), 20 mm×5 mm×5 mm (n=60), 25 mm×2 mm×2 mm (n=60). Then, elastic denture samples containing Na2(PO3)F 0 (control), 3%, 5%, 10%, 15%, 20% were prepared in each size groups. The tensile strength, flexural strength and Shore A hardness of the samples were tested.RESULTS AND CONCLUSION: When the mass ratio of fluoride was up to 10%, the tensile strength and flexural strength reduced a little (P > 0.05). When the mass ratio of fluoride was up to 15%, the tensile strength and flexural strength were significantly lower than those of non-fluoride samples (P < 0.05). When the mass ratio of fluoride was up to 20%, the tensile strength, flexural strength and Shore A hardness were significantly lower than those of non-fluoride samples (P < 0.05). To conclude, Na2(PO3)F content has a significant effect on the tensile strength, flexural strength and Shore A hardness, and 10% is the best proportion of Na2(PO3)F. The mechanical properties of fluoride-containing flexible denture materials will be changed if the mass ratio of fluoride is higher than 10%.